Streamlining Global Germplasm Exchange: Integrating Scientific Rigor and Common Sense to Exclude Phantom Agents from Regulation.

This collaborative work by over 180 researchers from 40+ countries addresses the challenges posed by "phantom agents"-putative pathogenic agents named in literature without supporting data on their existence. Those agents remain on regulatory lists, creating barriers in trade and plant certification. Historically identified based solely on symptoms, these agents lack isolates or sequence data, making reliable detection or risk assessment impossible.

Onion ( L.) as a new host for ' Arsenophonus phytopathogenicus' in Germany.

Onion ( L.) is the most produced vegetable after tomato worldwide and is grown on about 15,000 ha in Germany. In Lampertheim, Hesse in southwest Germany (49°40'02.

The complete genome of " Phytoplasma fraxini" AshY1 from the ash yellows group.

The complete genome of " Phytoplasma fraxini" AshY1, originating from in North America, was assembled using long reads from single-molecule real-time sequencing technology. The chromosome of 598 kb provides insights into the effector repertoire of a phytopathogenic bacterium from the 16SrVII phytoplasma group.

Evaluation of a push-and-pull strategy using volatiles of host and non-host plants for the management of pear psyllids in organic farming.

Introduction: Pear decline (PD) is one of the most devastating diseases of in Europe and North America. It is caused by the pathogen Phytoplasma pyri' and transmitted by pear psyllids (, , and ). Identifying attractant and repellent volatile organic compounds (VOCs) could improve the development of alternative plant protection measurements like push-pull or attract-and-kill strategies against pear psyllids.

Bridging biotremology and chemical ecology: a new terminology.

Living organisms use both chemical and mechanical stimuli to survive in their environment. Substrate-borne vibrations play a significant role in mediating behaviors in animals and inducing physiological responses in plants, leading to the emergence of the discipline of biotremology. Biotremology is experiencing rapid growth both in fundamental research and in applications like pest control, drawing attention from diverse audiences.

Arthropods as Vectors of Grapevine Trunk Disease Pathogens: Quantification of on Arthropods and Mycobiome Analysis of Earwig Exoskeletons.

Viticulture worldwide is challenged by grapevine trunk diseases (GTDs). Involvement of arthropods in the dissemination process of GTD pathogens, notably esca pathogens, is indicated after detection of associated pathogens on arthropod exoskeletons, and demonstration of transmission under artificial conditions. The present study is the first to quantify spore loads via qPCR of the esca-relevant pathogen on arthropods collected in German vineyards, i.

Adhesion Peptide-Functionalized Biobased Microgels for Controlled Delivery of Pesticides.

Widespread use of plant protection agents in agriculture is a major cause of pollution. Apart from active ingredients, the environmental impact of auxiliary synthetic polymers should be minimized if they are highly persistent. An alternative to synthetic polymers is the use of natural polysaccharides, which are abundant and biodegradable.

(Linnaeus, 1761) as a Vector of Phloem-Restricted Pathogens on Potatoes: ' Arsenophonus Phytopathogenicus' and ' Phytoplasma Solani'.

In Germany, the phloem-sucking planthopper (Hemiptera: Cixiidae) currently represents the epidemiological driver for the spread of the syndrome "Basses Richesses" in sugar beets, which results in a reduced sugar content and an economic loss for the farmers. This disease is associated with the γ-proteobacterium ' Arsenophonus phytopathogenicus' and the Stolbur phytoplasma ' Phytoplasma solani'. Recently, was found in potato fields in Germany and is associated with Stolbur-like symptoms in this crop.

Polysaccharide Peptide Treatment Eliminates Strawberry Viruses and Promotes Strawberry Plant Growth and Rooting in Tissue Culture Media.

Based on our previous finding that polysaccharide peptide (PSP) has substantial antiviral activity, we cultured strawberry plants infected with strawberry mild yellow edge virus (SMYEV) or strawberry vein banding virus (SVBV) in Murashige and Skoog (MS) media supplemented with PSP to test its ability to eliminate these viruses. PSP not only improved the elimination of SMYEV and SVBV but also promoted the growth and rooting of strawberry plants in tissue culture. On the 45th day, the average height of the 'Ningyu' strawberry plants in the 1-mg/ml PSP treatment group was 1.

Toxicity of Pesticides Applied in European Vineyards on and , Parasitoids of and .

Risk assessments of chemical pesticides toward natural enemies are crucial for ensuring sustainable grapevine-integrated pest management. In this context, laboratory experiments were conducted to evaluate the toxicity of four insecticides (lambda-cyhalothrin, flupyradifurone, acetamiprid, and cyantraniliprole) and one fungicide (spiroxamine) commonly applied in German (European) vineyards on the pupae and adults of both , a parasitoid of the vine mealybug , and , a parasitoid of the European grapevine moth, . The tested pesticides did not significantly affect the development of the pupal stage inside mealybug mummies or the emergence of the parasitoid .

Risk Assessment for the Spread of Flavescence Dorée-Related Phytoplasmas from Alder to Grapevine by Alternative Insect Vectors in Germany.

