Structure in lissencephaly determined by immunohistochemical staining.

The brains of patients with lissencephaly were examined by peroxidase-antiperoxidase immunohistochemical staining of synaptophysin, myelin basic protein, and glial fibrillary acidic protein. In contrast to the normal cortical pattern, the cortex, with a smooth surface, demonstrated quite different staining patterns in the molecular, superficial cellular, sparsely cellular, and deep cellular layers. The molecular layer was abnormally positive with synaptophysin staining.

Neonatal cocaine-related seizures.

Cocaine abuse is associated with a variety of severe acute neurologic complications typically occurring in the abusers themselves. These include ischemic stroke, subarachnoid and intraparenchymal hemorrhage, headaches, syncope, seizures, and death. Sixteen pediatric patients with presumed cocaine-related seizures secondary to maternal consumption are reported.

Effects of barium and bicarbonate on glial cells of Necturus optic nerve. Studies with microelectrodes and voltage-sensitive dyes.

We have studied the effects of Ba++, a known K+ channel blocker, on the electrophysiological properties of the glial cells of Necturus optic nerve. The addition of Ba++ reversibly depolarized glial cells by 25-50 mV; the half maximal deplorization was obtained with a Ba++ concentration of approximately 0.3 mM.

Calcium channels that are required for secretion from intact nerve terminals of vertebrates are sensitive to omega-conotoxin and relatively insensitive to dihydropyridines. Optical studies with and without voltage-sensitive dyes.

Extrinsic absorption changes exhibited by potentiometric dyes have established the ionic basis of the action potential in synchronously activated populations of nerve terminals in the intact neurohypophyses of amphibia and mammals (Salzberg et al., 1983; Obaid et al., 1983, 1985b).