[Effect of electroacupuncture on thymocyte apoptosis-related genes in morphine withdrawal rats].

Objective: To explore the underlying mechanism of electroacupuncture (EA) in relieving morphine withdrawal syndrome in rats.

Methods: Forty SD rats were randomly divided into normal control group, model group 1, model group 2 and EA group. Morphine withdrawal syndrome model was established by muscular injection of morphine (5 mg/kg on the 1st day, progressively increasing everyday till 100 mg/kg on the 20th day) in the hind limbs.

[Utilizing modern technology to promote spread and development of acu-moxibustion learning].

Nowadays, modern information technology (IT) has been gained great development. How to use the advanced IT to protect, spread and develop Chinese traditional culture of acupuncture is an important topic at the moment. Based on expounding characteristics of modern IT, the present article analyzed its advantages in digitalizing, exhibiting and sharing the rich resources of acu-moxibustion.

[Correlation between the therapeutic efficacy of electroacupuncture of ear-body acupoints and mast cells in experimental gastric ulcer rats].

Objective: To study the interrelation between the therapeutic efficacy of electroacupuncture (EA) and mast cells (MC) in experimental gastric ulcer rats.

Methods: Thirty Wistar rats were randomly divided into normal control group, model group and EA group, with 10 cases in each. Experimental gastric ulcer model was established by injecting 20% glacial acetic acid (0.

[Basic factors for inducing divarication about the location of some acupoints].

The curative effect of acupuncture depends much on accurate location of acupoints. But, in the developmental course of Chinese acu-moxibustion medicine, some divarications about the location of acupoints have occurred for ages. In the process of formulating international standard of acupoint location, experts from China, Japan and Korea had different viewpoints about the location of 92 acupoints.

[Effects of strong and weak electroacupuncture on endotoxin-induced changes of electrical activities of heat-sensitive neurons in preoptic area and anterior hypothalamus in rabbits].

Objective: To observe the effects of weak and strong electroacupuncture (EA) on endotoxin (ET) thermolysis-induced changes of discharges of neurons in the preoptic region and anterior hypothalamus (PO-AH) so as to explore its underlying mechanism in antipyretic and thermolytic actions and its relation to the receptive system of acupoints.

Methods: Eighteen New Zealand rabbits were randomly divided into ET group, ET + weak-EA group and ET + strong-EA group. Extracellular discharges of the PO-AH neurons were recorded by using tungsten microelectrodes.

[Effects of electroacupuncture of different acupoints on cardiac function in acute myocardial ischemia rabbits].

Objective: To observe the effects of electroacupuncture (EA) of "Neiguan" (PC 6), "Shenmen" (HT 7), "Taiyuan" (LU 9), "Zhizheng" (SI 7) and "Sanyinjiao" (SP 6) on cardiac function in acute myocardial ischemia (AMI) rabbits.

Methods: A total of 60 rabbits were randomly divided into normal control, model, EA-PC6, EA-HT7, EA-LU9, EA-S17 and EA-SP6 groups. AMI model was established by intravenous administration of pituitrin.

[Observation on the accumulative analgesic effect of electroacupuncture and the expression of protein kinase A in hypothalamus and hippocampus in chronic pain or/and ovariectomized rats].

Objective: To observe the accumulative analgesic effect of electroacupuncture (EA) and the simultaneous changes of protein kinase A (PKA) expression in hypothalamus and hippocampus in rats with chronic constrictive injury (CCI) or/and learning-memory impairment.

Methods: Sixty-eight female Wistar rats were randomized into normal control, CCI, CCI + EA 2 d, CCI + EA 2 w, OVX + CCI, OVX + CCI + EA 2 d and OVX + CCI + EA 2 w groups, with 10 cases in each except OVX + CCI group (n=8). CCI pain model was established by ligature of the right sciatic nerve with surgical suture.

[Effects of electroacupuncture pretreatment on ischemic arrhythmia and intracellular calcium oscillation in single cardiocyte isolated from rats with myocardial ischemia and reperfusion].

