Isolation of a Novel Low-Temperature-Active and Organic-Solvent-Stable Mannanase from the Intestinal Metagenome of .

The black soldier fly, , is a voracious scavenger of various organic materials; therefore, it could be exploited as a biological system for processing daily food waste. In order to survey novel hydrolytic enzymes, we constructed a fosmid metagenome library using unculturable intestinal microorganisms from . Through functional screening of the library on carboxymethyl cellulose plates, we identified a fosmid clone, the product of which displayed hydrolytic activity.

Genome-Wide Characterization of the Heat Shock Transcription Factor Gene Family in Reveals Promising Candidates for Heat Tolerance.

Heat stress transcription factors (HSFs) play a critical role in orchestrating cellular responses to elevated temperatures and various stress conditions. While extensively studied in model plants, the gene family in remains unexplored, despite the availability of its sequenced genome. In this study, we employed bioinformatics approaches to identify 21 genes within the genome, revealing their uneven distribution across chromosomes.

Genome-Wide Identification and Expression Analysis of Gene Family in the Filling Stage of Wheat ( L.).

The gene family is a highly conserved transcription factor that plays a crucial role in regulating plant growth, development, and responses to various stresses. Despite extensive studies in multiple plants, there has been a dearth of focused and systematic analysis on NF-YA genes in wheat grains. In this study, we carried out a comprehensive bioinformatics analysis of the gene family in wheat, using the latest genomic data from the Chinese Spring.

In Vitro Effect of Estrogen and Progesterone on Cytogenetic Profile of Uterine Leiomyomas.

In the present study, we aimed to investigate intratumoral karyotype diversity as well as the estrogen/progesterone effect on the cytogenetic profile of uterine leiomyomas (ULs). A total of 15 UL samples obtained from 15 patients were cultured in the media supplemented with estrogen and/or progesterone and without adding hormones. Conventional cytogenetic analysis of culture samples revealed clonal chromosomal abnormalities in 11 out of 15 ULs.

Identification of Gene Expression, Evolution, and Potential Functional Sites in Horn Development of Sheep.

The growth and development of horns are primarily controlled by the skin. The gene is crucial for epidermal barrier function and may have a significant impact on horn growth. The purpose of this study was to investigate the expression of across different sheep breeds and tissues by utilizing RNA sequencing.

Unusual Genomic and Biochemical Features of sp. nov-A Novel Bacterial Species Isolated from Anthill Soil.

The black garden ant () is a widely distributed species across Europe, North America, and North Africa, playing a pivotal role in ecological processes within its diverse habitats. However, the microbiome associated with remains poorly investigated. In the present study, we isolated a novel species, , from the soil of the anthill.

Molecular Identification of Species in Ticks Infesting Hedgehogs ( and ) in North-Western Poland.

The western European hedgehog () and the northern white-breasted hedgehog () are natural hosts of the tick , the vector of tick-borne pathogens such as the bacteria responsible for Lyme disease. The aim of this study was to identify these pathogens in ticks collected from hedgehogs in northwestern Poland and to assess their genetic diversity by molecular analysis of the detected pathogens based on the gene and the intergenic spacer. Among 101 hedgehogs examined, 737 ticks were found on 56 (55.

Mechanical and Energy Evolution Characteristics of Fractured Sandstone Materials: A True Triaxial Experimental Study.

To investigate the mechanical and energy evolution characteristics of fractured rock under true triaxial stresses, true triaxial strength compression experiments on fractured sandstone were conducted with varying crack lengths and widths. The results indicate that under true triaxial stresses, the peak stress of the rock exhibits a gradual decline with an increase in crack length and width. Meanwhile, crack initiation stress and crack damage stress of fractured sandstone also demonstrate a declining trend overall, and the influence of crack length on the characteristic stress (crack initiation stress and crack damage stress) of sandstone is more pronounced than that of crack width.

Effect of Temperature and Stress on Creep Behavior of (TiB + TiC + YO)/α-Ti Composite.

