Comparative Genomic Analysis of the Regulation of Aromatic Metabolism in Betaproteobacteria.

Aromatic compounds are a common carbon and energy source for many microorganisms, some of which can even degrade toxic chloroaromatic xenobiotics. This comparative study of aromatic metabolism in 32 Betaproteobacteria species describes the links between several transcription factors (TFs) that control benzoate (BenR, BenM, BoxR, BzdR), catechol (CatR, CatM, BenM), chlorocatechol (ClcR), methylcatechol (MmlR), 2,4-dichlorophenoxyacetate (TfdR, TfdS), phenol (AphS, AphR, AphT), biphenyl (BphS), and toluene (TbuT) metabolism. We characterize the complexity and variability in the organization of aromatic metabolism operons and the structure of regulatory networks that may differ even between closely related species.

UAV Landing Based on the Optical Flow Videonavigation.

An automatic landing of an unmanned aerial vehicle (UAV) is a non-trivial task requiringa solution of a variety of technical and computational problems. The most important is the precisedetermination of altitude, especially at the final stage of approaching to the earth. With currentaltimeters, the magnitude of measurement errors at the final phase of the descent may be unacceptablyhigh for constructing an algorithm for controlling the landing manoeuvre.

ISLES 2016 and 2017-Benchmarking Ischemic Stroke Lesion Outcome Prediction Based on Multispectral MRI.

Performance of models highly depend not only on the used algorithm but also the data set it was applied to. This makes the comparison of newly developed tools to previously published approaches difficult. Either researchers need to implement others' algorithms first, to establish an adequate benchmark on their data, or a direct comparison of new and old techniques is infeasible.

New Approaches to the Integration of Navigation Systems for Autonomous Unmanned Vehicles (UAV).

The article presents an overview of the theoretical and experimental work related to unmanned aerial vehicles (UAVs) motion parameters estimation based on the integration of video measurements obtained by the on-board optoelectronic camera and data from the UAV's own inertial navigation system (INS). The use of various approaches described in the literature which show good characteristics in computer simulations or in fairly simple conditions close to laboratory ones demonstrates the sufficient complexity of the problems associated with adaption of camera parameters to the changing conditions of a real flight. In our experiments, we used computer simulation methods applying them to the real images and processing methods of videos obtained during real flights.

Microbiomes of gall-inducing copepod crustaceans from the corals Stylophora pistillata (Scleractinia) and Gorgonia ventalina (Alcyonacea).

Corals harbor complex and diverse microbial communities that strongly impact host fitness and resistance to diseases, but these microbes themselves can be influenced by stresses, like those caused by the presence of macroscopic symbionts. In addition to directly influencing the host, symbionts may transmit pathogenic microbial communities. We analyzed two coral gall-forming copepod systems by using 16S rRNA gene metagenomic sequencing: (1) the sea fan Gorgonia ventalina with copepods of the genus Sphaerippe from the Caribbean and (2) the scleractinian coral Stylophora pistillata with copepods of the genus Spaniomolgus from the Saudi Arabian part of the Red Sea.

[Progress of sleep studies in the age of electrophysiology. The visceral theory of sleep].

Electrophysiological methods of studying the nervous system have opened up new opportunities for investigations of sleep. Striking changes in the pattern of EEG during the transition from wakefulness to sleep made it's recording a mandatory element of any somnological research. It was also found that the frequency of neuronal firing in the cerebral cortex during sleep does not decrease, but can significantly exceed the average level of the cortical activity during wakefulness.

Nucleotide patterns aiding in prediction of eukaryotic promoters.

Computational analysis of promoters is hindered by the complexity of their architecture. In less studied genomes with complex organization, false positive promoter predictions are common. Accurate identification of transcription start sites and core promoter regions remains an unsolved problem.

Identification of Transcriptional Regulators of Psoriasis from RNA-Seq Experiments.

Psoriasis is a common inflammatory skin disease with complex etiology and chronic progression. To provide novel insights into the molecular mechanisms of regulation of the disease we performed RNA sequencing (RNA-Seq) analysis of 14 pairs of skin samples collected from psoriatic patients. Subsequent pathway analysis and an extraction of transcriptional regulators governing psoriasis-associated pathways was executed using a combination of MetaCore Interactome enrichment tool and cisExpress algorithm, and followed by comparison to a set of previously described psoriasis response elements.

