1 results match your criteria: "Institute of Medicine Henan University of Science and Tochnology[Affiliation]"
[Construction of lentiviral mediated CyPA siRNA and its functions in non-small cell lung cancer].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
February 2010
Institute of Medicine Henan University of Science and Tochnology, Luoyang 471003, China.
Objective: To construct a lentiviral-vector-mediated CyPA small interference RNA (siRNA) and study its function in non-small cell lung cancer.
Methods: First, four target sequences were selected according to CyPA mRNA sequence, the complementary DNA contained both sense and antisense oligonucleotides were designed, synthesized and cloned into the pGCL-GFP vector, which contained U6 promoter and green fluorescent protein (GFP). The resulting lentiviral vector containing CyPA shRNA was named Lv-shCyPA, and it was confirmed by PCR and sequencing.