Soluble receptor for advanced glycation end products protects from ischemia- and reperfusion-induced acute kidney injury.

The full-length receptor for advanced glycation end products (RAGE) is a multiligand pattern recognition receptor. High-mobility group box 1 (HMGB1) is a RAGE ligand of damage-associated molecular patterns that elicits inflammatory reactions. The shedded isoform of RAGE and endogenous secretory RAGE (esRAGE), a splice variant, are soluble isoforms (sRAGE) that act as organ-protective decoys.

Diagnostic value of RNA for hepatocellular carcinoma: a network meta-analysis.

The aim of this study was to evaluate the capacity of RNA in the diagnosis of hepatocellular carcinoma (HCC). A systematic review was conducted from PubMed, Cochrane Library, EMBASE and Web of Science databases via well-designed retrieval strategy. Subsequently, the network meta-analysis was performed by the STATA software.

A Nanofiber Mat With Dual Bioactive Components and a Biomimetic Matrix Structure for Improving Osteogenesis Effect.

The challenge of effectively regenerating bone tissue through tissue engineering technology is that most tissue engineering scaffolds cannot imitate the three-dimensional structure and function of the natural extracellular matrix. Herein, we have prepared the poly(L-lactic acid)-based dual bioactive component reinforced nanofiber mats which were named as poly(L-lactic acid)/bovine serum albumin/nanohydroxyapatite (PLLA/BSA/nHAp) with dual bioactive components by combining homogeneous blending and electrospinning technology. The results showed that these nanofiber mats had sufficient mechanical properties and a porous structure suitable for cell growth and migration.

The GPI-anchored protein CD109 protects hematopoietic progenitor cells from undergoing erythroid differentiation induced by TGF-β.

Although a glycosylphosphatidylinositol-anchored protein (GPI-AP) CD109 serves as a TGF-β co-receptor and inhibits TGF-β signaling in keratinocytes, the role of CD109 on hematopoietic stem progenitor cells (HSPCs) remains unknown. We studied the effect of CD109 knockout (KO) or knockdown (KD) on TF-1, a myeloid leukemia cell line that expresses CD109, and primary human HSPCs. CD109-KO or KD TF-1 cells underwent erythroid differentiation in the presence of TGF-β.

Sophoridine Derivatives Induce Apoptosis and Autophagy to Suppress the Growth of Triple-Negative Breast Cancer through Inhibition of mTOR Signaling.

In order to improve the antitumor potency and therapeutic margins of natural product sophoridine, its novel nitrogen mustard carbamate derivatives were designed and synthesized. In screening their in vitro activity, we found all the tested compounds were more potent against the highly aggressive triple-negative breast cancer cell line MDA-MB-231. Cellular functional assays showed that representative compounds could induce G1-phase arrest and trigger apoptosis, evidenced by the alteration of Bax, Bcl-2, caspase-3 and PARP levels.

Histone demethylase KDM6B inhibits breast cancer metastasis by regulating Wnt/β-catenin signaling.

Tumor metastasis remains a major challenge for patients with breast cancer. Aberrant epigenetic factor lysine-specific demethylase 6B (KDM6B) has been associated with tumor progression. Here, we show that KDM6B is significantly down-regulated in human breast cancer tissues, and its low expression is associated with poor prognosis of patients with breast cancer.

GP96 Drives Exacerbation of Secondary Bacterial Pneumonia following Influenza A Virus Infection.

Influenza A virus (IAV) infection predisposes the host to secondary bacterial pneumonia, known as a major cause of morbidity and mortality during influenza virus epidemics. Analysis of interactions between IAV-infected human epithelial cells and Streptococcus pneumoniae revealed that infected cells ectopically exhibited the endoplasmic reticulum chaperone glycoprotein 96 (GP96) on the surface. Importantly, efficient pneumococcal adherence to epithelial cells was imparted by interactions with extracellular GP96 and integrin α, with the surface expression mediated by GP96 chaperone activity.

Hyalase-Mediated Cascade Degradation of a Matrix Barrier and Immune Cell Penetration by a Photothermal Microneedle for Efficient Anticancer Therapy.

For melanoma with high lethality and metastasis rate, traditional therapy has limited effects; local photothermal therapy (PTT) synergetic with immune therapy for cancer treatment can perhaps improve the situation. However, because of the natural existence of the tumor matrix barrier, the penetration depth of drugs and immune cells often dampens the efficacy of cancer treatment. Herein, we report an innovative synergetic PTT and immune therapy through dissolving microneedles for the codelivery of the hyaluronidase-modified semiconductor polymer nanoparticles containing poly(cyclopentadithiophene--benzothiadiazole) and immune adjuvant polyinosinic-polycytidylic acid (PIC).

[A loss-of-function mutation in exon1 of limited HLA class I alleles is common in patients with aplastic anemia].

Leukocytes that lack HLA allelic expression (HLA-LLs) caused by a copy-number-neutral loss of heterozygosity in the short arm of chromosome 6 (6pLOH) and somatic mutations in HLA class I genes are commonly identified in patients with acquired aplastic anemia (AA), although the exact mechanisms underlying the HLA loss and HLA class I allele repertoire likely to acquire loss-of-function mutations remain unknown due to the limited number of AA patients that have been studied for loss-of-function mutations in HLA class I genes. We identified a common nonsense mutation at codon19 (c.19C>T, p.

[An Evaluation of Radiation Dose for Wide Volume Scan during Chest CT].

