[Thermodynamic outlook and practice of Chinese medicinal nature].

Medicinal nature theory of Chinese medicine is the difficult and hot issue in the basic research of Chinese medicine (CM), but has not yet obtained some important breakthrough until now. The cold and heat syndromes is considered as the capital differentiation of CM in clinic; cold and hot is the primary medicinal nature of CM. Treating the cold with heat, the heat with cold is the main therapeutic principle of CM.

Epigallocatechin gallate protects H9c2 cardiomyoblasts against hydrogen dioxides- induced apoptosis and telomere attrition.

Epigallocatechin gallate (EGCG), the major component of polyphenols in green tea, has recently attracted considerable attention for its cardioprotective effects. Telomere signalling plays a role in regulating cardiomyocyte apoptosis during cardiac dysfunction. The purpose of this study was to investigate the effects of EGCG on oxidative stress-induced apoptosis and telomere attrition in cardiomyocytes.

Investigation of the differences between the "Cold" and "Hot" nature of Coptis chinensis Franch and its processed materials based on animal's temperature tropism.

The description and differentiation of the so-called "Cold" and "Hot" natures, the primary "Drug Naure" of Chinese medicine, is the focus of theoretical research. In this study, the divergency between the "Cold" and the "Hot" natures was investigated through examining the temperature tropism of mice affected by Coptis chinensis Franch and its processed materials by using a cold/hot plate differentiating technology. After exposure to C.

[Survey of investigations on Daodi Chinese medicinal materials in China since 1980s].

In the recent 20 years, three major investigational achievements about Daodi CMM (Chinese medicinal materials) have been made as follows: (1) The herbal textual about Daodi CMM have been collected and revised systematically, and the causes and pattern of Daodi CMM formation and development were illustrated. (2) A series of appraising methods and marks for Daodi CMM have been set up initially, the scientific connotation of Daodi CMM has been clarified primarily, which promotes the development of pharmacognosy discipline consequently. (3) Based on the Daodi CMM research program, the GAP of CMM with good quality, high yield and best benefit has been carried out elementarily, which is helpful to solve some economic problems and troubles which the peasant, countryside and agriculture always worry about.

[Some key issues about scientific research on traditional Chinese medicine].

Since the Tenth Five-Year Plan in China, the science and technology of traditional Chinese medicine (TCM) has gained unprecedented high attention, and at the same time, the people who have been engaging in the TCM research are bearing more and more missions and responsibilities. In order to get more significant breakthroughs and scientific achievements with great innovation, great value and great influence in the near future, the radical target and strategy for TCM research should be made out as follows: sifting out the lees, laying aside the disputes, tamping the identical opinions, and innovating to apply. In other words, the principle that "to do what can be done, not to do what can not be done; to do what should be done, not to do what should not be done, and to pay more attention to standardization than to innovation" should be recognized unequivocally.

[Effect of environmental factors on growth of Huperzia serrata].

Objective: To establish and optimize the cultivation systems for wild Huperzia serrata, through assessing the effects of environmental factors on its growth.

Method: H. serrata was planted under natural field condition, the growth status of H.

[Establishment and application of AFLP fingerprinting system in Atractylodes macrocephala germplasm].

Objective: To establish the AFLP Fingerprinting system in the germplasm of Atractylodes macrocephala Koidz.

Methods: 10 wild or cultivated Atractylodes macrocephala were used for AFLP fingerprinting analysis by EcoRI and MseI restriction enzymes with silver staining.

Results: Using 2X CTAB buffer extraction method can obtain the best genomic DNA samples.

[Effect of extracted ZG from gardenia on Hep-2 cell membrane post infected with parainfluenza virus type 1 (PIV-1)].

In order to study the anti-viral mechanism of extracted ZG from Gardenia, the effect of extracted ZG on Hep-2 cell membrane potential, Na -K+-ATPase activity and membrane fluidity post infected with parainfluenza virus type 1 (PIV-1) was observed. Acetylcholine which was fluorescent labeled with DiBAC4 (3) was taken as positive control to observe the changes of membrane potential and was measured by flow cytometer. The phosphorus determination method and spectrophotometer were used to measure the Na+-K+-ATPase activity of Hep-2 cell membrane post PIV-1 infection.

