Predicting oil accumulation by fruit image processing and linear models in traditional and super high-density olive cultivars.

The paper focuses on the seasonal oil accumulation in traditional and super-high density (SHD) olive plantations and its modelling employing image-based linear models. For these purposes, at 7-10-day intervals, fruit samples (cultivar Arbequina, Fasola, Frantoio, Koroneiki, Leccino, Maiatica) were pictured and images segmented to extract the Red (R), Green (G), and Blue (B) mean pixel values which were re-arranged in 35 RGB-derived colorimetric indexes (). After imaging, the samples were crushed and oil concentration was determined (NIR).

High-resolution gene expression atlases of two contrasting major Greek olive (Olea europaea L.) tree cultivars for oil and table olive production.

Description Of Aims And Systems Used: Olive (Olea europea L.) is one of the most economically important tree crops worldwide, especially for the countries in the Mediterranean basin. Given the economic and nutritional importance of the crop for olive oil and drupe production, we generated transcriptional atlases for the Greek olive cultivars "Chondrolia Chalkidikis" and "Koroneiki" which have contrasting characteristics in terms of fruit development, oil production properties, and use.

Response of L. (Cretan dittany) to Five Species of Root-Knot Nematodes ( spp.).

Cretan dittany ( L.) is an aromatic and medicinal plant, local endemic of the island of Crete, Greece, occurring naturally to high rocky mountain habitats. Due to its commercial interest, cultivation of this plant has been recently expanded.

Evaluation of phenolics in the analysis of virgin olive oil using near infrared spectroscopy.

Olive oil is an indispensable part of the diet in Mediterranean regions, and is appreciated worldwide for its sensory characteristics, combining a fine aroma and pleasant flavor with the high nutritional value of specific chemical compounds. However, making rapid measurements of phenolic compounds is a major challenge for the olive oil sector. The development of a new method based on near infrared (NIR) spectroscopy may be considered an important advance for the sector, as it is rapid, low-cost, non-contaminant and non-destructive.

The Elimination of Viroids through In Vitro Thermotherapy and a Meristem Tip Culture from a New Limonime Hybrid ( x var. (L.) Burm. f. x var. ).

Viruses and viroids pose a significant challenge in citriculture, and their control is crucial for plant health. This study evaluated the effectiveness of in vitro thermotherapy combined with a meristem tip culture for eliminating citrus exocortis viroid (CEVd) and hop stunt viroid (HSVd) from a new limonime hybrid ( x var. x var.

Rapid real-time quantitative colorimetric LAMP methodology for field detection of Verticillium dahliae in crude olive-plant samples.

Background: Verticilium dahliae is the most important wilt pathogen of olive trees with a broad host range causing devastating diseases currently without any effective chemical control. Traditional detection methodologies are based on symptoms-observation or lab-detection using time consuming culturing or molecular techniques. Therefore, there is an increasing need for portable tools that can detect rapidly V.

Transcriptomic Approach for Investigation of spp. Resistance upon Early-Stage Broomrape Parasitism.

Tomato () is a major horticultural crop of high economic importance. and genera (broomrapes) are parasitic weeds, constituting biotic stressors that impact tomato production. Developing varieties with tolerance to broomrapes has become imperative for sustainable agriculture.

First Report of causing root rot of avocado trees in Greece.

In September 2023, thirty declining 30-year-old avocado (Persea americana) trees ('Hass' grafted on 'Zutano' seedlings) were detected in a 1.5-ha orchard in the island of Crete (Chania region). Crown symptoms encompassed wilting and leaf chlorosis, advancing to defoliation and extensive dieback.

Gene Expression Profiling and Qualitative Characteristics in Delaying Flesh Softening of Avocado Fruits.

In this research, qualitative characteristics were studied under different post-harvest treatments in Hass and Fuerte cultivars of avocado () fruits. The post-harvest treatments performed in fruits of these cultivars comprised Ethrel application and plastic film (membrane) covering. The measurements of qualitative characteristics were related to color; flesh consistency; measurements of titratable acidity, total soluble solids, percentage of total phenolic contents, and ascorbic peroxidase activity; and the real-time (quantitative) polymerase chain reaction (qPCR) of gene expression and enzyme activities of phenylalanine ammonia-lyase (PAL) and beta-galactosidase (β-gal).

Optimizing Agroecological Measures for Climate-Resilient Olive Farming in the Mediterranean.

In order to evaluate the potential of climate change mitigation measures on soil physiochemical properties, an experiment based on the application of five agroecological practices such as the addition of composted olive-mill wastes, recycling pruning residue, cover crops, organic insect manure, and reduced soil tillage, solely or combined, was conducted over two years (2020 to 2022) in a 48-year-old olive plantation. The results showed significant increases in soil water content during the spring and summer periods for the combined treatment (compost + pruning residue + cover crops) (ALL) compared to the control (CONT) by 41.6% and 51.

Crop Landraces and Indigenous Varieties: A Valuable Source of Genes for Plant Breeding.

