56 results match your criteria: "Institute for Chemotherapy[Affiliation]"
Mol Cell Endocrinol
February 2004
Institute for Chemotherapy, Microbial Chemistry Research Foundation, Numazu, Shizuoka, Japan.
Androgen receptor (AR) is a ligand-activated transcription factor that requires androgen binding to initiate a series of molecular events leading to specific gene activation. AR has been suggested to form an antiparallel homodimer based on the characteristics of high affinity interaction between the amino (N) and carboxyl (C) termini of it. Recently, it is suggested that AR N-to-C interaction is critical for the ability of this receptor to up-regulate the transcription of androgen-responsive genes, and may be a new target for treatment of prostate cancer (PCa).
View Article and Find Full Text PDFJ Antibiot (Tokyo)
August 2003
Institute for Chemotherapy, Microbial Chemistry Research Foundation, 18-24 Miyamoto, Numazu-shi, Shizuoka 410-0301, Japan.
Biochem Biophys Res Commun
July 2003
Institute for Chemotherapy, Microbial Chemistry Research Foundation, 18-24 Miyamoto, Numazu-shi, 410-0301, Shizuoka-ken, Japan.
Imbalances in the epithelial-stromal interactions are important in the pathogenesis of prostate cancer. However, we know little about androgenic regulation in the stroma of prostate cancer. We examined the cancer-stromal interaction paying attention to androgen responsiveness of stromal side.
View Article and Find Full Text PDFJ Antibiot (Tokyo)
March 2003
Institute for Chemotherapy, M.C.R.F., 18-24 Miyamoto, Numazu-shi, Shizuoka, 410-0301, Japan.
ICM0201 (1), a new inhibitor of murine osteoclastogenesis in culture was isolated from a fermentation broth of Cunninghamella sp. F-1490. The structure of ICM0201 was determined to be (3S,10aR)-3,4a-dihydroxy-2,3,4,4a-tetrahydro-2H-pyrano[3,2-b]benzo[e]morpholine-9-carboxylic acid by spectroscopic analyses and chemical studies.
View Article and Find Full Text PDFJ Antibiot (Tokyo)
March 2003
Institute for Chemotherapy, M. C. R. F., 18-24 Miyamoto, Numazu-shi, Shizuoka, 410-0301, Japan.
In the course of screening for inhibitors of osteoclastogenesis, a new substance designated as ICM0201 was isolated from a fermentation broth of Cunninghamella sp. F-1490. ICM0201 inhibited the formation of osteoclasts in mouse bone marrow cells with an IC50 value of 0.
View Article and Find Full Text PDFKidney Int
April 2003
Microbial Chemistry Research Foundation, Institute for Chemotherapy, Numazu, Shizuoka, Japan.
Background: Depolymerized holothurian glycosaminoglycan (DHG) is a new agent with anticoagulant properties quite different from those of unfractionated heparin (UFH) and low-molecular-weight heparin (LMWH) in terms of antithrombin III-dependency, and exerts an antithrombotic effect with less bleeding than UFH and LMWH in vivo. In this study, the anticoagulant and hemorrhagic effects of DHG were investigated on hemodialysis in a dog model of renal failure and compared with those of UFH, LMWH, and nafamostat mesilate (FUT).
Methods: The dog renal failure model was prepared by 7/8 renal artery ligation.
Int Immunopharmacol
February 2003
Institute for Chemotherapy, Microbial Chemistry Research Foundation, 18-24 Miyamoto, Numazu, Shizuoka 410-0301, Japan.
Selective augmentation of natural killer (NK) cells can suppress tumor metastasis, but molecular targets for NK cell activation have not been identified. We report here that cytostatin (CTS), a novel specific inhibitor of protein phosphatase (PP) 2A, can inhibit B16 melanoma pulmonary metastasis by the expansion and activation of NK cells. CTS administration in vivo increased mRNA expression of Flt-3 ligand, one of NK-generating cytokines, in bone marrow cells.
View Article and Find Full Text PDFBiochem Biophys Res Commun
October 2002
Institute for Chemotherapy, Microbial Chemistry Research Foundation, 18-24 Miyamoto, Numazu-shi, Shizuoka-ken 410-0301, Japan.