"Flavescence dorée" (FD)-related phytoplasmas are widespread in alder in Germany and their transmission to grapevine represents a high risk for FD outbreaks when the primary vector, , becomes present in the future. Therefore, the potential role of the Deltocephalinae leafhopper species in transmitting FD-related phytoplasmas from alder to grapevine was studied in extensive transmission trials conducted between 2017 and 2020. The transmission capacity of autochthonous spp.

Pandora cacopsyllae Eilenberg, Keller & Humber (Entomophthorales: Entomophthoraceae), a new species infecting pear psyllid Cacopsylla pyri L. (Hemiptera: Psyllidae).

The new species Pandora cacopsyllae Eilenberg, Keller & Humber (Entomophthorales) is described. The fungus was found on infected pear psyllids Cacopsylla pyri (Hemiptera: Psyllidae) in a pear orchard in Zealand, Denmark. Morphological structures (conidia, rhizoids, cystidia) were described on the designated type host C.

Complete Genome of " Phytoplasma rubi" RS, a Phytopathogenic Bacterium Associated with Stunt Disease.

The phytoplasma " Phytoplasma rubi" is associated with stunt disease. The complete genome was determined by assembling Oxford Nanopore Technologies system-derived long reads, with short-read polishing with Illumina reads. The genome of strain RS, from Germany, is organized in one circular chromosome with a length of 762 kb.

Arthropods on grapes benefit more from fungicide reduction than from organic farming.

Background: Pesticides are considered main contributors to global arthropod declines and therefore may decrease the provision of ecosystem services such as natural pest control. Organic farming and cultivating pest- and disease-resistant varieties can allow pesticide applications and their impacts on nontarget organisms and the environment to be reduced. We investigated the effects of organic versus conventional management and fungus-resistant versus susceptible wine grape varieties on arthropod biodiversity and pest control of grape berry moths in 32 vineyards in the Palatinate region, Germany.

Altered volatile emission of pear trees under elevated atmospheric CO levels has no relevance to pear psyllid host choice.

The impact of climate change drivers on cultivated plants and pest insects has come into research focus. One of the most significant drivers is atmospheric carbon dioxide, which is converted into primary plant metabolites by photosynthesis. Increased atmospheric CO concentrations therefore affect plant chemistry.

Repellent Activity of Clove Essential Oil Volatiles and Development of Nanofiber-Based Dispensers against Pear Psyllids (Hemiptera: Psyllidae).

Pear psyllids are the main vectors of the pathogen ' Phytoplasma pyri' causing pear decline. Based on earlier reports, we tested the behavioral activity of the major synthetic compounds of clove essential oil (eugenol, eugenyl acetate, and β-caryophyllene) against and . Of six mixtures tested in olfactometer assays, a formulation consisting of three specific compounds (M6 mixture) demonstrated a repellent effect on both psyllid species.

Using Malaise Traps and Metabarcoding for Biodiversity Assessment in Vineyards: Effects of Weather and Trapping Effort.

Metabarcoding is a powerful tool for ecological studies and monitoring that might provide a solution to the time-consuming taxonomic identification of the vast diversity of insects. Here, we assess how ambient weather conditions during Malaise trap exposure and the effort of trapping affect biomass and taxa richness in vineyards. Biomass varied by more than twofold with weather conditions.

Revision of the ' Phytoplasma' species description guidelines.

The genus ' Phytoplasma' was proposed to accommodate cell wall-less bacteria that are molecularly and biochemically incompletely characterized, and colonize plant phloem and insect vector tissues. This provisional classification is highly relevant due to its application in epidemiological and ecological studies, mainly aimed at keeping the severe phytoplasma plant diseases under control worldwide. Given the increasing discovery of molecular diversity within the genus '.

Ecology and Evolutionary History of Beetles-Overview and Update.

An overview is given on several aspects of evolutionary history, ecology, host plant use, and pharmacophagy of spp. with a focus on the evolution of host plant breadth and effects of plant compounds on natural enemies used for biocontrol of pest species in the group. Recent studies on each aspect are discussed, latest publications on taxonomic grouping of spp.

Encapsulation of the psyllid-pathogenic fungus Pandora sp. nov. inedit. and experimental infection of target insects.

Background: Pandora sp. nov. inedit.

Climate change risk to pheromone application in pest management.

Since of the first sex pheromone and the adoption of pheromone in pest management, the global pheromone market size has grown to reach USD 2.4 billion per year in 2019. This has enabled the development of environmentally friendly approaches that significantly reduce the application of pesticides.

Feeding Volatiles of Larval Sparganothis pilleriana (Lepidoptera: Tortricidae) Attract Heterospecific Adults of the European Grapevine Moth.

Plants release volatiles in response to caterpillar feeding. These herbivore-induced plant volatiles (HIPVs) attract natural enemies of the herbivores and repel or attract conspecific adult herbivores in a tri-trophic interaction which has been considered to be an indirect plant defense against herbivores. Recently, we demonstrated the attraction of male and female European grapevine moth, Lobesia botrana (Denis & Schiffermüller) (Lepidoptera: Tortricidae) to a blend of phenylacetonitrile and acetic acid, two compounds identified as HIPVs in heterospecific apple-leafroller interactions.