65 results match your criteria: "Institute of Cellular Biology and Pathology "N. Simionescu"[Affiliation]"
J Transl Med
November 2024
Department of Translational Medicine, Lund University, Malmö, Sweden.
Introduction: The role of the complement inhibitory proteins CD46 and CD59 in the immune response to an acute coronary syndrome (ACS) is unknown. We investigated the relationships between the shedding of CD46 and CD59 into the circulation, reflected by plasma levels of soluble CD46 and CD59, and the risk for post-ACS complications.
Methods: We measured plasma sCD46 and sCD59 in a cohort of 546 ACS patients within 24 h after hospital admission, and after 6-weeks in a subgroup of 114 patients.
Pharmacol Rep
August 2022
Institute of Cellular Biology and Pathology "N. Simionescu", Bucharest, Romania.
Background: Apolipoprotein E (apoE) is an anti-atherosclerotic protein associated with almost all plasma lipoproteins. Fullerenol (Full-OH) contains the fullerene hydrophobic cage and several hydroxyl groups that could be derivatized to covalently bind various molecules. Herein, we aimed to produce fullerenol-based nanoparticles carrying apoE3 (Full-apoE) and test their anti-atherosclerotic effects.
View Article and Find Full Text PDFInt J Mol Sci
May 2022
Department of Proteomics, Institute of Cellular Biology and Pathology "N. Simionescu" of the Romanian Academy, 050568 Bucharest, Romania.
Immunogenetics
October 2022
Cantacuzino Military-Medical Research and Development National Institute, Bucharest, Romania.
We herein analyzed all available protein-protein interfaces of the immune complexes from the Protein Data Bank whose antigens belong to pathogens or cancers that are modulated by fever in mammalian hosts. We also included, for comparison, protein interfaces from immune complexes that are not significantly modulated by the fever response. We highlight the distribution of amino acids at these viral, bacterial, protozoan and cancer epitopes, and at their corresponding paratopes that belong strictly to monoclonal antibodies.
View Article and Find Full Text PDFInt J Mol Sci
July 2021
Department 4-Cardiothoracic Pathology, University of Medicine and Pharmacy Carol Davila, 8, Eroii Sanitari Bvd., 050474 Bucharest, Romania.
Int J Mol Sci
January 2021
Institute of Cellular Biology and Pathology "N. Simionescu", 8, B.P. Hasdeu Street, 050568 Bucharest, Romania.
Adenoviral vectors are important vehicles for delivering therapeutic genes into mammalian cells. However, the yield of the adenoviral transduction of murine mesenchymal stromal cells (MSC) is low. Here, we aimed to improve the adenoviral transduction efficiency of bone marrow-derived MSC.
View Article and Find Full Text PDFSci Rep
October 2020
Institute of Cellular Biology and Pathology "N. Simionescu" of the Romanian Academy, 8, B.P. Hasdeu Street, PO Box 35-14, 050568, Bucharest, Romania.
Due to their excellent mechanical and biocompatibility properties, titanium-based implants are successfully used as biomedical devices. However, when new bone formation fails for different reasons, impaired fracture healing becomes a clinical problem and affects the patient's quality of life. We aimed to design a new bioactive surface of titanium implants with a synergetic PEG biopolymer-based composition for gradual delivery of growth factors (FGF2, VEGF, and BMP4) during bone healing.
View Article and Find Full Text PDFInt J Mol Sci
August 2020
Gene Regulation and Molecular Therapies Laboratory, Institute of Cellular Biology and Pathology "N. Simionescu", 8, B.P. Hasdeu Street, 050568 Bucharest, Romania.
Fas ligand (First apoptosis signal ligand, FasL, also known as CD95L) is the common executioner of apoptosis within the tumor necrosis factor (TNF) superfamily. We aimed to induce functional FasL expression in transduced cells using an adenovirus vector, which has the advantage of strong and transient induction of the gene included in the adenoviral genome. Here, we report that the adenovirus carrying a truncated gene, named minigene, encoding the full-length FasL protein (Ad-gFasL) is more efficient than the adenovirus carrying FasL cDNA (Ad-cFasL) in the induction of FasL expression in transduced cells.
View Article and Find Full Text PDFExpert Rev Proteomics
April 2020
Ephraim Katzir Department of Biotechnology Engineering, ORT Braude College, Karmiel, Israel.
Introduction: The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Biomarkers have taken a central position either as companion markers to support drug development and patient selection, or as indicators aiming to detect the earliest perturbations indicative of disease, minimizing therapeutic intervention or even enabling disease reversal. Protein biomarkers are of particular interest given their central role in biochemical pathways.
View Article and Find Full Text PDFPolymers (Basel)
March 2020
Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, Magurele, 077190 Ilfov, Romania.