Purpose: A volume scan can cover a range of 160 mm with a single gantry rotation. It can be performed sequentially (a wide volume [WV] scan) to cover more than 160 mm, and volume Xact+ (Xact+) can be used when volume scan is done to extend the reconstruction area. The purpose of this study was to investigate the dose distribution and organ doses for a WV scan during chest CT.

Patient arm position during quantitative bone single-photon emission computed tomography/computed tomography acquisition can affect image quality and quantitative accuracy: a phantom study.

Purpose: The present study used a phantom to determine the effects of various arm positions on bone SPECT/computed tomography (CT) images and the optimal arm position to acquire good-quality and quantitatively accurate images.

Materials And Methods: We designed a phantom study of five simulated arm positions that are assumed during SPECT image acquisition. All SPECT data were acquired during a total of 120 projections of 10 and 100 s/view over 360° in a non-circular mode and reconstructed using Flash 3D (Siemens Healthineers).

Eltrombopag in Combination with Rabbit Anti-thymocyte Globulin/Cyclosporine A in Immunosuppressive Therapy-naïve Patients with Aplastic Anemia in Japan.

Objective In Japan, immunosuppressive therapy (IST) with anti-thymocyte globulin (ATG), and cyclosporine A (CsA) is the standard of care in patients with aplastic anemia (AA) who are not indicated for stem-cell transplantation, although some patients may experience relapse. This study assessed the efficacy and safety of eltrombopag in combination with rabbit-ATG/CsA in IST-naïve patients with non-severe or severe AA in Japan. Methods In this non-randomized, open-label, single-arm, phase II study, rabbit-ATG/CsA and eltrombopag were initiated on Days 1 and 15 (±3 days), respectively, and continued for ≥26 weeks; rabbit-ATG was given for 5 days (Days 1 to 5).

Associations of adverse pregnancy outcomes with high ambient air pollution exposure: Results from the Project ELEFANT.

Background: Investigations on the potential effects of high air pollution exposure before pregnancy on adverse pregnancy outcomes are limited, and it is unknown whether air quality standards looser than that set by World Health Organization (WHO) still can provide sufficient protection pregnant women from adverse pregnancy outcomes.

Objectives: To evaluate the potential effects of high ambient air pollution around pregnancy on preterm birth (PTB) and low birth weight (LBW), and assess the risk of PTB and LBW associated with air pollutants with reference to different air quality standards of WHO and China.

Methods: Our study leveraged 10,960 pregnant women from the Project ELEFANT.

Development of a computational phantom for validation of automated noise measurement in CT images.

The purpose of this study was to develop a computational phantom for validation of automatic noise calculations applied to all parts of the body, to investigate kernel size in determining noise, and to validate the accuracy of automatic noise calculation for several noise levels. The phantom consisted of objects with a very wide range of HU values, from -1000 to +950. The incremental value for each object was 10 HU.

Prenatal PM exposure and the risk of adverse births outcomes: Results from Project ELEFANT.

Background: Studies investigating the impact of fine particulate matter (PM) exposure during pregnancy upon adverse birth outcomes have primarily been performed in Western nations with low ambient PM levels. We examined associations between high levels of PM exposure during pregnancy and risk of adverse birth outcomes by timing and level of exposure in a Chinese population.

Methods: We analysed data from 10,738 live births within the Project ELEFANT study based in Tianjin, China.

Results from multinational phase 3 studies of ravulizumab (ALXN1210) versus eculizumab in adults with paroxysmal nocturnal hemoglobinuria: subgroup analysis of Japanese patients.

Ravulizumab demonstrated noninferior efficacy and comparable safety to eculizumab in two open-label, phase 3 studies in patients with paroxysmal nocturnal hemoglobinuria (PNH) who complement inhibitor-naive (Study 301) or were previously treated with eculizumab (Study 302). This subgroup analysis assessed ravulizumab's efficacy and safety in Japanese patients in Studies 301 and 302, who are known to have different clinicopathologic features from white patients. Patients were randomly assigned (1:1) to eculizumab every-two-weeks or weight-based dosing of ravulizumab every-eight-weeks for 26 weeks.

Resolution of Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis associated with rapid immune reconstruction after a single course of CHOP therapy.

Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis (EBV-HLH) is a life-threatening disease characterized by the uncontrolled proliferation of EBV-infected T/NK cells with resultant immune system failure against EBV. While a CD5HLA-DRCD8 T-cell population was previously shown to be EBV-infected cells and a useful marker for monitoring the response to treatment of EBV-HLH, changes in other lymphocyte subsets associated with EBV-HLH treatments have not been closely studied. We herein report a 25-year-old woman with EBV-HLH who presented with a fever, liver failure, and pancytopenia.

Phantom and clinical evaluation of bone SPECT/CT image reconstruction with xSPECT algorithm.

Background: Two novel methods of image reconstruction, xSPECT Quant (xQ) and xSPECT Bone (xB), that use an ordered subset conjugate gradient minimizer (OSCGM) for SPECT/CT reconstruction have been proposed. The present study compares the performance characteristics of xQ, xB, and conventional Flash3D (F3D) reconstruction using images derived from phantoms and patients.

Methods: A custom-designed body phantom for bone SPECT was scanned using a Symbia Intevo (Siemens Healthineers), and reconstructed xSPECT images were evaluated.

Identification of the Uptake Transporter Responsible for Distribution of Acotiamide into Stomach Tissue.

The acetylcholinesterase inhibitor, acotiamide, improves gastric motility and is clinically used to treat functional dyspepsia. The present study aimed to identify the transporters involved in the distribution of acotiamide in stomach tissue. Acotiamide uptake by the gastric cancer-derived model cell line, Hs746 T, was Na- and pH-independent.