Objective: To observe the inhibitory effects of acupuncture pretreatment on arrhythmia and intracellular calcium ([Ca2+]i) oscillation in single cardiocyte in rats subjected to myocardial ischemia-reperfusion (MIR).

Methods: Male SD rats were evenly randomized into normal control (NC), MIR and electroacupuncture (EA) groups. MIR was performed by ligation (30 min) and reperfusion (10 min) of the left anterior descending coronary artery in vivo in rats or in the isolated and perfused rat heart subjected to a 40 min global ischemia followed by a 10 min reperfusion.

[Effect of electroacupuncture and moxibustion of "Dazhui" (GV14) on the number and distribution of degranulated mast cells in GV14 region].

Objective: To compare the effects of electroacupuncture (EA) and moxibustion on the degranulated mast cell number and distribution in "Dazhui" (GV14) region of rats.

Methods: Fifteen Wistar rats were randomly divided into normal control, EA and moxibustion groups, with 5 cases in each group. "Dazhui" (GV14) was stimulated by EA (3/10 Hz, 2-4 V, 20 mm) or moxibustion (2 cones, 20 mm), thereafter, the tissues of GV14 region were sampled, quickly frozen, sectioned (20 microm) and stained with toluidine blue separately for observing the degranulated state of mast cells under microscope.

[Effect of acupuncture of different acupoints on electrical activities of hypothalamic sexual arousal stimulation-related neurons at different stages of oestrous cycle in rats].

Objective: To compare the effects of acupuncture stimulation of different acupoints on discharges of hypothalamic sexual arousal stimulation-related excitatory neurons in the rat so as to selected effective acupoints for regulating genito-endocrine function.

Methods: Experiments were performed in 32 female Sprague-Dawley rats. Examination of vaginal castoff cell smear (HE staining) was performed to determine the rats' estrous cycle.

[Observation on the analgesic effect of repeated electroacupuncture and its relation to changes of plasma beta-EP, ACTH and COR levels].

Objective: To observe the regularity of electroacupuncture (EA) analgesia and to analyze its relation to hypothalamus-pituitary-adrenal cortex axis function in chronic constrictive injury (CCI) and ovariectomy (OVX) rats.

Methods: A total of 110 Wistar rats anesthetized with mixture solution of 1.5% chloralose (50 mg/kg) and 25% urethrane (420 mg/kg, i.

[Combined application of surface anatomy, imageology and anthropometry to the study on standardization of acupoint location].

In the process of revising national standard of acupoint location and formulating international standard of acupoint location, the author of the present paper, using surface anatomy (a new branch of anatomy) and imageological methods, successfully solves some problems with which are difficult to dealt merely according to literature analysis, not only providing scientific evidence for formulating both national and international standards of acupoint location, but also uncovering scientific value of experience of surface anatomy accumulated systematically in Chinese ancient times and revealing its new vitality. It provides an extensive applied prospect for the development of surface anatomy.

[Experimental verification of "treating lumbar-back problems by puncturing Weizhong (BL 40)" with blood perfusion imaging technique].

Objective: To verify the classic saying of "Treating lumbar-back problems by puncturing Weizhong (BL 40)" in acu-moxibustion learning and to explore its underlying mechanism.

Methods: Forty volunteer sublects were asked to accept twice tests respectively, i.e.

[Effects of electroacupuncture of "Shenmen" (HT 7) and "Zhizheng" (SI 7) on cardiac function and electrical activities of cardiac sympathetic nerve in acute myocardial ischemia rabbits].

Objective: To observe the effect of electroacupuncture (EA) of "Shenmen" (HT 7) and "Zhizheng" (SI 7) on cardiac function and electrical activities of the cardiac sympathetic nerve in acute myocardial schemia rabbits.

Methods: Forty livid-blue rabbits were randomly divided into normal control, model, "Shenmen" (HT 7) and "Zhizheng" (517) groups with 10 cases in each group. Acute myocardial ischemia (AMI) model was established by intravenous injection of pituitrin (3 U/kg).

[Effect of electroacupuncture on the contents of excitatory amino acids in cerebral tissue at different time courses in rats with cerebral ischemia and reperfusion injury].

