Interplay between hormones and assimilates during pear development and ripening and its relationship with the fruit postharvest behaviour.

The ability of European pears (Pyrus communis L.) to ripen immediately after harvest is cultivar-dependent and relies on a range of physiological and biochemical events occurring during fruit growth and development that remain largely unknown. To gain further knowledge on these events, changes in the content of sugars, acids, major hormones and ethylene precursors or related enzymes were studied in two pear varieties ('Blanquilla' and 'Conference') with known differences in their postharvest ripening behaviour.

Diat.barcode, an open-access curated barcode library for diatoms.

Diatoms (Bacillariophyta) are ubiquitous microalgae which produce a siliceous exoskeleton and which make a major contribution to the productivity of oceans and freshwaters. They display a huge diversity, which makes them excellent ecological indicators of aquatic ecosystems. Usually, diatoms are identified using characteristics of their exoskeleton morphology.

Discovering consensus genomic regions in wheat for root-related traits by QTL meta-analysis.

Root system architecture is crucial for wheat adaptation to drought stress, but phenotyping for root traits in breeding programmes is difficult and time-consuming owing to the belowground characteristics of the system. Identifying quantitative trait loci (QTLs) and linked molecular markers and using marker-assisted selection is an efficient way to increase selection efficiency and boost genetic gains in breeding programmes. Hundreds of QTLs have been identified for different root traits in the last few years.

Discovery of a kleptoplastic 'dinotom' dinoflagellate and the unique nuclear dynamics of converting kleptoplastids to permanent plastids.

A monophyletic group of dinoflagellates, called 'dinotoms', are known to possess evolutionarily intermediate plastids derived from diatoms. The diatoms maintain their nuclei, mitochondria, and the endoplasmic reticulum in addition with their plastids, while it has been observed that the host dinoflagellates retain the diatoms permanently by controlling diatom karyokinesis. Previously, we showed that dinotoms have repeatedly replaced their diatoms.

From landraces to improved cultivars: Assessment of genetic diversity and population structure of Mediterranean wheat using SNP markers.

Assessment of genetic diversity and population structure in crops is essential for breeding and germplasm conservation. A collection of 354 bread wheat genotypes, including Mediterranean landraces and modern cultivars representative of the ones most widely grown in the Mediterranean Basin, were characterized with 11196 single nucleotide polymorphism (SNP) markers. Total genetic diversity (HT) and polymorphic information content (PIC) were 0.

The Four FAD-Dependent Histone Demethylases of Arabidopsis Are Differently Involved in the Control of Flowering Time.

In , four FAD-dependent lysine-specific histone demethylases (LDL1, LDL2, LDL3, and FLD) are present, bearing both a SWIRM and an amine oxidase domain. In this study, a comparative analysis of gene structure, evolutionary relationships, tissue- and organ-specific expression patterns, physiological roles and target genes for the four Arabidopsis is reported. Phylogenetic analysis evidences a different evolutionary history for the four , while promoter activity data show that are strongly expressed during plant development and embryogenesis, with some gene-specific expression patterns.

Inter-comparison of thermal measurements using ground-based sensors, UAV thermal cameras, and eddy covariance radiometers.

With the increasing availability of thermal proximity sensors, UAV-borne cameras, and eddy covariance radiometers there may be an assumption that information produced by these sensors is interchangeable or compatible. This assumption is often held for estimation of agricultural parameters such as canopy and soil temperature, energy balance components, and evapotranspiration. Nevertheless, environmental conditions, calibration, and ground settings may affect the relationship between measurements from each of these thermal sensors.

Transgenic solutions to increase yield and stability in wheat: shining hope or flash in the pan?

This article comments on: 2019. Field-grown transgenic wheat expressing the sunflower gene significantly outyields the wild type. Journal of Experimental Botany 70, 1669–1681.

Pasta-Making Quality QTLome From Mediterranean Durum Wheat Landraces.

In order to identify genome regions related to pasta-making quality traits, association mapping (AM) was performed in a set of 165 durum wheat landraces from 21 Mediterranean countries. The collection was genotyped using 1149 DArT markers and 872 of them with a known genetic position were used for AM. The collection was grown in north-east Spain during 3 years.

A comprehensive study on the main physiological and biochemical changes occurring during growth and on-tree ripening of two apple varieties with different postharvest behaviour.