Hygrolidin family antibiotics showed selective cytotoxicity against both cyclin E- and cyclin A-overexpressing cells. Among them, hygrolidin was the most potent and inhibited growth of solid tumor-derived cell lines such as DLD-1 human colon cancer cells efficiently more than that of hematopoietic tumor cells and normal fibroblasts. FACS analysis revealed that hygrolidin increased cells in G1 and S phases in DLD-1 cells.
View Article and Find Full Text PDFJ Antibiot (Tokyo)
June 2002
Institute for Chemotherapy, Microbial Chemistry Research Foundation, 18-24 Miyamoto, Numazu-shi, Shizuoka 410-0301, Japan.
J Biol Chem
August 2002
Institute for Chemotherapy, Microbial Chemistry Research Foundation, 18-24 Miyamoto, Numazu-shi, Shizuoka 410-0301, Japan.
15-Deoxyspergualin (DSG) strongly inhibited growth of mouse EL-4 lymphoma cells in vitro and in vivo. It significantly prolonged the survival days of EL-4-transplanted mice. In vitro study revealed that its antiproliferative effect appeared only after 2 days of treatment.
View Article and Find Full Text PDFInt J Cancer
August 2001
Institute for Chemotherapy, M.C.R.F., Numazu, Shizuoka, Japan.
Recent studies have shown that integrin alpha v beta 3, a receptor for vitronectin, plays an important role in tumor-induced angiogenesis and tumor growth and that antagonists of alpha v beta 3 inhibit angiogenic processes including endothelial cell adhesion and migration. On the other hand, most inhibitors of integrin alpha v beta 3 are peptide antagonists that include the Arg-Gly-Asp (RGD) motif. We therefore reasoned that non-peptide inhibitors of endothelial cell adhesion to vitronectin might be useful for inhibition of tumor angiogenesis in vivo.
View Article and Find Full Text PDFJ Antibiot (Tokyo)
December 2000
Institute for Chemotherapy, MCRF, Numazu-shi, Shizuoka, Japan.
J Antibiot (Tokyo)
July 2000
Institute for Chemotherapy, M.C.R.F., Numazu-shi, Shizuoka, Japan.
15-Deoxyspergualin (DSG) inhibited growth of mouse EL-4 lymphoma cells with an IC50 0.02 microg/ml. Even though the cells were treated with DSG for only 4 hours and then washed, the antiproliferative effect lasted long with an IC50 0.
View Article and Find Full Text PDFJ Antibiot (Tokyo)
September 1999
Institute for Chemotherapy, MCRF, Shizuoka, Japan.
IC202B (1) and C (2) were isolated from the culture filtrate of Streptoalloteichus sp. 1454-19. The structures were elucidated by various NMR spectral analyses including 1H-15N HMBC and FAB-MS experiments.
View Article and Find Full Text PDFBiochim Biophys Acta
November 1999
Institute for Chemotherapy, M.C.R.F., 18-24 Miyamoto, Numazu-shi, Shizuoka, Japan.
Cytostatin, which is isolated from a microbial cultured broth as a low molecular weight inhibitor of cell adhesion to extracellular matrix (ECM), has anti-metastatic activity against B16 melanoma cells in vivo. In this study, we examined a target of cytostatin inhibiting cell adhesion to ECM. Cytostatin inhibited tyrosine phosphorylation of focal adhesion kinase (FAK) and paxillin upon B16 cell adhesion to fibronectin.
View Article and Find Full Text PDFJpn J Cancer Res
May 1999
Institute for Chemotherapy, M.C.R.F., Numazu-shi, Shizuoka.
The cell-cell interactions between tumor cells and stromal cells are considered to be important in the regulation of tumor development at primary and metastatic secondary sites. We studied the effects of various cytokines on the cell-cell interactions between androgen-dependent LNCaP or androgen-independent PC-3 human prostate cancer cell lines and normal fibroblasts using a co-culture system. Among the tested combinations of cytokines and fibroblasts, strong modulations of cytokine actions were seen in coculture with human normal fibroblasts WI-38.
