3 results match your criteria: "Institute for Biotechnical Process Design Trier University of Applied Sciences[Affiliation]"
Novel bioreactor internals for the cultivation of spore-forming fungi in pellet form.
Eng Life Sci
July 2022
Institute for Biotechnical Process Design Trier University of Applied Sciences, Environmental Campus Birkenfeld Hoppstädten-Weiersbach Germany.
Article Synopsis
- - The study developed an automated long-term fermentation process for fungi grown in pellet form to prevent problems like overgrowth in bioreactors and improve key properties of the fermentation broth, such as oxygen transfer and mixing time.
- - Instead of using continuous culture or fed-batch fermentation, a repeated-batch fermentation method was employed with special bioreactor internals called "sporulation supports" to capture and retain biomass during fermentation.
- - After harvesting the biomass, an intermediate cleaning was conducted, and the retained pellets underwent a sporulation phase to produce spores, which were then used to inoculate the next batch, ensuring consistent spore concentration and avoiding issues from undesirable fungal growth.
Purification of mRNA with oligo(dT)-functionalized magnetic particles involves a series of magnetic separations for buffer exchange and washing. Magnetic particles interact and agglomerate with each other when a magnetic field is applied, which can result in a decreased total surface area and thus a decreased yield of mRNA. In addition, agglomeration may also be caused by mRNA loading on the magnetic particles.
View Article and Find Full Text PDFApplicability of a single-use bioreactor compared to a glass bioreactor for the fermentation of filamentous fungi and evaluation of the reproducibility of growth in pellet form.
Eng Life Sci
May 2021
Institute for Biotechnical Process Design Trier University of Applied Sciences, Environmental Campus Birkenfeld Hoppstädten-Weiersbach Germany.
The implementation of single-use technologies offers several major advantages, e.g. prevention of cross-contamination, especially when spore-forming microorganisms are present.
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