Sustainable Removal of Tolperisone from Waters by Application of Photocatalysis, Nanotechnology, and Chemometrics: Quantification, Environmental Toxicity, and Degradation Optimization.

Environmental pollution is an emerging global issue. Heterogenous photocatalytic degradation, which belongs to the advanced oxidation processes, is a promising sustainable technique for the removal of harmful pollutants (e.g.

New insight into the perplexing toxic features of PCBs: A study of nephrotoxicity in an animal model.

The present study investigated the effects of PCBs on the rat kidneys with attention given to the determination critical effect dose (CED) using the Benchmark dose (BMD) approach. Male albino Wistar rats (7 animals per group) were given by oral gavage Aroclor 1254 dissolved in corn oil at doses of 0.0, 0.

Cadmium dietary exposure assessment in the adult population and pre-school children in the Republic of Serbia.

Cadmium (Cd) is a toxic metal, present in all matrices of the environment and a common food contaminant. Human exposure to it may elicit many diverse health impairments. The aim of this study was to assess the dietary exposure to Cd for the adult population and preschool children in Serbia using probabilistic methodology.

Cadmium, lead, mercury, and arsenic in fresh fruits and fruit products intended for human consumption in the Republic of Serbia, 2015-2017.

The European Commission has established legislation for toxic metals in certain foodstuffs in order to protect public health already in 1993. After several amendments, new maximum levels for cadmium and lead in certain foods were set as per 30 and 31 August 2021 for lead and cadmium, respectively. The new Serbian Regulation on "maximum levels of certain contaminants in food" is fully harmonised with this European legislation.

ST2 and the alteration of cobalt, sodium, potassium and calcium concentration in acute inflammation.

Introduction: ST2 is the receptor for interleukin (IL)-33, the last discovered member of the IL-1 cytokine family. Acute inflammation is an early response of vascularized tissue to injury, in which alteration of micro- and macro-elements occurs. This study aimed to examine the alteration of cobalt, sodium, potassium, and calcium concentration at the site of acute inflammation and the role of ST2 in these alterations.

Seroprevalence, Direct Detection and Risk Factors for Infection in Pigs in Serbia, and Influence of Biosecurity Measures.

Consumption of contaminated pork is a major risk factor for human infection. We thus conducted a cross-sectional survey on the seroprevalence of infection in a representative sample of slaughter pigs from throughout Serbia and examined the influence of farm biosecurity-related risk factors on infection. In addition, direct detection of the parasite (by mouse bioassay) or its DNA was performed in the hearts of a subset of seropositive sows.

Redesigned pMAL expression vector for easy and fast purification of active native antimicrobial peptides.

Aims: The aim of this study was to construct the improved pMAL expression vector to increase the efficacy of purification of small native peptides and their clear-cut separation from MBP tag. The modifications we introduced can be applied to many expression vectors.

Methods And Results: To improve the pMAL expression vector, we introduced the His tag and the enterokinase cleavage site (Ek) downstream from the MBP tag and Xa cleavage site on the original vector.

Determination of antilisterial effect of some microbial isolates from traditional during the fermentation of soft white cheese.

The aim of this study was to select autochthonous lactic acid bacteria (LAB) isolates with antilisterial activity from Zlatar cheese and to evaluate the ability of selected LAB to control growth during soft white cheese production. The genotype characterization of isolated LAB (n = 93) was done using PCR method by 16S rRNA sequencing. In this way, the following isolates were detected: ssp.

Quality Multiverse of Beef and Pork Meat in a Single Score.

The rationale behind this review is the potential of developing a single score tool for meat quality evaluation based on visual and sensorial assessments of fresh meat. Based on the known sensory wheel concept, the first step was to create quality wheels capturing most common intrinsic and extrinsic quality cues of pork and beef outlined in the latest scientific papers. This resulted in identifying meat color, sensory characteristics and fat content as the most important intrinsic quality cues of fresh beef and pork.

Reformulation of Traditional Fermented Sausage Utilizing Novel (Digital) Methods of Analysis.

The main objective of this paper was to investigate the effect of fat reduction on different quality traits of sausage. This study also aimed to deploy the following digital methods of analysis: three-dimensional (3D) laser imaging, computer vision system and oral processing. Three batches of sausage with different amounts of pork back fat were manufactured: control (25%), medium fat (17.

Ochratoxin A potentiates citrinin accumulation in kidney and liver of rats.

Ochratoxin A (OTA) and citrinin (CTN) are nephrotoxic mycotoxins often found together in grain. The aim of this study was to measure their accumulation in the kidney and liver of adult male Wistar rats, see how it would be affected by combined treatment, and to determine if resveratrol (RSV) would decrease their levels in these organs. The rats received 125 or 250 mg/kg bw of OTA by gavage every day for 21 days and/or 20 mg/kg bw of CTN a day for two days.

