Acute and subacute demyelination induced by mouse hepatitis virus strain A59 in C3H mice.

Mouse hepatitis virus (MHV, strain A-59), a coronavirus, induced acute and subacute demyelinating disease in the central nervous system of weanling C3H mice that were reported to be genetically resistant to MHV. Subtle clinical signs developed in greater than 90% of animals 5-7 days after intracerebral inoculation of 25 PFU, and foci of demyelination were detected from 1 to 4 weeks postinoculation (p.i.

Reproductive performance of a laboratory breeding colony of patas monkeys (Erythrocebus patas).

Reproductive statistics were gathered over a 5½-year period on a colony of Erythrocebus patas. Pregnancies occurred throughout the year under laboratory conditions with a suggestion of a mating peak in the late fall and early winter. Menstrual cycles were monitored and found to average 30.

Altered immune function in demyelinative disease.

Experimentally induced demyelination has an autoimmune origin in some mouse models and is somehow virally induced in others. In June, invited participants in a Kroc Foundation workshop discussed the damaging mechanisms involved and their relevance to an understanding of multiple sclerosis and human encephalomyelitis.

Larval rearing, metamorphosis, growth and reproduction of the eolid nudibranch hermissenda crassicornis (eschscholtz, 1831) (gastropoda: opisthobranchia).

1. Hermissenda crassicornis is a subannual nudibranch species that reproduces year-round. 2.

Cell binding and growth inhibition by hexachlorophene of decanoate and their reversibility.

More than 80% of the hexachlorophene added to a Bacillus subtilis culture binds to the cells. Complete growth inhibition requires 6 x 10(5) molecules bound per cell. In contrast, more than 99% decanoate remains in solution and 3.

Studies on the GABAergic system in astrocytoma and neuroblastoma cells in culture.

The GABAergic system was investigated in C-6 astrocytoma cells and C-1300 neuroblastoma cells in culture and compared to that in mouse brain. The activities of glutamate decarboxylase, GABA-transaminase, succinic semialdehyde dehydrogenase and glutamate dehydrogenase were measured. In the cultured cells, only glutamate dehydrogenase activity was equal or greater than that of mouse cerebral cortex.

The sarcolemma of Aplysia smooth muscle in freeze-fracture preparations.

Smooth muscle cells in the sheath covering the visceral ganglion of Aplysia californica were examined with the techniques of freeze-fracture and conventional electron microscopy. The sarcolemma of these muscle cell invaginates to form myriad caveolae that have an intrinsic marker within their membrane. This intrinsic structure of the caveolar membrane is revealed by freeze-fracture and consists of rows of large particles in the outer half and matching grooves on the complementary inner half of the membrane.

Adenosine deaminase of cultured brain cells.

Two types of adenosine deaminase (EC 3.5.4.

Seroepidemiology of human papovaviruses. Discovery of virgin populations and some unusual patterns of antibody prevalence among remote peoples of the world.

A total of 1544 sera from 28 diverse and mainly isolated populations were examined for HI antibody to BK virus. A few extremely isolated populations were found with negligible or absent exposure to the virus, but in most populations, remote or cosmopolitan, antibody appeared in increasing prevalence during early childhood and remained stable throughout adult life. Antibody acquisition and prevalence rates in individual families reflected that of the general population.