Isolation and Characterization of Homologically Expressed Methanol Dehydrogenase from AM1 for the Development of Bioelectrocatalytical Systems.

(Ca)-dependent pyrroloquinolinequinone (PQQ)-dependent methanol dehydrogenase (MDH) (EC: 1.1.2.

Hydrocarbons Biodegradation by : Assimilation of Hexadecane in Different Aggregate States.

The aim of our study was to reveal the peculiarities of the adaptation of rhodococci to hydrophobic hydrocarbon degradation at low temperatures when the substrate was in solid states. The ability of actinobacteria (strains X5 and S67) to degrade hexadecane at 10 °C (solid hydrophobic substrate) and 26 °C (liquid hydrophobic substrate) is described. Despite the solid state of the hydrophobic substrate at 10 °C, bacteria demonstrate a high level of its degradation (30-40%) within 18 days.

Laccase isoform diversity in basidiomycete Lentinus strigosus 1566: Potential for phenylpropanoid polymerization.

Three laccase isoforms with different physicochemical properties could be purified from culture liquid of basidiomycete Lentinus strigosus 1566 obtained during submerged cultivation. The purified laccases possessed individual selectivity in relation to different phenolic compounds. Laccases I, II, and III (59, 65, and 61 kDa respectively) were more active in acidic conditions at around 70 °C.

Developing a technique to enhance durability of fibrous ion-exchange resin substrate for space greenhouses.

One way to cut consumables for space plant growth facilities (PGF) with artificial soil in the form of fibrous ion-exchange resin substrate (FIERS) is on-board regeneration of the used medium. After crop harvest the procedure includes removal of the roots from the fibrous media with preservation of the exchanger properties and capillary structure. One type of FIERS, namely BIONA-V3ۛ, has been used in Russian prototypes of space conveyors.

Adaptation of the white-rot basidiomycete Panus tigrinus for transformation of high concentrations of chlorophenols.

During feed-batch cultivation of the white-rot fungus Panus tigrinus in a 5-l bioreactor on N-limited medium, 100, 200, 500, 1,000 and 2,000 mg 2,4,6-trichlorophenol (2,4,6-TCP) l(-1) were added sequentially after 90% removal of the previous portion of the toxicant. The addition of 500 mg 2,4,6-TCP l(-1) without preliminary adaptation killed the culture. The addition of 300 mg 2,4,6-TCP l(-1) without prior adaptation resulted in its slower removal than removal of 2,000 mg 2,4,6-TCP l(-1) by this adapted culture.

Transformation of 2,4,6-trichlorophenol by free and immobilized fungal laccase.

Laccase from the white rot fungus Coriolus versicolor was immobilized on Celite R-637 by covalent binding with glutaraldehyde. After a sharp primary decline in activity (up to 50%), the retained enzyme activity was stable over a storage period of 33 days at 4 degrees C. A comparative study of soluble and immobilized laccases revealed the increased resistance of immobilized enzyme to the unfavourable effects of alkaline pH, high temperature and the action of inhibitors.

A phage T4 site-specific endonuclease, SegE, is responsible for a non-reciprocal genetic exchange between T-even-related phages.

The bacteriophage T4 segE gene encoding site-specific endonuclease lies between the hoc.1 and uvsW genes. The similar region of T-even-related phage RB30 lacks the segE gene.