Analysis of cis-Regulatory Elements in Gene Co-expression Networks in Cancer.

Analysis of gene co-expression networks is a powerful "data-driven" tool, invaluable for understanding cancer biology and mechanisms of tumor development. Yet, despite of completion of thousands of studies on cancer gene expression, there were few attempts to normalize and integrate co-expression data from scattered sources in a concise "meta-analysis" framework. Here we describe an integrated approach to cancer expression meta-analysis, which combines generation of "data-driven" co-expression networks with detailed statistical detection of promoter sequence motifs within the co-expression clusters.

Coding of spatial attention priorities and object features in the macaque lateral intraparietal cortex.

Primate posterior parietal cortex (PPC) is known to be involved in controlling spatial attention. Neurons in one part of the PPC, the lateral intraparietal area (LIP), show enhanced responses to objects at attended locations. Although many are selective for object features, such as the orientation of a visual stimulus, it is not clear how LIP circuits integrate feature-selective information when providing attentional feedback about behaviorally relevant locations to the visual cortex.

Determination of the strong coupling constant in next-to-next-to-leading order QCD using H1 jet cross section measurements: H1 Collaboration.

The strong coupling constant is determined from inclusive jet and dijet cross sections in neutral-current deep-inelastic scattering (DIS) measured at HERA by the H1 collaboration using next-to-next-to-leading order (NNLO) QCD predictions. The dependence of the NNLO predictions and of the resulting value of at the -boson mass are studied as a function of the choice of the renormalisation and factorisation scales. Using inclusive jet and dijet data together, the strong coupling constant is determined to be .

The Genetic Architecture of Major Depressive Disorder in Han Chinese Women.

Importance: Despite the moderate, well-demonstrated heritability of major depressive disorder (MDD), there has been limited success in identifying replicable genetic risk loci, suggesting a complex genetic architecture. Research is needed to quantify the relative contribution of classes of genetic variation across the genome to inform future genetic studies of MDD.

Objectives: To apply aggregate genetic risk methods to clarify the genetic architecture of MDD by estimating and partitioning heritability by chromosome, minor allele frequency, and functional annotations and to test for enrichment of rare deleterious variants.

Integrated computational approach to the analysis of RNA-seq data reveals new transcriptional regulators of psoriasis.

Psoriasis is a common inflammatory skin disease with complex etiology and chronic progression. To provide novel insights into the regulatory molecular mechanisms of the disease, we performed RNA sequencing analysis of 14 pairs of skin samples collected from patients with psoriasis. Subsequent pathway analysis and extraction of the transcriptional regulators governing psoriasis-associated pathways was executed using a combination of the MetaCore Interactome enrichment tool and the cisExpress algorithm, followed by comparison to a set of previously described psoriasis response elements.

Published GMO studies find no evidence of harm when corrected for multiple comparisons.

A number of widely debated research articles claiming possible technology-related health concerns have influenced the public opinion on genetically modified food safety. We performed a statistical reanalysis and review of experimental data presented in some of these studies and found that quite often in contradiction with the authors' conclusions the data actually provides weak evidence of harm that cannot be differentiated from chance. In our opinion the problem of statistically unaccounted multiple comparisons has led to some of the most cited anti-genetically modified organism health claims in history.

UAV Control on the Basis of 3D Landmark Bearing-Only Observations.

The article presents an approach to the control of a UAV on the basis of 3D landmark observations. The novelty of the work is the usage of the 3D RANSAC algorithm developed on the basis of the landmarks' position prediction with the aid of a modified Kalman-type filter. Modification of the filter based on the pseudo-measurements approach permits obtaining unbiased UAV position estimation with quadratic error characteristics.

GntR Family of Bacterial Transcription Factors and Their DNA Binding Motifs: Structure, Positioning and Co-Evolution.

The GNTR family of transcription factors (TFs) is a large group of proteins present in diverse bacteria and regulating various biological processes. Here we use the comparative genomics approach to reconstruct regulons and identify binding motifs of regulators from three subfamilies of the GNTR family, FADR, HUTC, and YTRA. Using these data, we attempt to predict DNA-protein contacts by analyzing correlations between binding motifs in DNA and amino acid sequences of TFs.