Landraces and indigenous varieties comprise valuable sources of crop species diversity. Their utilization in plant breeding may lead to increased yield and enhanced quality traits, as well as resilience to various abiotic and biotic stresses. Recently, new approaches based on the rapid advancement of genomic technologies such as deciphering of pangenomes, multi-omics tools, marker-assisted selection (MAS), genome-wide association studies (GWAS), and CRISPR/Cas9 gene editing greatly facilitated the exploitation of landraces in modern plant breeding.

Comparative transcriptome profiling and co-expression network analysis uncover the key genes associated with pear petal defense responses against infection.

Pear brown rot and blossom blight caused by seriously affect pear production worldwide. Here, we compared the transcriptomic profiles of petals after inoculation with using two pear cultivars with different levels of sensitivity to disease (Sissy, a relatively tolerant cultivar, and Kristalli, a highly susceptible cultivar). Physiological indexes were also monitored in the petals of both cultivars at 2 h and 48 h after infection (2 HAI and 48 HAI).

Global Transcriptome Analysis of the Peach () in the Interaction System of Fruit-Chitosan-.

The peach ( L.) is one of the most important stone-fruit crops worldwide. Nevertheless, successful peach fruit production is seriously reduced by losses due to the causal agent of brown rot.

DNA Barcoding and Fertilization Strategies in subsp. , a Local Endemic Plant of Crete with High Medicinal Value.

Herein, we applied DNA barcoding for the genetic characterization of subsp. (Lamiaceae; threatened local Cretan endemic plant) using seven molecular markers of cpDNA. Five fertilization schemes were evaluated comparatively in a pilot cultivation in Crete.

Grapevine Trunk Diseases in Greece: Disease Incidence and Fungi Involved in Discrete Geographical Zones and Varieties.

A three-year survey was conducted to estimate the incidence of grapevine trunk diseases (GTDs) in Greece and identify fungi associated with the disease complex. In total, 310 vineyards in different geographical regions in northern, central, and southern Greece were surveyed, and 533 fungal strains were isolated from diseased vines. Morphological, physiological and molecular (5.

Genetic and Epigenetic Responses of Autochthonous Grapevine Cultivars from the 'Epirus' Region of Greece upon Consecutive Drought Stress.

Within the framework of preserving and valorizing the rich grapevine germplasm of the Epirus region of Greece, indigenous grapevine ( L.) cultivars were characterized and assessed for their resilience to abiotic stresses in the context of climate change. The cultivars 'Debina' and 'Dichali' displayed significant differences in their response to drought stress as judged by morpho-physiological analysis, indicating higher drought tolerance for Dichali.

Wild and cultivated olive tree genetic diversity in Greece: a diverse resource in danger of erosion.

The genetic relationships between Greek wild olive tree populations and cultivars were investigated. A total of 219 wild genotypes and 67 cultivar genotypes were analyzed by employing 10 SSR markers. Data evidenced that the wild populations exhibited high levels of genetic diversity and exclusively host 40% of the total number of alleles detected.

One hundred single-copy nuclear sequence markers for olive variety identification: a case of fingerprinting database construction in China.

Unlabelled: Olive is an ancient oil-producing tree, widely cultivated in Mediterranean countries, and now spread to other areas of the world, including China. Recently, several molecular databases were constructed in different countries and platforms for olive identification using simple sequence repeats (SSRs) or single-nucleotide polymorphisms (SNPs). However, comparing their results across laboratories was difficult.

First reports of , and (Tylenchomorpha) from Greece and further records of four other nematode species.

Nematode samplings in various areas and crops of Greece were carried out and the recovered nematode species were characterized using morphological and molecular data. Seven species of plant-parasitic nematodes were recovered, three of which are reported for the first time in Greece, including , and . Four other recovered species had already been reported in Greece, including , , and .

Physiological Responses of Two Olive Cultivars to Salt Stress.

The olive tree ( L.) is the main fruit tree in most of the arid and semi-arid regions of Tunisia, which is where the problem of salinity is more pronounced. Salinity is one of the main factors that affects the productivity of olive trees, so the objective of this experiment was to study the effects of salinity on the photosynthesis, water relations, mineral status, and enzymatic activity of two cultivars of L.

Integrated transcriptome and proteome analysis the molecular mechanisms of nutritional quality in 'Chenggu-32' and 'Koroneiki' olives fruits (Olea europaea L.).

In this work, integrated transcriptome and proteome to offer a new insight of the molecular mechanisms linked to the nutritional quality of Koroneiki and Chenggu-32 by RNA sequencing and 4D Label-free quantitative proteomics technology. Physical and chemical properties studies showed that the main nutrient content of Koroneiki was significantly higher than Chenggu-32, proved the quality of Koroneiki was better. Compared to Koroneiki, there were differences in expression levels of 10,115 genes and 723 proteins in Chenggu-32, mainly related to enzymes in lipid metabolism and lipid biosynthesis.