Genotoxicity of radiofrequency electromagnetic fields on mammalian cells in vitro: A systematic review with narrative synthesis.

Background: Over the last decades, great concern has been raised about possible adverse effects to human health due to exposures to radiofrequency electromagnetic fields (RF-EMF, 100 kHz - 300 GHz) emitted by wireless communication technologies. In 2011 the International Agency for Research on Cancer classified RF-EMF as possibly carcinogenic to humans, highlighting that the evidence was weak and far from conclusive. Updated systematic reviews of the scientific literature on this topic are lacking, especially for mechanistic studies.

Protective effect of radiofrequency exposure against menadione-induced oxidative DNA damage in human neuroblastoma cells: The role of exposure duration and investigation on key molecular targets.

In our previous studies, we demonstrated that 20 h pre-exposure of SH-SY5Y human neuroblastoma cells to 1950 MHz, UMTS signal, at specific absorption rate of 0.3 and 1.25 W/kg, was able to reduce the oxidative DNA damage induced by a subsequent treatment with menadione in the alkaline comet assay while not inducing genotoxicity per se.

Combined Earth observations reveal the sequence of conditions leading to a large algal bloom in Lake Geneva.

Freshwater algae exhibit complex dynamics, particularly in meso-oligotrophic lakes with sudden and dramatic increases in algal biomass following long periods of low background concentration. While the fundamental prerequisites for algal blooms, namely light and nutrient availability, are well-known, their specific causation involves an intricate chain of conditions. Here we examine a recent massive Uroglena bloom in Lake Geneva (Switzerland/France).

The effect of exposure to radiofrequency LTE signal and coexposure to mitomycin-C in Chinese hamster lung fibroblast V79 cells.

This study aims to investigate the cellular effects of radiofrequency exposure, 1950 MHz, long-term evolution (LTE) signal, administered alone and in combination with mitomycin-C (MMC), a well-known cytotoxic agent. Chinese hamster lung fibroblast (V79) cells were exposed/sham exposed in a waveguide-based system under strictly controlled conditions of both electromagnetic and environmental parameters, at specific absorption rate (SAR) of 0.3 and 1.

Assessment of maize nitrogen uptake from PRISMA hyperspectral data through hybrid modelling.

The spaceborne imaging spectroscopy mission (PRISMA), launched on 22 March 2019 by the Italian Space Agency, opens new opportunities in many scientific domains, including precision farming and sustainable agriculture. This new Earth Observation (EO) data stream requires new-generation approaches for the estimation of important biophysical crop variables (BVs). In this framework, this study evaluated a hybrid approach, combining the radiative transfer model PROSAIL-PRO and several machine learning (ML) regression algorithms, for the retrieval of canopy chlorophyll content (CCC) and canopy nitrogen content (CNC) from synthetic PRISMA data.

Inhibition of Autophagy Negates Radiofrequency-Induced Adaptive Response in SH-SY5Y Neuroblastoma Cells.

In the last years, radiofrequency (RF) has demonstrated that it can reduce DNA damage induced by a subsequent treatment with chemical or physical agents in different cell types, resembling the adaptive response, a phenomenon well documented in radiobiology. Such an effect has also been reported by other authors both in vitro and in vivo, and plausible hypotheses have been formulated, spanning from the perturbation of the cell redox status, to DNA repair mechanisms, and stress response machinery, as possible cellular mechanisms activated by RF pre-exposure. These mechanisms may underpin the observed phenomenon, and require deeper investigations.

Planar Optofluidic Integration of Ring Resonator and Microfluidic Channels.

We report an optofluidic hybrid silicon-polymer planar ring resonator with integrated microfluidic channels for efficient liquid delivery. The device features a planar architecture of intersecting liquid-core waveguides and microfluidic channels. A low-loss integration of microfluidic channels is accomplished by exploiting the interference pattern created by the self-imaging effect in the multimode interference-based coupler waveguides.

Machine Learning Approach to Quadratic Programming-Based Microwave Imaging for Breast Cancer Detection.

In this work, a novel technique is proposed that combines the Born iterative method, based on a quadratic programming approach, with convolutional neural networks to solve the ill-framed inverse problem coming from microwave imaging formulation in breast cancer detection. The aim is to accurately recover the permittivity of breast phantoms, these typically being strong dielectric scatterers, from the measured scattering data. Several tests were carried out, using a circular imaging configuration and breast models, to evaluate the performance of the proposed scheme, showing that the application of convolutional neural networks allows clinicians to considerably reduce the reconstruction time with an accuracy that exceeds 90% in all the performed validations.

