Validation of a Brief Physical Activity Assessment Tool for Adults With Limited Resources.

Objective: To validate a Brief Physical Activity Assessment Tool for the Expanded Food and Nutrition Program (EFNEP).

Design: Phase 1: test-retest reliability based on 2 survey administrations; Phase 2: concurrent validity triangulated with accelerometer data; Phase 3: sensitivity to change and responsiveness and secondary analysis of EFNEP national dataset.

Setting: Community centers and/or EFNEP classes across the US.

Genome-wide identification, characterization and expression analysis of the transcription factors in garlic ( L.).

Introduction: The genes () are essential in numerous biological processes, including development and stress responses. Despite extensive research on in many plants, a comprehensive genome-wide analysis of in garlic has yet to be undertaken.

Methods: In this study, we identified and classified 64 genes () into 10 subfamilies.

Structural insights into instability of the nucleosome driven by histone variant H3T.

The testis-specific histone variant H3T plays a crucial role in chromatin reorganization during spermatogenesis by destabilizing nucleosomes. However, the structure basis for the nucleosome instability driven by H3T is not fully understand. In this study, we determinate the crystal structure of H3T-H4 in complex with histone chaperone ASF1a at 2.

Structural studies of WDR5 in complex with MBD3C WIN motif reveal a unique binding mode.

The nucleosome remodeling and deacetylase (NuRD) complex plays a pivotal role in chromatin regulation and transcriptional repression. In mice, methyl-CpG binding domain 3 isoform C (MBD3C) interacts specifically with the histone H3 binding protein WD repeat-containing protein 5 (WDR5) and forms the WDR5-MBD3C/Norde complex. Despite the functional significance of this interaction on embryonic stem cell gene regulation, the molecular mechanism underlying MBD3C recognition by WDR5 remains elusive.

Genome-wide identification, classification and expression analysis of the heat shock transcription factor family in Garlic (Allium sativum L.).

Background: The heat shock transcription factor (HSF) plays a crucial role in the regulatory network by coordinating responses to heat stress as well as other stress signaling pathways. Despite extensive studies on HSF functions in various plant species, our understanding of this gene family in garlic, an important crop with nutritional and medicinal value, remains limited. In this study, we conducted a comprehensive investigation of the entire garlic genome to elucidate the characteristics of the AsHSF gene family.

Structural Insights into the Catalytic Activity of -Acetylglucosamine Deacetylase.

-Acetylglucosamine deacetylase from (CmCBDA) is a highly effective and selective biocatalyst for the production of d-glucosamine (GlcN) from -acetylglucosamine (GlcNAc). However, the underlying catalytic mechanism remains elusive. Here, we show that CmCBDA is a metalloenzyme with a preference for Ni over Mn.

Benzo[a]pyrene and a high-fat diet induce aortic injury and promote low-density lipoprotein accumulation in the endothelium.

Benzo[a]pyrene (BaP) is a ubiquitous environmental pollutant which mainly exposed though diet. High-fat diet (HFD) can induce atherosclerosis, as can BaP. Unhealthy dietary habits lead to high intake of both BaP and lipids.

The effects of a low carbohydrate diet combined with partial meal replacement on obese individuals.

Objective: We explored the dietary effects of replacing normal dietary staple foods with supplementary nutritional protein powder, dietary fiber, and fish oil on several metabolic parameters. We examined weight loss, glucose and lipid metabolism, and intestinal flora in obese individuals when compared with individuals on a reduced staple food low carbohydrate diet.

Methods: From inclusion and exclusion criteria, 99 participants (28 kg/m ≤ body mass index (BMI) ≤ 35 kg/m) were recruited and randomly assigned to control and intervention 1 and 2 groups.

Parental, Teacher and Peer Effects on the Social Behaviors of Chinese Adolescents: A Structural Equation Modeling Analysis.

Adolescent behavior is closely related to academic and long-term personal development, and adolescents are vulnerable to the influences from people around them. This study aimed to analyze the factors and mechanisms that influence the behavior of adolescents. It examines the impact of family, teachers, and peers on adolescent prosocial behavior and misconduct.

Structural Insights Uncover the Specific Phosphoinositide Recognition by the PH1 Domain of Arap3.

Arap3, a dual GTPase-activating protein (GAP) for the small GTPases Arf6 and RhoA, plays key roles in regulating a wide range of biological processes, including cancer cell invasion and metastasis. It is known that Arap3 is a PI3K effector that can bind directly to PI(3,4,5)P3, and the PI(3,4,5)P3-mediated plasma membrane recruitment is crucial for its function. However, the molecular mechanism of how the protein recognizes PI(3,4,5)P3 remains unclear.

Piranhea trifoliata extracts ameliorate muscular decline in Drosophila melanogaster exposed to Paraquat.

In this study, we have demonstrated, for the first time, the muscular protective effects of Piranhea trifoliata bark extract against Paraquat (PQ)-induced oxidative stress in Drosophila melanogaster. Exposure of D. melanogaster (Canton Special) to PQ caused oxidative stress, as evidenced by protein carbonyl and elevated acetylcholinesterase (AChE) activity levels.

Proteomic Analysis of Human Milk Reveals Nutritional and Immune Benefits in the Colostrum from Mothers with COVID-19.

Despite the well-known benefits of breastfeeding and the World Health Organization's breastfeeding recommendations for COVID-19 infected mothers, whether these mothers should be encouraged to breastfeed is under debate due to concern about the risk of virus transmission and lack of evidence of breastmilk's protective effects against the virus. Here, we provide a molecular basis for the breastfeeding recommendation through mass spectrometry (MS)-based proteomics and glycosylation analysis of immune-related proteins in both colostrum and mature breastmilk collected from COVID-19 patients and healthy donors. The total protein amounts in the COVID-19 colostrum group were significantly higher than in the control group.

Experimental Infection with Sporulated Oocysts of Eimeria maxima (Apicomplexa: Eimeriidae) in Broiler.

Through this study we assessed the metabolic and pathological changes in broilers experimentally infected with oocysts of Eimeria maxima. To perform the experiment, we used 150 broiler strain cooB males, with ten days of age, were randomized according to weight and randomly assigned to two experimental groups: the control group was inoculated with 0.5 mL of distilled water; the infected group inoculated with 0.