[Morphometric study of yeast cells of Torulopsis sphaerica after He-Ne-laser irradiation].

A study was made of structural organization of Torulopsis sphaerica cells irradiated with He-Ne (lambda = 632.8 nm; dose--460 J/m2) and then cultured in the nutrient with 1% glucose and O2 for 6 h. The computer analysis of electron images of cell sections was carried out.

Melatonin modulates the action of near infrared radiation on cell adhesion.

The adhesion of human cervical cancer (HeLa) cells to a glass matrix is evaluated following their irradiation in a suspension with a pulsed near-infrared (IR) light-emitting diode (wavelength 820 nm, pulse repetition frequency 10 Hz, irradiation dose 16-120 J/m2) when melatonin (4 x 10(-11) to 4 x 10(-5) m) is added to cell suspension immediately before or after the irradiation. Also, the dependence of visible-to-near-IR radiation (600-840 nm, 52 J/m2) on cell adhesion (action spectrum) is recorded in absence and presence of melatonin (4 x 10(-6) m). It is found that melatonin in pharmacological concentrations (but not in physiological range) inhibited cell adherence.

Cell attachment to extracellular matrices is modulated by pulsed radiation at 820 nm and chemicals that modify the activity of enzymes in the plasma membrane.

Background And Objectives: Adhesive interactions between cells and extracellular matrices play a regulative role in wound repair processes. The objective of this investigation is to study action mechanisms of pulsed radiation at 820 nm on cellular adhesion in vitro. Light emitting diodes (LED) at 820 nm are widely used for treatment of wounds of various etiology.

Donors of NO and pulsed radiation at lambda = 820 nm exert effects on cell attachment to extracellular matrices.

The adhesion of HeLa cells to a glass matrix was evaluated after the irradiation of the cell suspension with a pulsed near-infrared light-emitting diode (lambda = 820 nm, frequency 10 Hz, dose 8-120 J/m(2)) and treatment with two donors of nitric oxide, sodium nitroprusside (SNP, 5 x 10(-4) M) and NaNO(2) (4 x 10(-4) M). It was found that in an irradiated cell suspension, the cell-glass adhesion increases in a dose-dependent manner (a bell-shaped curve with a maximum at 60 J/m(2)). The treatment of cells with SNP or NaNO(2) before the irradiation eliminates the radiation-induced attachment stimulation.

Cell attachment modulation by radiation from a pulsed light diode (lambda = 820 nm) and various chemicals.

Background And Objective: Adhesive interactions between cells and extracellular matrices play a regulative role in wound repair processes. The objective of this investigation is to study the mechanisms of light action on cellular adhesion in vitro. The adhesion of HeLa cells to a glass matrix is evaluated after irradiation with a pulsed near-infrared (IR) diode and treatment with various chemicals.