[Probe into innovation and development of pattern of quality control and evaluation for Chinese medicine].

To set up a new pattern of quality control and evaluation for Chinese medicine. By investigating the limitation of quality control pattern for Chinese medicine, the differences and similarities in the chemical substantial style as well as quality control patterns among Chinese medicine, chemical synthetic drugs and Biologicals, combining with the author's experience on the research of geo-authentic medicinal material and theory of Chinese medicinal nature, a new pattern of quality control for Chinese medicine has been explored and designed. A more rational pattern of quality control for Chinese medicine should be referred to Biologicals instead of chemical synthetic drugs, there are more similarity in chemical substantial style and quality control pattern for Chinese medicine between Chinese medicine and Biologicals than that between Chinese medicine and chemical synthetic drugs.

[Study on preparation technology of plumula nelumbinis Alkaloid Dripping Pill and determination of content by HPLC].

Objective: To determine the optimum technical parameters through controlling the different factors and the content of Dripping Pill.

Method: Time of dissolution, spherical degree and pill weight as parameters, the influential factors were investigated by orthogonal test and then to determine the content of liensinine, isoliensinine, neferine in plumula nelumbinis Alkaloid Dripping Pill.

Result: The optimum condition: 75% PEG4000 as matrix, methyl-silicon oil as refrigerant, 4 cm dripping distance, 10 degrees C refrigerant, 30 drippings per minute.

[Antivirus effects of extract from gardenia].

Objective: To observe the effect of the extract from gardenia on influenza viral pneumonia in mice and virus-induced cytopathic effect.

Method: The mice were infected by influenza virus in nasal, the lung inflammation, mortality rate and life elongation rate were observed respectively. The anti-viral activity of the extract from gardenia was accessed by cytopathic effect (CPE) in vitro and 0% toxicity concentration (TC0), 50% toxicity concentration (TC50), 50% inhibitor concentration (IC50), therapeutic index (TI) were determined by Reed-Muench method.

[Study on screening immunocopetent position in Rabdosia amethystoieds (Benth.) Hara].

Objective: To search for immunocompetent position in Rabdosia amethystoieds (Benth.) Hara.

Methods: Spleen cells of mice were stimulated by mitogen concannvalin A (ConA), and proliferation of lymphocytes were measured with tetrazolium salts (MTT) in vitro.

[Study on complex system of Chinese materia medica GAP fulfilling].

Objective: To provide new model of thinking and management for Chinese Meteria Medica GAP fulfilling.

Method: After analyzing the problems of GAP fulfilling, and considering GAP a complex system, we had some initial discussions about the systematic structure, characteristics and the methods for fulfilling it.

Result: The GAP was a complex system which consisted of biological system, environment system and management system with its own characteristics and methods.

[HPLC determination of 6-gingerol in Rhizoma Zingiberis Recens].

Objective: To determine the contents of 6-Gingerol in Rhizoma Zingiberis Recens.

Method: HPLC method was used, with Alltech C18 column, acetonitrile-methol-water (43:5:52) as mobile phase with a flow rate of 0.8 mL.

[Cultivation of Pinella ternata in artificial soil and its growth habits].

In order to increase yield, the cultivation of Pinellia ternata in artificial soil was studied, and its growth habits was investigated. At same time, the measures of dominating soil thickness, using nutrient solution as fertillizer and complete illumination were taken. The research results can supply a reference for the industrial production of P.

[Consideration on the problem of "ascension of the case poisoned by Chinese Traditional Medicines"].

Objective: Desserting toxicity and reasonable usage of Chinese traditional medicines.

Methods: Analysing the understanding of toxicity of Chinese traditional medicines in Chinese medicine and pharmacology and the reason for ascension of the case poisoned by Chinese traditional medicines.

Results And Conclusion: All medicines are poisonous.

[Chemical constituents of Notopterygium incisium Ting. III: Chemical constituents isolated and identified from petroleum ether extracts of N. incisium Ting].

Six chemical compounds were isolated from the roots and rhizoma of Notopterygium incisium by column chromatography and preparative thin-layer chromatography, and identified by chemical and spectroscopic analysis as beta-sitosterol, oleic acid, linoleic acid, columbianetin, pregnenolone and ferulic acid. The presence of pregnenolone in the genus Notopterygium has not been reported.