Draft Genome Sequence of Ezakiella peruensis Strain M6.X2, a Human Gut Gram-Positive Anaerobic Coccus.

We report here the draft genome sequence of strain M6.X2 The draft genome is 1,672,788 bp long and harbors 1,589 predicted protein-encoding genes, including 26 antibiotic resistance genes with 1 gene encoding vancomycin resistance. The genome also exhibits 1 clustered regularly interspaced short palindromic repeat region and 333 genes acquired by horizontal gene transfer.

Stem-Loop RNA Hairpins in Giant Viruses: Invading rRNA-Like Repeats and a Template Free RNA.

We examine the hypothesis that template-free RNAs still form spontaneously, as they did at the origins of life, invade modern genomes, contribute new genetic material. Previously, analyses of RNA secondary structures suggested that some RNAs resembling ancestral (t)RNAs formed recently , other parasitic sequences cluster with rRNAs. Here positive control analyses of additional RNA secondary structures confirm ancestral and statuses of RNA grouped according to secondary structure.

Microbial Culturomics Broadens Human Vaginal Flora Diversity: Genome Sequence and Description of Prevotella lascolaii sp. nov. Isolated from a Patient with Bacterial Vaginosis.

Microbial culturomics is a new subfield of postgenomic medicine and omics biotechnology application that has broadened our awareness on bacterial diversity of the human microbiome, including the human vaginal flora bacterial diversity. Using culturomics, a new obligate anaerobic Gram-stain-negative rod-shaped bacterium designated strain khD1 was isolated in the vagina of a patient with bacterial vaginosis and characterized using taxonogenomics. The most abundant cellular fatty acids were C anteiso (36%), C (19%), and C iso (10%).

Rickettsia sibirica mongolitimonae infection in a woman travelling from Cameroon: a case report and review of the literature.

Rickettsia sibirica mongolitimonae is now a well-known cause of human rickettsial infection, with 52 reported cases, including 47 in southern Europe and one in South Africa. We report the first case of R. sibirica mongolitimonae in Central Africa, likely a sentinel case for a more common disease than originally suspected.

Draft Genome Sequence of Strain Marseille-P3344, Isolated from a Human Fecal Microbiota.

In this article, we present the draft genome sequence of strain Marseille-P3344, isolated from a human fecal sample. The genome described here is composed of 2,464,704 nucleotides, with 2,230 protein-coding genes and 76 RNA genes.

'' a new anaerobic bacterial species isolated from the milk of a healthy African mother.

We here report the main characteristics of a new anaerobic bacterial genus and species strain Marseille-P4641 (CSUR = P4641), isolated by microbial culturomics from the milk of a 35-year-old healthy lactating mother from Mali.

Noncontiguous finished genome sequence and description of gen. nov., sp. nov., a new member of the family isolated from human colon.

Strain Marseille-P2645 was isolated in a colon sample from a Frenchwoman who underwent a colonoscopy. Bacterial cells were Gram negative, non-spore forming, mobile and strictly anaerobic. The genome of strain Marseille-P2645 is 3 950 441 bp long and contains 3374 protein-coding genes.

Molecular Evidence of Rickettsia slovaca in Wild Boar Lice, in Northeastern Algeria.

Using molecular assays, Rickettsia slovaca, the agent of a spotted fever group rickettsia was assessed in 92/325 lice collected on 117 wild boars hunted in the far northeast of Algeria. Overall, 7/92 (7.61%) Haematopinus suis were found positive to R.

Rickettsial genomics and the paradigm of genome reduction associated with increased virulence.

Rickettsia species are arthropod endosymbiotic α-proteobacteria that can infect mammalian hosts during their obligate intracellular lifecycle, and cause a range of mild to severe diseases in humans. Paradoxically, during their adaptation to a bottleneck lifestyle, rickettsial genomes have undergone an evolution marked by a progressive chromosomic and plasmidic degradation resulting in a genome reduction from 1.5 to 1.

Assessment of MALDI-TOF mass spectrometry for filariae detection in Aedes aegypti mosquitoes.

Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) is an emerging tool for routine identification of bacteria, archaea and fungi. It has also been recently applied as an accurate approach for arthropod identification. Preliminary studies have shown that the MALDI-TOF MS was able to differentiate whether ticks and mosquitoes were infected or not with some bacteria and Plasmodium parasites, respectively.

gen. nov., sp. nov., a new bacterium isolated from human stool.

Strain Marseille-P3237 was isolated from a stool sample of a healthy 35-year-old Congolese pygmy female. This anaerobic, Gram-negative, non-spore-forming and non-motile coccus-shaped bacterium is a member of the order . It exhibits a 2 009 306-bp genome with a 65.

sp. nov., isolated from a stool sample of a healthy 25-year-old woman.

We report the main characteristics of strain cv3 isolated from a stool sample of a healthy 25-year-old woman.

sp. nov., a new bacterium isolated from the vaginal flora of a woman with bacterial vaginosis.

We report the isolation of a new bacterium species, strain Marseille P4006 (CSUR P4006), isolated from a vaginal sample of a 45-year-old woman with bacterial vaginosis.

Characterization of a New Ezakiella Isolated from the Human Vagina: Genome Sequence and Description of Ezakiella massiliensis sp. nov.

The study of the vaginal microbiota using the "culturomics concept" allowed us to isolate, from the vaginal swab of an asymptomatic 20-year-old woman who had sexual relations with another woman with bacterial vaginosis, an unknown Gram-positive anaerobic coccus-shaped bacterium that was designated strain Marseille-P2951 and characterized using taxono-genomics. Strain Marseille-P2951 is non-motile and non-spore forming and exhibits catalase and oxidase activities. Its 16S rRNA gene-based identification showed 98.

"" sp. nov., a new bacterial species isolated from an extremely saline soil collected from the dry lake of Ank el Djamel in Algeria.

We report here the main characteristics of "" strain S35 (CSUR = P3927), a new bacterial species within the genus, isolated from an extremely saline soil sample collected from the site of Garaet Ank Djemel in the Wilaya of Oum El Bouaghi, Algeria.

Detection of relapsing fever Borrelia spp., Bartonella spp. and Anaplasmataceae bacteria in argasid ticks in Algeria.

Background: Argasid ticks (soft ticks) are blood-feeding arthropods that can parasitize rodents, birds, humans, livestock and companion animals. Ticks of the Ornithodoros genus are known to be vectors of relapsing fever borreliosis in humans. In Algeria, little is known about relapsing fever borreliosis and other bacterial pathogens transmitted by argasid ticks.

'' gen. nov., sp. nov., a new genus within the family of isolated from a patient with vaginosis.

sp. nov. strain MV12 is the type strain of gen.

a new bacterial species isolated from the human gut.

We report the main characteristics of sp. nov., strain Marseille-P2434 (CSUR P2434), a new species within the genus This strain was isolated from the gut microbiota of a 66-year-old diabetic patient in Marseille, France.