View Article and Find Full Text PDFJpn J Cancer Res
February 1999
Institute for Chemotherapy, M. C. R. F., Shizuoka.
Most solid tumor cells are less sensitive to apoptosis induced by anticancer drugs than hematopoietic cancer cells. However, the mechanisms of the different responses to apoptosis in these cell types remain unknown. To explore this question, we used B16 melanoma and EL-4 lymphoma cells as solid tumor- and hematopoietic cancer-derived cell lines, and examined the effects of two apoptosis inducers, cytostatin and bactobolin, on both cell lines.
View Article and Find Full Text PDFJ Antibiot (Tokyo)
January 1999
Institute for Chemotherapy, MCRF, Shizuoka, Japan.
IC202A (1) was isolated from the culture filtrate of Streptoalloteichus sp. 1454-19. The structure of 1 was determined by spectral analysis including a variety of two-dimentional NMR and FAB-MS experiments.
View Article and Find Full Text PDFJ Antibiot (Tokyo)
January 1999
Institute for Chemotherapy, MCRF, Shizuoka, Japan.
IC202A, a new immunosuppressive compound, was isolated from the culture filtrate of Streptoalloteichus sp. 1454-19. It showed a suppressive effect on mixed lymphocyte culture reaction with an IC50 value of 3.
View Article and Find Full Text PDFTransfusion
June 1997
Institute for Chemotherapy, Georg-Speyer-Haus, Germany.
Background: Hepatitis G virus (HGV) and its strain variant, the GB agent (GBV-C) are independent isolates of a recently identified non-A through -E hepatitis virus. Prevalence in United States volunteer blood donors is 1.5 to 1.
View Article and Find Full Text PDFAnticancer Res
December 1996
Institute for Chemotherapy, M.C.R.F., Shizuoka, Japan.
The effect of conagenin (CNG) on intestinal toxicity induction by 5-fluorouracil (5-Fu) was investigated. In mice given sublethal doses of 5-Fu (50mg/kg, i.v.
View Article and Find Full Text PDFMycoses
December 1996
Institute for Chemotherapy, Pharma Research, Bayer AG, Wuppertal, FR Germany.
To verify host-species specificities of virulence of Candida albicans in experimental systemic mycoses, 10 ATCC strains of Candida albicans, were compared for their virulence in CFW1 mice and Sprague-Dawley rats. Virulence was parallel in mice and rats, four strains were avirulent (ATCC 10231, 18804, 38245, 44831), one strain had an intermediate virulence (ATCC 32354), and five strains (ATCC 10261, 44373, 44505, 62342, 90028) were highly virulent in both host species. Infection doses of 2 x 10(6) CFU per mouse and 5 x 10(6) CFU per rat were comparable with respect to mortality of animals within 10 days; this represents a 4 : 1 ratio on the basis of body weight.
View Article and Find Full Text PDFArzneimittelforschung
December 1995
Bayer AG, Institute for Chemotherapy, Wuppertal, Germany.
Minimum inhibitory concentrations (MICs) of clotrimazole (CAS 23593-75-1, Bay 5097, clo) were determined for 142 clinical Candida isolates obtained between 1992 and 1994, including the species Candida albicans (96 strains), Candida glabrata (12 strains), Candida krusei (12 strains), and Candida tropicalis (12 strains). For some of the Candida isolates of all four species, the MICs of amphotericin B and fluconazole were also determined. No MICs of clo of > 4 micrograms/ml were found for all four Candida species, the median of MICs of clo for Candida albicans being 0.
View Article and Find Full Text PDFJ Antibiot (Tokyo)
October 1995
Institute for Chemotherapy, M. C. R. F., Shizuoka, Japan.
We have employed the DNA-methylgreen binding assay as a primary screening method for identifying apoptosis inducers in microbial products. Capsimycin, toyocamycin and cytostatin affect the binding of methylgreen to DNA of FS3 cells in this test system. The effect of cytostatin on apoptosis induction was confirmed by means of the ELISA system.
View Article and Find Full Text PDFJ Antibiot (Tokyo)
June 1995
Institute for Chemotherapy, M.C.R.F., Shizuoka, Japan.