Apple quality and the storage potential likely depend on a range of physiological and biochemical events occurring throughout fruit development and ripening. In this study, we investigated the major physiological (ethylene production and respiration) and biochemical changes (related to sugar and malic acid content as well as antioxidant metabolism) occurring during growth and on-tree ripening of two apple varieties ('Granny Smith' (GS) and 'Early Red One' (ERO)) with known differences in their postharvest behaviour, mainly firmness loss and susceptibility to superficial scald. Our results demonstrate that the higher storability and the limited loss of firmness of 'GS' fruit was associated to a higher acid content, mainly malic acid, that seemed to be regulated already at fruit set (20 DAFB).

Effect of and Allelic Variants on Grain Number and Thousand Kernel Weight of Durum Wheat and Their Impact on Final Grain Yield.

The main yield components in durum wheat are grain number per unit area (GN) and thousand kernel weight (TKW), both of which are affected by environmental conditions. The most critical developmental stage for their determination is flowering time, which partly depends on photoperiod sensitivity genes at loci. Fifteen field experiments, involving 23 spring durum wheat genotypes containing all known allelic variants at the PHOTOPERIOD RESPONSE LOCUS ( and ) were carried out at three sites at latitudes ranging from 41° to 27° N (Spain, Mexico-north, and Mexico-south, the latter in spring planting).

Optimizing Winter Wheat Resilience to Climate Change in Rain Fed Crop Systems of Turkey and Iran.

Erratic weather patterns associated with increased temperatures and decreasing rainfall pose unique challenges for wheat breeders playing a key part in the fight to ensure global food security. Within rain fed winter wheat areas of Turkey and Iran, unusual weather patterns may prevent attaining maximum potential increases in winter wheat genetic gains. This is primarily related to the fact that the yield ranking of tested genotypes may change from one year to the next.

Durum Wheat Landraces from East and West Regions of the Mediterranean Basin Are Genetically Distinct for Yield Components and Phenology.

Genetic diversity of durum wheat landraces is a powerful tool for the introgression of new alleles of commercial interest in breeding programs. In a previous study, our team structured a collection of 172 durum wheat landraces from 21 Mediterranean countries in four genetic populations related to their geographical origin: east Mediterranean (17), east Balkan and Turkey (23), west Balkan and Egypt (25), and West Mediterranean (73), leaving 34 genotypes as admixed, and association mapping was carried out for important agronomic traits. Using a subset of this collection, the current study identified 23 marker alleles with a differential frequency in landraces from east and west regions of the Mediterranean Basin, which affected important agronomic traits.

Valve morphogenesis in Diploneis smithii (Bacillariophyta).

Diploneis species have perhaps the most complex valve structure among pennate diatoms. The development of this structure was studied in Diploneis smithii and begins with the formation of a primary band, which then develops secondary arms at both poles and the center, as in the classic Chiappino-Volcani model of raphid diatom ontogeny. Spine-like projections grow out from the primary band and secondary arms to establish the transapical ribs (virgae) of the mature valve and themselves develop spines, which are spaced first oppositely and then alternately and fuse with each other to delimit the stria pores.

Modeling ecotoxicity impacts in vineyard production: Addressing spatial differentiation for copper fungicides.

Application of plant protection products (PPP) is a fundamental practice for viticulture. Life Cycle Assessment (LCA) has proved to be a useful tool to assess the environmental performance of agricultural production, where including toxicity-related impacts for PPP use is still associated with methodological limitations, especially for inorganic (i.e.

Dissecting the old Mediterranean durum wheat genetic architecture for phenology, biomass and yield formation by association mapping and QTL meta-analysis.

Association mapping was used to identify genome regions affecting yield formation, crop phenology and crop biomass in a collection of 172 durum wheat landraces representative of the genetic diversity of ancient local durum varieties from the Mediterranean Basin. The collection was genotyped with 1,149 DArT markers and phenotyped in Spanish northern and southern locations during three years. A total of 245 significant marker trait associations (MTAs) (P<0.

Biochemical and physiological changes during fruit development and ripening of two sweet cherry varieties with different levels of cracking tolerance.