Prevalence, characterization, and antibiotic susceptibility of spp. in a milk powder processing environment: The first reported case in Serbia.

spp. are opportunistic foodborne pathogens that most often infect neonates and infants through contaminated powdered infant formula. No reports have been published in Serbia on the prevalence of spp.

Landscape of Stress Response and Virulence Genes Among Strains.

The pathogenic microorganism is ubiquitous and responsible for listeriosis, a disease with a high mortality rate in susceptible people. It can persist in different habitats, including the farm environment, the food production environments, and in foods. This pathogen can grow under challenging conditions, such as low pH, low temperatures, and high salt concentrations.

The Intake of Phosphorus and Nitrites through Meat Products: A Health Risk Assessment of Children Aged 1 to 9 Years Old in Serbia.

This study provides the data on dietary exposure of Serbian children to nitrites and phosphorus from meat products by combining individual consumption data with available analytical data of meat products. A total of 2603 and 1900 commercially available meat products were categorized into seven groups and analysed for nitrite and phosphorous content. The highest mean levels of nitrite content, expressed as NaNO, were found in finely minced cooked sausages (40.

Dietary Exposure and Risk Assessment of Aflatoxin M1 for Children Aged 1 to 9 Years Old in Serbia.

The present study was conducted to estimate the exposure and characterize the risk for the child population of Serbia to Aflatoxin M1 (AFM1) from milk and milk-based food. A total of 3404 samples comprising milk and different milk-based food samples were collected from various regions of Serbia from 2017 to 2019. Evaluation of AFM1 exposure was carried out using the deterministic method, whereas risk characterization was evaluated using the margin of exposure (MOE) and the risk of hepatocellular carcinoma (HCC).

Human health risks and benefits assessment based on OCPs, PCBs, toxic elements and fatty acids in the pelagic fish species from the Adriatic Sea.

Benefits and risks of the fish consumption should be assessed to balance the ingestion of healthy omega-3 fatty acids and adverse chemicals. Persistent organic pollutants-POPs (organochlorine pesticides-OCPs and polychlorinated biphenyls-PCB), macro- and micro-elements and fatty acid contents were determined in six fish species from the Adriatic Sea to assess health risks for consumers (worst-case scenario, diseases development risks and benefit-risk). 16 element, 24 POPs and 14 fatty acid contents were determined in six pelagic species which can be used in human diet.

Characterisation of changes in physicochemical, textural and microbiological properties of sausage during ripening.

The purpose of this paper was to investigate effect of ripening time on physicochemical, textural and microbiological properties of (dry fermented sausage) during ripening and to conduct sensory and free fatty acid characterization of final product. Ripening time significantly ( < 0.05) affected all physicochemical parameters.

Fatty acids, persistent organic pollutants, and trace elements in small pelagic fish from the eastern Mediterranean Sea.

Fatty acids (FAs) composition, 24 persistent organic pollutants (POPs), and 16 trace elements were examined in small pelagic fish (sardine, anchovy, round sardinella, chub and horse mackerels) caught by a fishing fleet for more than three years in the eastern Mediterranean Sea. Five Unmix source profiles associated with both sources, such as overlapping diet, including low-niche marine organisms and inputs from the surrounding environmental compartments were resolved. Inorganic compounds were notably more abundant in fish tissue than organochlorine xenobiotics.

Determination of toxic elements in meat products from Serbia packaged in tinplate cans.

This work aimed to examine the influence of the storage period on the content of toxic elements (As, Cd, Hg, and Pb) in five types of canned meat products regularly used in the Serbian Armed Forces. Cans of beef goulash (BG), pork ragout (PR), spam (SP), liver pate (LP), and meatballs in tomato sauce (MB), produced according to military standards and stored under regular conditions, were analyzed. Meat products were packed in tin cans made according to special requirements in terms of tin and varnish application and stored for up to 6 years.

Age-specific accumulation of toxic metal(loid)s in northern pike (Esox lucius) juveniles.

Northern pike specimens of 0, 1, and 2 age classes were collected in June 2013 from the Vizelj channel near Belgrade (Serbia). Inductively coupled plasma optical spectrometry (ICP-OES) and atomic absorption spectrometer SpectrAA 220 were used for analyzing concentrations of As, Cr, Cd, Co, Cu, Fe, Sr, Pb, and Zn, as well as Hg in muscle, gills, and liver. Metal pollution index (MPI) was calculated.

Tracking the Origin of Austrian Human Brucellosis Cases Using Whole Genome Sequencing.

Brucellosis is a zoonotic disease caused by spp. and a major concern for livestock. Most human cases are caused by and clinical presentation is usually a mild febrile illness.