Our study focused on the long-term degradation under simulated conditions of coatings based on different compositions of polycaprolactone-polyethylene glycol blends (PCL-blend-PEG), fabricated for titanium implants by a dip-coating technique. The degradation behavior of polymeric coatings was evaluated by polymer mass loss measurements of the PCL-blend-PEG during immersion in SBF up to 16 weeks and correlated with those yielded from electrochemical experiments. The results are thoroughly supported by extensive compositional and surface analyses (FTIR, GIXRD, SEM, and wettability investigations).
View Article and Find Full Text PDFCell Tissue Res
January 2020
Institute of Cellular Biology and Pathology "N. Simionescu", 8, B.P. Hasdeu Street, P.O. Box 35-14, Bucharest, Romania.
Int J Mol Sci
November 2019
Institute of Cellular Biology and Pathology "N. Simionescu", 050568 Bucharest, Romania.
Apolipoprotein C1 (apoC1), the smallest of all apolipoproteins, participates in lipid transport and metabolism. In humans, gene is in linkage disequilibrium with gene on chromosome 19, a proximity that spurred its investigation. Apolipoprotein C1 associates with triglyceride-rich lipoproteins and HDL and exchanges between lipoprotein classes.
View Article and Find Full Text PDFJ Cell Mol Med
November 2019
Department of Regenerative Medicine, "N. Simionescu" Institute of Cell Biology and Pathology, Bucharest, Romania.
The aim of the study was to evidence replicative senescence-induced changes in human amniocytes via flow cytometry, quantitative reverse-transcription-polymerase chain reaction (qRT-PCR) and automated/manual patch-clamp. Both cryopreserved and senescent amniocytes cultured in BIO-AMF-2 medium featured high percentages of pluripotency cell surface antigens SSEA-1, SSEA-4, TRA1-60, TRA1-81 (assessed by flow cytometry) and expression of pluripotency markers Oct4 (Pou5f1) and Nanog (by qRT-PCR). We demonstrated in senescent vs cryopreserved amniocytes decreases in mesenchymal stem cell surface markers.
View Article and Find Full Text PDFInt J Mol Sci
March 2019
Institute of Cellular Biology and Pathology "N. Simionescu", 050568 Bucharest, Romania.
Apolipoprotein E (apoE) is mainly secreted by hepatocytes and incorporated into most plasma lipoproteins. Macrophages, which accumulate cholesterol and are critical for the development of the atherosclerotic plaque, are also an important, albeit smaller, apoE source. Distal regulatory elements control cell-specific activity of the apoE promoter: multienhancers (ME.
View Article and Find Full Text PDFInt J Pharm
April 2017
National Institute for Lasers, Plasma & Radiation Physics, Lasers Department, P.O. Box MG-36, Bucharest-Magurele, Bucharest 769231, Romania. Electronic address:
The purpose of this study was to obtain, characterize and evaluate the cytotoxicity and antimicrobial activity of coatings based on poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) - Lysozyme (P(3HB-3HV)/Lys) and P(3HB-3HV) - Polyethylene glycol - Lysozyme (P(3HB-3HV)/PEG/Lys) spheres prepared by Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique, in order to obtain functional and improved Ti-based implants. Morphological investigation of the coatings by Infrared Microscopy (IRM) and SEM revealed that the average diameter of P(3HB-3HV)/Lys spheres is around 2μm and unlike the drop cast samples, IRM recorded on MAPLE films revealed a good distribution of monitored functional groups on the entire scanned surface. The biological evaluation of MAPLE structured surfaces revealed an improved biocompatibility with respect to osteoblasts and endothelial cells as compared with Ti substrates and an enhanced anti-biofilm effect against Gram positive (Staphylococcus aureus) and Gram negative (Pseudomonas aeruginosa) tested strains.
View Article and Find Full Text PDFJ Proteomics
February 2017
Institute of Cellular Biology and Pathology "N. Simionescu" of the Romanian Academy, Bucharest, Romania. Electronic address:
Unlabelled: There is a wide range of pathological conditions proved to be associated with inflammation. The inflammatory process offers protection against harmful stimuli such as induced cell injury and tissues damage by means of specialized mediators and cells. Alarmins, also known as endogenous danger signals or damage-associated molecular patterns (DAMPs) molecules, are critical players of immune response to tissue suffering.
View Article and Find Full Text PDFBraz J Med Biol Res
January 2017
Department of Physiology, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania.
Supraphysiological administration of anabolic androgenic steroids has been linked to increased blood pressure. The widely distributed amino acid taurine seems to be an effective depressor agent in drug-induced hypertension. The purpose of this study was to assess the impact of chronic high dose administration of nandrolone decanoate (DECA) and taurine on blood pressure in rats and to verify the potentially involved mechanisms.