Spectral Methods for Response Enhancement of Microwave Resonant Sensors in Continuous Non-Invasive Blood Glucose Monitoring.

In this paper, the performance of three recent algorithms for the frequency-response enhancement of microwave resonant sensors are compared. The first one, a single-step algorithm, is based on a couple of direct-inverse Fourier transforms, giving a densely sampled response as a result. The second algorithm exploits an iterative procedure to progressively restricts the frequency response.

Further Delineation of Duplications of Locus Detected in Male Patients with Varying Degrees of Intellectual Disability.

The X-linked gene encoding aristaless-related homeobox () is a bi-functional transcription factor capable of activating or repressing gene transcription, whose mutations have been found in a wide spectrum of neurodevelopmental disorders (NDDs); these include cortical malformations, paediatric epilepsy, intellectual disability (ID) and autism. In addition to point mutations, duplications of the locus have been detected in male patients with ID. These rearrangements include telencephalon ultraconserved enhancers, whose structural alterations can interfere with the control of expression in the developing brain.

Radiofrequency Electromagnetic Field Exposure and Apoptosis: A Scoping Review of In Vitro Studies on Mammalian Cells.

In the last decades, experimental studies have been carried out to investigate the effects of radiofrequency (RF, 100 kHz-300 GHz) electromagnetic fields (EMF) exposure on the apoptotic process. As evidence-based critical evaluation of RF and apoptosis in vitro is lacking, we performed a scoping literature review with the aim of systematically mapping the research performed in this area and identifying gaps in knowledge. Eligible for inclusion were in vitro studies assessing apoptosis in mammalian cells exposed to RF-EMF, which met basic quality criteria (sham control, at least three independent experiments, appropriate dosimetry analysis and temperature monitoring).

Occupational exposure to electromagnetic fields in magnetic resonance environment: an update on regulation, exposure assessment techniques, health risk evaluation, and surveillance.

Magnetic resonance imaging (MRI) is one of the most-used diagnostic imaging methods worldwide. There are ∼50,000 MRI scanners worldwide each of which involves a minimum of five workers from different disciplines who spend their working days around MRI scanners. This review analyzes the state of the art of literature about the several aspects of the occupational exposure to electromagnetic fields (EMF) in MRI: regulations, literature studies on biological effects, and health surveillance are addressed here in detail, along with a summary of the main approaches for exposure assessment.

Dataset of Sentinel-1 surface soil moisture time series at 1 km resolution over Southern Italy.

This paper describes the specifications of the surface soil volumetric water content ( ) [m/m] product derived from Sentinel-1 (S-1) data and assessed in the study "Sentinel-1 soil moisture at 1 km resolution: a validation study" [1]. The S-1 product consists of mean and standard deviation values at 1 km spatial resolution and is expected to support applications in agriculture and hydrology as well as the Numerical Weather Prediction at regional scale [2]. The retrieval algorithm is a time series based short term change detection that is implemented in the "Soil MOisture retrieval from multi-temporal SAR data" (SMOSAR) code (v2.

Gel-Like Human Mimicking Phantoms: Realization Procedure, Dielectric Characterization and Experimental Validations on Microwave Wearable Body Sensors.

A simple and low-cost procedure for gel-like time-durable biological phantoms is presented in this work. Easily accessible materials are adopted, which are able to provide a flexible and controllable method to rapidly realize different kind of tissues. The proposed technique is applied to fabricate various tissue-mimicking phantoms, namely skin, muscle, blood and fat.

Hot moments and hotspots of cyanobacteria hyperblooms in the Curonian Lagoon (SE Baltic Sea) revealed via remote sensing-based retrospective analysis.

A temporally and spatially detailed historical (1985-2018) analysis of cyanobacteria blooms was performed in the Curonian Lagoon (Lithuania, Russia), the largest coastal lagoon in the Baltic Sea. Satellite data allowed the mapping of cyanobacteria surface accumulations, so-called "scums", and of chlorophyll-a concentration. The 34-year time series shows a tendency towards later occurrence (October-November) of the cyanobacteria scum presence, whereas the period of its onset (June-July) remains relatively constant.

SAR image wave spectra to retrieve the thickness of grease-pancake sea ice using viscous wave propagation models.

Young sea ice composed of grease and pancake ice (GPI), as well as thin floes, considered to be the most common form of sea ice fringing Antarctica, is now becoming the "new normal" also in the Arctic. A study of the rheological properties of GPI is carried out by comparing the predictions of two viscous wave propagation models: the Keller model and the close-packing (CP) model, with the observed wave attenuation obtained by SAR image techniques. In order to fit observations, it is shown that describing GPI as a viscous medium requires the adoption of an ice viscosity which increases with the ice thickness.

