2 results match your criteria: "Institute of Microbiology of Chinese Academy of Sciences[Affiliation]"
[Effect of over-expression of sterol C-22 desaturase on ergosterol production in yeast strains].
Wei Sheng Wu Xue Bao
April 2007
Institute of Microbiology of Chinese Academy of Sciences, Beijing, 100080, China.
Article Synopsis
- Ergosterol is a key component of yeast membranes, affecting fluidity and permeability, and is also important for vitamin D2 production.
- The ERG5 gene encodes an enzyme essential for the formation of a double bond in the ergosterol biosynthesis pathway and was cloned and over-expressed in yeast for study.
- The study involved amplifying the ERG5 gene, creating a recombinant plasmid for expression, and comparing sterol profiles of yeast strains to see the effects of ERG5 over-expression on ergosterol production.
[Genetically modified industrial brewing yeast with high-glutathione and low-diacetyl production].
Sheng Wu Gong Cheng Xue Bao
November 2005
The Laboratory of Molecular Genetics and Breeding of Yeast, Institute of Microbiology of Chinese Academy of Sciences, Beijing, 100080, China.
Recombinant plasmid pICG was constructed by replacing the internal fragment of a-acetohydroxyacid synthase (AHAS) gene (ILV2) with a copy of gamma-glutamylcysteine synthetase gene (GSH1) and copper chelatin gene (CUP1) from the industrial brewing yeast strain YSF31. YSF31 was transformed with plasmid pICG linearized by Kpn I and Pst I. A recombinant strain with high-glutathione and low-diacetyl production was selected.
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