In this study, a (TiB + TiC + YO)/α-Ti composite was prepared by induction skull melting to investigate its creep behavior and microstructure evolution under different temperatures and stresses. The results show that the microstructure of the composite in the as-cast state is a basket-weave structure, and the main phase composition is α lamella, containing a small amount of β phase and equiaxed α phase. The creep life of the composite decreases significantly when the temperature is increased from 650 °C to 700 °C, and the steady-state creep rate is increased by 1 to 2 orders of magnitude.

Crystal Plasticity Simulation of Cyclic Behaviors of AZ31B Magnesium Alloys via a Modified Dislocation-Twinning-Detwinning Model.

In this study, a probabilistic model within the dislotwin constitutive framework of DAMASK (the Düsseldorf Advanced Material Simulation Kit) was established to describe the cyclic loading behaviors of AZ31B magnesium alloys. Considering the detwinning procedure within the twinned region, this newly developed dislocation-twinning-detwinning model was employed to accurately simulate stress-strain behaviors of AZ31B magnesium alloys throughout tension-compression-tension (T-C-T) cycle loading. The investigations revealed that the reduction in yield stress during the reverse loading process was attributed to the active operation of twinning and detwinning modes.

Non-Optic Glioma-like Lesions in Adult Neurofibromatosis Type 1 Patients.

Physicians face clinical dilemmas in the diagnosis of non-optic intraparenchymal lesions on MRI brain scans of patients with neurofibromatosis type 1. As the incidence and evolution of these lesions into adulthood remain unclear, we conducted a retrospective study on this topic. All adult neurofibromatosis type 1 patients who had at least one MRI brain scan in our center were selected for this study.

Wood-Inhabiting Nematode, sp. n. (Nematoda: Aphelenchoididae) from David Elm, with Molecular Phylogeny of the Genus Based on Partial Mitochondrial Genomes.

A new nematode species, sp. n. is described in the bark beetle-elm tree association ( and var.

Pyroxsulam Resistance in : An Emerging Challenge in Crop Protection.

, a prevalent weed in Czech winter wheat fields, has developed resistance to ALS-inhibiting herbicides due to their frequent use. This study reports a biotype of resistant to pyroxsulam, with cross and multiple resistance to iodosulfuron, propoxycarbazone, pinoxaden, and chlortoluron. Dose-response experiments revealed high resistance of both R1 and R2 biotypes to pyroxsulam, with resistance factors (RF) of 6.

Combined Effects of Microgravity and Chronic Low-Dose Gamma Radiation on Microgreens.

Plants in space face unique challenges, including chronic ionizing radiation and reduced gravity, which affect their growth and functionality. Understanding these impacts is essential to determine the cultivation conditions and protective shielding needs in future space greenhouses. While certain doses of ionizing radiation may enhance crop yield and quality, providing "functional food" rich in bioactive compounds, to support astronaut health, the combined effects of radiation and reduced gravity are still unclear, with potential additive, synergistic, or antagonistic interactions.

Identification of Maize Kernel Varieties Using LF-NMR Combined with Image Data: An Explainable Approach Based on Machine Learning.

The precise identification of maize kernel varieties is essential for germplasm resource management, genetic diversity conservation, and the optimization of agricultural production. To address the need for rapid and non-destructive variety identification, this study developed a novel interpretable machine learning approach that integrates low-field nuclear magnetic resonance (LF-NMR) with morphological image features through an optimized support vector machine (SVM) framework. First, LF-NMR signals were obtained from eleven maize kernel varieties, and ten key features were extracted from the transverse relaxation decay curves.

Phytochemical Characterization and Biological Activity of Two Species from Guinea-Bissau.

The dried leaves of A.Rich. and Planch.

In Situ Synthesis of Ternary Ni-Fe-Mo Nanosheet Arrays for OER in Water Electrolysis.

Water electrolysis is a promising path to the industrialization development of hydrogen energy. The exploitation of high-efficiency and inexpensive catalysts become important to the mass use of water decomposition. Ni-based nanomaterials have exhibited great potential for the catalysis of water splitting, which have attracted the attention of researchers around the world.