Genotoxicity of radiofrequency electromagnetic fields: Protocol for a systematic review of in vitro studies.

Background: Exposure to radiofrequency electromagnetic fields (RF-EMF, 100 kHz - 300 GHz) emitted by wireless communication technologies is pervasive and ubiquitous. Concern has been raised about possible adverse effects to human health. In 2011 the International Agency for Research on Cancer has classified RF-EMF as possibly carcinogenic to humans, highlighting that the evidence is weak and far from conclusive.

Special Issue "Synthetic Aperture Radar (SAR) Techniques and Applications".

This editorial of the special issue titled "Synthetic Aperture Radar (SAR) Techniques and Applications", reviews the nineteen papers selected for publication. The proposed studies investigate different aspects of SAR processing including signal modelling, simulation, image analysis, as well as some examples of applications. The papers are grouped according to homogeneous subjects, then objectives and methods are summarised, and the more relevant results are commented.

Water runoff harvesting systems for restoration of degraded rangelands: A review of challenges and opportunities.

Mismanagement of rangelands worldwide has accelerated processes of overland flow and soil erosion, resulting in extensive land degradation. Wherever self-restoration processes of degraded rangelands are hindered or negated, active recovery efforts, coupled with livestock pressure management, might be needed. The objective of this review paper is to provide land managers and environmental planners with applied and practical knowledge on advantages and disadvantages of the main methodologies and practices for runoff harvesting in rangelands.

Treatment with 3-Aminobenzamide Negates the Radiofrequency-Induced Adaptive Response in Two Cell Models.

In previous investigations, we demonstrated that pre-exposure of different cell cultures to radiofrequency fields can reduce the damage induced by genotoxic agents, an effect resembling the so-called adaptive response. In this study, we pre-exposed human peripheral blood lymphocytes and Chinese hamster lung fibroblast cell line to 1950 MHz, UMTS (Universal Mobile Telecommunication System) signal, for 20 h, and then treated cultures with Mitomycin-C. After confirming the induction of an adaptive response in terms of the reduction of micronuclei formation, we observed that such a response was negated by treatments with 3-aminobenzamide.

Protective effect of 1950 MHz electromagnetic field in human neuroblastoma cells challenged with menadione.

This study aims to assess whether a 1950 MHz radiofrequency (RF) electromagnetic field could protect human neuroblastoma SH-SY5Y cells against a subsequent treatment with menadione, a chemical agent inducing DNA damage via reactive oxygen species formation. Cells were pre-exposed for 20 h to specific absorption rate of either 0.3 or 1.

ESOPE-Equivalent Pulsing Protocols for Calcium Electroporation: An In Vitro Optimization Study on 2 Cancer Cell Models.

Reversible electroporation is used to increase the uptake of chemotherapeutic drugs in local tumor treatment (electrochemotherapy) by applying the pulsing protocol (8 rectangular pulses, 1000 V/cm, 100 µs) standardized in the framework of the European Standard Operating Procedure on Electrochemotherapy multicenter trial. Currently, new electrochemotherapy strategies are under development to extend its applicability to tumors with different histology. Electrical parameters and drug type are critical factors.

Exposure Assessment and Biomonitoring of Workers in Magnetic Resonance Environment: An Exploratory Study.

Magnetic resonance imaging (MRI) has evolved rapidly over the past few decades as one of the most flexible tools in medical research and diagnostic imaging. MRI facilities are important sources of multiple exposure to electromagnetic fields for both patients and health-care staff, due to the presence of electromagnetic fields of multiple frequency ranges, different temporal variations, and field strengths. Due to the increasing use and technological advancements of MRI systems, clearer insights into exposure assessment and a better understanding of possible harmful effects due to long-term exposures are highly needed.

Cellular Response to ELF-MF and Heat: Evidence for a Common Involvement of Heat Shock Proteins?

It has been shown that magnetic fields in the extremely low frequency range (ELF-MF) can act as a stressor in various or systems, at flux density levels below those inducing excitation of nerve and muscle cells, which are setting the limits used by most generally accepted exposure guidelines, such as the ones published by the International Commission on Non-Ionizing Radiation Protection. In response to a variety of physiological and environmental factors, including heat, cells activate an ancient signaling pathway leading to the transient expression of heat shock proteins (HSPs), which exhibit sophisticated protection mechanisms. A number of studies suggest that also ELF-MF exposure can activate the cellular stress response and cause increased HSPs expression, both on the mRNA and the protein levels.