Impact of aspirate smears and trephine biopsies in routine bone marrow diagnostics: a comparative study of 141 cases.

The diagnostic impact of bone marrow cytology in combination with flow cytometry analysis of aspirate smears and bone marrow histology together with immunohistochemical examination of trephine biopsies was compared in 141 routine cases. Diagnoses achieved by the two methods were concordant in 80.5% of cases.

Anti-thyroid peroxidase (anti-TPO) antibodies in thyroid diseases, non-thyroidal illness and controls. Clinical validity of a new commercial method for detection of anti-TPO (thyroid microsomal) autoantibodies.

The identification of the thyroid peroxidase (TPO) as the main antigen of the thyroid microsomal fraction has enabled the development of a sensitive and specific assay for detection of the corresponding autoantibodies. We evaluated the diagnostic validity of the anti-TPO assay in 303 patients with different types of thyroid disease and in controls. Clearly elevated anti-TPO values (anti-TPO > 500 units/ml) were found in 59% of patients with thyroiditis but in none of the controls or the patients with non-thyroidal illness.

Blood substitutes made on the basis of perfluorocarbons inhibit intracellular energy generation.

Although perfluorocarbons (PFC) are chemically inert, toxic reactions are observed on using them as fluorocarbon emulsions in blood substitutes. Six to twelve hours after exchanging about half of the circulating blood of conscious rats pathobiochemical reactions occur despite a high interarterial oxygen pressure. They indicate the disturbance of intracellular energy generation, which is characterized by a decrease in ATP, increase in ADP, inorganic phosphate and potassium, increase in NADH and lactate.

Beta 2-microglobulins and renal dysfunction in burned patients.

Studies have been made of the serum and urinary beta-microglobulin levels in 18 burns patients in order to estimate glomerular and proximal tubular dysfunction. The greatest urinary excretion of beta 2-microglobulin was found in severely burned patients during the second and third weeks after injury. Urinary measurements of this protein in patients with renal tubular dysfunction have proved to be of value in the assessment of renal tubular damage.