Hidden Markov models for evolution and comparative genomics analysis.

The problem of reconstruction of ancestral states given a phylogeny and data from extant species arises in a wide range of biological studies. The continuous-time Markov model for the discrete states evolution is generally used for the reconstruction of ancestral states. We modify this model to account for a case when the states of the extant species are uncertain.

A manually curated database of tetrapod mitochondrially encoded tRNA sequences and secondary structures.

Background: Mitochondrial tRNAs have been the subject of study for structural biologists interested in their secondary structure characteristics, evolutionary biologists have researched patterns of compensatory and structural evolution and medical studies have been directed towards understanding the basis of human disease. However, an up to date, manually curated database of mitochondrially encoded tRNAs from higher animals is currently not available.

Description: We obtained the complete mitochondrial sequence for 277 tetrapod species from GenBank and re-annotated all of the tRNAs based on a multiple alignment of each tRNA gene and secondary structure prediction made independently for each tRNA.

Modeling classic attenuation regulation of gene expression in bacteria.

A model is proposed primarily for the classical RNA attenuation regulation of gene expression through premature transcription termination. The model is based on the concept of the RNA secondary structure macrostate within the regulatory region between the ribosome and RNA-polymerase, on hypothetical equation describing deceleration of RNA-polymerase by a macrostate and on views of transcription and translation initiation and elongation, under different values of the four basic model parameters which were varied. A special effort was made to select adequate model parameters.

Purifying selection in mitochondria, free-living and obligate intracellular proteobacteria.

Background: The effectiveness of elimination of slightly deleterious mutations depends mainly on drift and recombination frequency. Here we analyze the influence of these two factors on the strength of the purifying selection in mitochondrial and proteobacterial orthologous genes taking into account the differences in the organism lifestyles.

Results: (I) We found that the probability of fixation of nonsynonymous substitutions (Kn/Ks) in mitochondria is significantly lower compared to obligate intracellular bacteria and even marginally significantly lower compared to free-living bacteria.

Computational identification of BioR, a transcriptional regulator of biotin metabolism in Alphaproteobacteria, and of its binding signal.

Comparative genomic analysis was applied to identify the biotin transcriptional regulator, BioR, in most Alphaproteobacteria, and to identify its recognition signal TTATMKATAA. BioR belongs to the GntR family of transcriptional repressors. The functional assignment is supported by three lines of evidence: (1) bioR is positionally clustered with various bio genes, both for biotin biosynthesis and transport; (2) in most cases, candidate BioR-binding sites (BIOR boxes) are observed upstream of the bioR genes, suggesting autoregulation; (3) the phyletic distribution of the BIOR boxes coincides exactly with the phyletic distribution of the bioR genes, as the genomes lacking BIOR boxes do not have orthologs of bioR.

An algorithm for identification of regulatory signals in unaligned DNA sequences, its testing and parallel implementation.

We describe an algorithm (IRSA) for identification of common regulatory signals in samples of unaligned DNA sequences. The algorithm was tested on randomly generated sequences of fixed length with implanted signal of length 15 with 4 mutations, and on natural upstream regions of bacterial genes regulated by PurR, ArgR and CRP. Then it was applied to upstream regions of orthologous genes from Escherichia coli and related genomes.

Methods of horizontal gene transfer determination using phylogenetic data.

A new approach for comparative analysis of multiple trees reconstructed for representative protein families is proposed. This approach is based on the hypothesis of gene duplication, gene loss and horizontal gene transfer and makes use of stochastic methods and optimization. We present a species tree of 40 prokaryotic organisms obtained by our algorithm on the basis of 132 clusters of orthologous groups of proteins (COGs) from the GenBank of the National Center for Biotechnology Information (USA).

[Intracellular localization of calcium in loach embryonic cells at the early gastrula stage].

Intracellular localization of calcium-accumulated structures in the loach embryo cells at the state of early gastrulation was determined by electron microscope histochemical pyroantimonate method. Calcium-precipitate was observed in the nucleus (external and inner membranes, chromatin and nucleolus), and in the cytoplasm (ER, mitochondria and submembrane cortical layer). Our present data show that such cell organelles as nuclear envelope, ER, and mitochondria serve as the basic cellular stores for calcium, confirming the existence of calcium signal system both in the nucleus and in the cytosol.