Electromigration Techniques in the Analysis of Selected Cosmetic Ingredients: A Review.

The cosmetics industry is one of the fastest-growing sectors worldwide. The dynamic evolution of this industry results in an increasingly diverse range of products containing various active ingredients. Ensuring the quality of these products is crucial for consumer safety, necessitating the use of advanced analytical methods and adherence to legal regulations.

Identification of Two Common Bottlenose Dolphin () Ecotypes in the Guadeloupe Archipelago, Eastern Caribbean.

The common bottlenose dolphin () exhibits significant intraspecific diversity globally, with distinct ecotypes identified in various regions. In the Guadeloupe archipelago, the citizen science NGO OMMAG has been monitoring these dolphins for over a decade, documenting two distinct morphotypes. This study investigates whether these morphotypes represent coastal and oceanic ecotypes, which have not been previously identified in the region.

Genome-Wide Identification and Expression Analysis of the Gene Family in Bivalve in Response to Acute Hypersaline Stress.

The razor clam , a significant marine bivalve species, inhabits estuaries and encounters salinity stress. Despite its commercial importance, there is limited understanding of its adaptive mechanisms to high salinity. Aldehyde dehydrogenases (ALDHs), which belong to the NAD(P)-dependent superfamily, play a crucial role in stress resilience by participating in catabolic and anabolic pathways, such as carnitine synthesis, glycolysis, and amino acid metabolism.

Exploitation of Key Regulatory Modules and Genes for High-Salt Adaptation in Schizothoracine by Weighted Gene Co-Expression Network Analysis.

Schizothoracine fishes in saltwater lakes of the Tibetan Plateau are important models for studying the evolution and uplift of the Tibetan Plateau. Examining their adaptation to the high-salt environment is interesting. In this study, we first assembled the RNA-Seq data of each tissue of , , and from Qinghai Lake, Selincuo Lake, and Namtso Lake, respectively, obtained by the group previously.

Links Between Variation in Movement-Based Visual Signals and Social Communication Complexity in an Asian Agamid Lizard .

The social complexity hypothesis suggests that complex social interactions drive the evolution of sophisticated communicative signals. While the relationship between social communication and the complexity of sound and color signals has been extensively studied, the correlation between social communication and movement-based visual signal complexity remains underexplored. In this study, we selected the Asian agamid lizard, , as our model system.

Evolutionary dynamics of mitochondrial genomes and intracellular transfers among diploid and allopolyploid cotton species.

Background: Plant mitochondrial genomes (mitogenomes) exhibit extensive structural variation yet extremely low nucleotide mutation rates, phenomena that remain only partially understood. The genus Gossypium, a globally important source of cotton, offers a wealth of long-read sequencing resources to explore mitogenome and plastome variation and dynamics accompanying the evolutionary divergence of its approximately 50 diploid and allopolyploid species.

Results: Here, we assembled 19 mitogenomes from Gossypium species, representing all genome groups (diploids A through G, K, and the allopolyploids AD) based on a uniformly applied strategy.

Elucidation of genetic diversity in hawthorn (Crataegus azarolus L.) accessions naturally growing in the Eastern Mediterranean region of Türkiye using morphological, pomological, and inter simple sequence repeat markers.

Background: Türkiye hosts many important fruit species due to its geographical location and ecology. Hawthorn, which is highly beneficial for human health, is one of these significant fruit species. In the present study, 125 accessions of Crataegus azarolus L.

Spatial-temporal distribution and evolution of medical and health talents in China.

Background: In the context of public health emergencies, the presence of medical and health talents (MHT) is critically important for support in any country or region. This study aims to analyze the spatial and temporal distributions and evolution of MHT in China and propose strategies and recommendations for promoting a balanced distribution.

Methods: This research used data from 31 provinces in China to construct a multidimensional index system for measuring the agglomeration level of MHT.