Expressions and Clinical Significance of Met and YAP in Gastric Cancer Tissue Microarray.

Objective: This study is aimed at investigating the expression of Met and YAP in gastric cancer and their impact on clinical prognosis.

Methods: Tissue samples and clinical data were collected from 89 patients with gastric cancer. Immunohistochemistry was performed to quantify the expression of Met and YAP using tissue microarray.

Polysaccharide and PD1-Antibody Combination Therapy Enhances Antitumor Efficacy in Colorectal Cancer-Xenograft Mice.

Objective: To observe the efficacy of polysaccharide (AEPS) combined with PD1 antibody therapy in colorectal cancer-xenograft mice.

Methods: CT26 cells were inoculated into 80 C57BL/6 mice to establish the colorectal cancer xenograft-mouse model. Mice were divided evenly into a model group, AEPS group, anti-PD1 group, and combined group.

Physapubescin B enhances the sensitivity of gastric cancer cells to trametinib by inhibiting the STAT3 signaling pathway.

Given the poor prognosis of unresectable advanced gastric cancer (GC), novel therapeutic strategies are needed. The mitogen-activated protein kinase (MAPK) signaling cascade, the most frequently activated pathway in GC, plays an important role in tumorigenesis and metastasis. The MAPK/extracellular signal-regulated kinase (ERK) pathway is an attractive therapeutic target for GC.

The Antipsychotic Agent Sertindole Exhibited Antiproliferative Activities by Inhibiting the STAT3 Signaling Pathway in Human Gastric Cancer Cells.

Gastric cancer (GC) is the third leading cause of cancer-related death. Although the therapeutic approaches have improved, the 5-year survival rate of GC patients after surgical resection remains low due to the high rates of metastasis and recurrence. Patients with schizophrenia have significantly lower incidences of cancer after long-term drug treatment, suggesting the potential or partially ameliorate the risk of cancer development of antipsychotic drugs.

Ursolic Acid Inhibits Epithelial-Mesenchymal Transition through the Axl/NF-B Pathway in Gastric Cancer Cells.

Background: Ursolic acid (UA) is an antitumor component derived from Chinese herbal medicine; this study is to observe the effects of UA on epithelial-mesenchymal transition (EMT) in gastric cancer.

Methods: (1) experiments: 25mol/L and 50mol/L UA were applied to BGC-823, AGS, MGC-803, and HGC-27 cells; MTT staining, Transwell assay, and flow cytometry were used to assess cell proliferation, cell migration, and apoptosis, respectively. Western blot was performed to detect the expressions of N-Cadherin, Vimentin, Snail, Twist, Axl, p-Axl, IKK, p-IKK, NF-B, and p-NF-B.

Differential Amino Acid, Carbohydrate and Lipid Metabolism Perpetuations Involved in a Subtype of Rheumatoid Arthritis with Chinese Medicine Cold Pattern.

Pattern classification is a key approach in Traditional Chinese Medicine (TCM), and it is used to classify the patients for intervention selection accordingly. TCM cold and heat patterns, two main patterns of rheumatoid arthritis (RA) had been explored with systems biology approaches. Different regulations of apoptosis were found to be involved in cold and heat classification in our previous works.

Synergy evaluation by a pathway-pathway interaction network: a new way to predict drug combination.

Drug combinations have been widely applied to treat complex diseases, like cancer, HIV and cardiovascular diseases. One of the most important characteristics for drug combinations is the synergistic effects among different drugs, that is to say, the combination effects are larger than the sum of individual effects. Although quantitative methods can be utilized to evaluate the synergistic effects based on experimental dose-response data, it is both time and resource consuming to screen all possible combinations by experimental trials.

[The history of cupping therapy].

As an important and integral part of non - drug folk therapy on TCM, there were also many other names for cupping therapy, such as "horning", "needlehorn", "sucking method", "fire - cupping", "boiling bamboo cylinder". By textual investigation, considerable clear recognition can be reached on the names, cupping instruments, cupping measure, indications and contradictions in different historical stages. Presently, cupping therapy is combined with other measures, such as drug therapy, acumoxibustions etc.

[Effect of different prescriptions for tonifying kidney on RNA transcription activity in isolated nuclei and protein synthesis activity in free-cell extracts of the different organs of presenile mice].

Objective: To study the anti-aging mechanism of Zuoguiyin (a prescription for tonifying Kindney Yin) and Yishenbao (a prescription for tonifying Kidney Yang) in presenile mice.

Methods: Using male, 14 months old presenile mice as a model of Kidney-Asthenia, the effects of Zuoguiyin and Yishenhao on RNA transcription activity in isolated nuclei and protein synthesis activity in free-cell extracts of the liver, brain, kidney and testes of the presenile mice were observed.

Results: RNA transcription activity in isolated nuclei and protein synthesis activity in free-cell extracts of the four organs of the presenile mice group were distinctly lower than those of young mice group (3 months old).

[Stability examination of beta-cyclodextrin inclusion compound of Zingiber officinale volatile oil].

Based on different storage time and different methods, examination has been conducted of the appearance, properties contents and main constituents in the beta-cyclodextrin inclusion compound of Zingiber officinale volatile oil. The result shows that the oil with inclusion proves considerably more stable than that without inclusion.

[Flow cytometric analysis of the garlic oil effect on DNA content of cancer cell cycle].

Flow cytometry (FCM) is a new technique developed in the recent decade. This technique may measure DNA content of 5000 cells per second and trace the dynamic changes in cell proliferation cycle and offer a hint for designing clinical treatment protocol, monitor prognosis and elucidate the mechanisms of antitumor drugs. The authors previous studies showed significant effect of garlic oil on prolongation of life expectancy and inhibition of tumor growth in mice.

[Effects of ginseng volatile oil on cytochemical components of SGC-823 gastric carcinoma in cell culture].

After 24, 48 and 72 hours' treatment of cells of SGC-823 gastric carcinoma in cell cultures with ginseng volatile oil, glycogen, succinate dehydrogenase and DNA in single cells were measured quantitatively with MPV2 microscope photometer. The results show that the inhibition of cancer cell growth by ginseng volatile oil may be due to the action of metabolism of DNA, carbohydrates and energy.

[Stability examination of the inclusion compounds of 8 Chinese medicinal volatile oils with beta-cyclodextrin].

The content, hence the stability of volatile oils and their main components in the inclusion compounds of 8 Chinese medicinal volatile oils with beta-cyclodextrin have been examined with different storage times. The 8 Chinese medicines are Vitex negundo var. heterophylla, Notopterygium incisum, Alpinia officinarum, Atractylodes lancea, Pogostenon cablin, Asarum heterotropoides var.