View Article and Find Full Text PDFBiochim Biophys Acta
September 2016
Institute of Cellular Biology and Pathology "N. Simionescu", 8, BP Hasdeu Street, PO Box: 35-14, 050568 Bucharest, Romania. Electronic address:
J Physiol Sci
November 2016
Flow Cytometry and Cell Therapy Laboratory, "N. Simionescu" Institute of Cellular Biology and Pathology, B.P. Hasdeu Str. 8, 050568, Bucharest, Romania.
We characterized human amniotic fluid stem cells (AFSC) in senescent cultures (6 weeks) versus cryopreserved cells using whole-cell patch-clamp, immunophenotyping, and differential gene expression profiling for senescence genes. We evidenced five ion current components (outward rectifier, A-type, inward rectifier, and big conductance Ca-dependent K currents, fast voltage-dependent Na currents). Senescent AFSC showed reduced expression of CD90, CD44, CD133, over 500-fold increase of interferon gamma and telomerase reverse transcriptase genes, increased cycle-dependent kinase 4 inhibitors, p53-binding protein 1, and decreased calreticulin and CD44.
View Article and Find Full Text PDFJ Control Release
December 2015
Institute of Physiology, University of Zürich and Zürich Center for Integrative Human Physiology, CH-8057 Zurich, Switzerland. Electronic address:
Enhanced levels of the inflammatory chemokine CCL2 are known to correlate with increased tumorigenesis and metastases, and thereby poor prognosis for cancer patients. The CCL2-CCR2 chemokine axis was shown to facilitate the metastatic initiation through the recruitment of inflammatory monocytes and the activation of endothelial cells at metastatic sites. Both steps are required for efficient cancer cell trans-endothelial migration and seeding in the targeted tissue.
View Article and Find Full Text PDFCurr Stem Cell Res Ther
March 2017
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In modern society, myocardial infarction is a major cause of mortality, morbidity and deterioration of quality of life. Although various therapeutic approaches are available, none of them lead to the regeneration of infarcted tissue. The use of mesenchymal stem cells in cell therapy for myocardial infarction showed a beneficial effect consisting in reduced infarcted area and improved cardiac function, which can be explained by paracrine mechanism.
View Article and Find Full Text PDFJ Cell Mol Med
August 2015
Institute for Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University, Jerusalem, Israel.
Vascular endothelial cell (VEC) senescence is considered an early event in the development of atherosclerotic lesions. Stressful stimuli, in particular oxidative stress, have been linked to premature senescence in the vasculature. Foam cells are a major source of reactive oxygen species and may play a role in the induction of VEC senescence; hence, we investigated their involvement in the induction of VEC senescence in a co-culture transwell system.
View Article and Find Full Text PDFChemokines are critically involved in the development of chronic inflammatory-associated diseases such as atherosclerosis. We hypothesized that targeted delivery of compounds to the surface of activated endothelial cells (EC) interferes with chemokine/receptor interaction and thereby efficiently blocks inflammation. We developed PEGylated target-sensitive liposomes (TSL) encapsulating a CCR2 antagonist (Teijin compound 1) coupled with a specific peptide recognized by endothelial VCAM-1 (Vp-TSL-Tj).
View Article and Find Full Text PDFArch Physiol Biochem
October 2014
Institute of Cellular Biology and Pathology "N. Simionescu" of the Romanian Academy, 8, B.P. Hasdeu Street, Bucharest 050568 , Romania.
The interaction between antioxidant glutathione and the free thiol in susceptible cysteine residues of proteins leads to reversible protein S-glutathionylation. This reaction ensures cellular homeostasis control (as a common redox-dependent post-translational modification associated with signal transduction) and intervenes in oxidative stress-related cardiovascular pathology (as initiated by redox imbalance). The purpose of this review is to evaluate the recent knowledge on protein S-glutathionylation in terms of chemistry, broad cellular intervention, specific quantification, and potential for therapeutic exploitation.
View Article and Find Full Text PDFArch Physiol Biochem
December 2013
Institute of Cellular Biology and Pathology ''N. Simionescu'' of the Romanian Academy 8, B.P. Hasdeu Street, Bucharest 050568 , Romania.
Hydrogen sulphide (H(2)S) is the most recently discovered gasotransmitter. It is endogenously generated in mammalian vascular cells and attracts substantial interest by its function as physiological relevant signalling mediator, and by its dysfunction in metabolic diseases like obesity, type 2 diabetes and their associated complications. The purpose of this review is to highlight the novel findings on vascular H(2)S homeostasis, pathology-associated dysregulation, cell signalling, and therapeutic potential.
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