Objective: To observe changes of contents of excitatory amino acids in focal cerebral ischemic tissue after electroacupuncture (EA) treatment at different time courses in cerebral ischemia and reperfusion (CI/R) rats so as to provide experimental evidence for selecting the best duration of EA stimulation in the treatment of apoplexy.

Methods: Forty SD rats were randomized into control, model, EA-10 min, EA-30 min and EA-60 min groups. CI/R model was established by middle cerebral artery occlusion (MCAO) and reperfusion.

[Progress in the study on the relationship between effects of acu-moxibustion and mast cells in acupoints].

The present paper reviews develonment of researches on the relationship between mast cells (MCs) and effects of acupuncture and moxibustion from (1) the formation, distribution, types, morphological characters of MCs and their biological effects, (2) the number and degranulation of MCs in acupoints, (3) the relavance of MCs and the phenomenon of sensation transmission along meridian, and 4) the correlation between the therapeutic effect of acupuncture for relieving allergic reaction and MCs. Researches show that (1) MCs act as one of the characteristic constituents in acupoints where the MCs are in close relation with the effects of acupuncture and moxibustion, (2) MCs participate in the regulatory process of the organism in physiology and pathology. The underlying mechanism of MCs-related neuro-humoral regulation and meridian needs to be studied further.

[Summary of the research on Master Dou's Canon of Acupuncture].

Doutaishi Zhenjing ("[Chinese characters: see text]" Master DOU's Canon of Acupuncture) is a monograph on acupoints, without spreading widely. Now, there exist two manuscript writings, namely, Yang's Jiachuan Zhenjing Tuxiang ("[Chinese characters: see text]") YANG's Patrimonial Acupuncture Canon and Pictures) and Yulong Ge ("[Chinese characters: see text]") Jade Dragon Verse), but both are not complete. The maneuvers of acupuncture needle and indications of acupoints recorded in Master DOU's Canon of Acupuncture still have an important clinical significance in guiding current acupuncture practice.

[Effect of acupuncture of "Zusanli" (ST 36) on serum gastrin and cortisol contents in rats with spleen deficiency syndrome].

Objective: To study the underlying mechanism of acupuncture of "Zusanli" (ST 36) in the treatment of spleen deficiency syndrome.

Methods: Twenty-six adult Wistar rats were randomly divided into normal control group (n = 6), model group (n = 8), saline group (n = 6) and acupuncture group (n = 6). Spleen deficiency syndrome model was duplicated by intragastric perfusion of 50% alcohol (on the 1st day, 1 mL/100 g) and white vinegar for 9 days (1 mL/100 g).

[Regulative effect of electroacupuncture on hypothalamus-hypophysis-gonad axis in different stages of estrous cycles in rats].

Objective: To investigate the specific acupoint, optimal curative opportunity and the involved pathway of electroacupuncture (EA) for regulating gono-endocrine hormones.

Methods: (1) Adult female rats were randomly divided into sham-EA group (n = 32), "Guanyuan" (CV 4) group (n = 36) and "Neiguan" (PC 6) group (n = 36) which were further divided into diestrum, preoestrus (P-EST), estruation and metaoestrus subgroups according to tetra-estrous cycles determined by vaginal smear test. EA (20/60 Hz, 2-3 mA) was applied to CV4 and PC6 separately for 30 min from about 9:30 to 10:00 Am.

[Effect of acupuncture on learning-memory ability in diabetic rats with concomitant cerebral ischemia-reperfusion injury].

Objective: To observe the effect of acupuncture on learning and memory ability in rats with cognition impairment due to diabetes mellitus (DM) and cerebral ischemia (CI)/reperfusion injury (CI/RI).

Methods: A total of 87 female Wistar rats were randomly divided into 5 groups: normal control, CI, DM+ sham-CI, DM+CI and DM+ CI+ acupuncture (Acup). DM model was induced by intraperitoneal injection of streptozotocin (60 mg/kg), and 3 days later CL/RI was duplicated by occlusion of the bilateral carotid arteries and reperfusion in the same rats.