The aim of this study was to investigate the biochemical and metabolic changes, related to oxidative stress, ethylene and respiration, cell wall modification and primary metabolism, between a high ('Prime Giant') and a low ('Cristalina') cracking susceptible sweet cherry cultivar during growth and ripening. While cherries are referred as a non-climacteric fruit, our results show that an increase of endogenous ethylene production at earlier fruit developmental stages is parallel to colour development and softening during growth. Higher cracking susceptibility was clearly associated to a higher fruit growth rate and accompanied by an increase net CO and ethylene production, on a cherry basis, leading to an enhanced accumulation of oxidative stress markers (i.

Tyramine and histamine risk assessment related to consumption of dry fermented sausages by the Spanish population.

Tyramine and histamine are the main dietary bioactive amines related to acute adverse health effects. Dry fermented sausages can easily accumulate high levels of these hazards and are frequently consumed in Spain. The present work aims to assess the exposure to tyramine and histamine from the consumption of dry fermented sausages by the Spanish population and to assess the risk to suffer acute health effects from this exposure.

Genetic Diversity and Association Mapping for Agromorphological and Grain Quality Traits of a Structured Collection of Durum Wheat Landraces Including subsp. durum, turgidum and diccocon.

Association mapping was performed for 18 agromorphological and grain quality traits in a set of 183 Spanish landraces, including subspecies durum, turgidum and dicoccon, genotyped with 749 DArT (Diversity Array Technology) markers. Large genetic and phenotypic variability was detected, being the level of diversity among the chromosomes and genomes heterogeneous, and sometimes complementary, among subspecies. Overall, 356 were monomorphic in at least one subspecies, mainly in dicoccon, and some of them coincidental between subspecies, especially between turgidum and dicoccon.

Multiresidue analysis of cephalosporin antibiotics in bovine milk based on molecularly imprinted polymer extraction followed by liquid chromatography-tandem mass spectrometry.

This work reports the preparation of molecularly imprinted polymers (MIPs) selective to cephalosporin (CF) antibiotics, and their application as molecularly imprinted solid-phase extraction (MISPE) sorbents for the determination of these antimicrobials in milk samples. Several functional monomers and cross-linkers have been screened to select the best combination that provides high selectivity for the simultaneous multiresidue extraction of cefthiofur (THIO), cefazolin (AZO), cefquinome (QUI), cephapirin (API), cephalexin (ALE) and cephalonium (ALO) from the samples. The novel MIPs were prepared by a non-covalent imprinting approach in the form of spherical microparticles using the synthetic surrogate molecule sodium 7-(2-biphenylylcarboxamido)-3-methyl-3-cepheme-4-carboxylate, N-3,5-bis(trifluoromethyl)phenyl-N'-4-vinylphenyl urea (VPU) as functional monomer, and divinylbenzene (DVB) as crosslinking agent in a 1:2:20 molar ratio.

Unravelling the resistance mechanisms to 2,4-D (2,4-dichlorophenoxyacetic acid) in corn poppy (Papaver rhoeas).

In southern Europe, the intensive use of 2,4-D (2,4-dichlorophenoxyacetic acid) and tribenuron-methyl in cereal crop systems has resulted in the evolution of resistant (R) corn poppy (Papaver rhoeas L.) biotypes. Experiments were conducted to elucidate (1) the resistance response to these two herbicides, (2) the cross-resistant pattern to other synthetic auxins and (3) the physiological basis of the auxin resistance in two R (F-R213 and D-R703) populations.

Epicuticular Wax in Developing Olives (Olea europaea) Is Highly Dependent upon Cultivar and Fruit Ripeness.

The epicuticular wax (EW) layer is located on the surface of most plant organs. It provides the cuticle with most of its properties and is the primary barrier against biotic and abiotic stress. Despite the importance of Olea europaea cultivation, few studies have characterized the EW covering leaves and olives, which could be involved in resistance to both infection and environmental conditions.

Distribution of Amblydromalus limonicus in northeastern Spain and diversity of phytoseiid mites (Acari: Phytoseiidae) in tomato and other vegetable crops after its introduction.

Amblydromalus limonicus (Garman and McGregor) was detected for the first time in 2011 on tomatoes of several locations of the northeastern Spain. During 2012 and 2013 samplings on tomato crop cultivars in the two provinces of Catalonia where the species was found were carried out. The goals of the study were to know the range of spread of the species in these two provinces, its abundance in tomato cultivars, non-crop vegetation among them, in the different parts of the tomato plant and in some other vegetable crops.