DNA barcoding of invasive Gambusia holbrooki Girard, 1859 and Atherina boyeri Risso, 1810 inhabiting Upper Euphrates River Basin, Türkiye.

The main contributor to Türkiye's abundant freshwater fish biodiversity is its geographic location. This fauna consists of endemic, native, and non-native fish species. The introduction of Gambusia holbrooki Girard, 1859 to Lake Amik in the 1920s for the biological control of malaria was the first introduction of nonnative species to Türkiye.

Accelerating the Future of Oncology Drug Development: The Role of Consortia in the Delivery of Precision Oncology Early Phase Clinical Trials.

Purpose: Over the past 15 years, the landscape of early phase clinical trials (EPCTs) has undergone a remarkable expansion in both quantity and intricacy. The proliferation of sites, trials, sponsors, and contract research organizations has surged exponentially, marking a significant shift in research conduct. However, EPCT operations suffer from numerous inefficiencies, such as cumbersome start-up processes, which are particularly critical when drug safety and the recommended phase II dose need to be established in a timely manner.

Liquid Active Surface Growth: Explaining the Symmetry Breaking in Liquid Nanoparticles.

In our previous studies of metal nanoparticle growth, we have come to realize that the dynamic interplay between ligand passivation and metal deposition, as opposed to static facet control, is responsible for focused growth at a few active sites. In this work, we show that the same underlying principle could be applied to a very different system and explain the abnormal growth modes of liquid nanoparticles. In such a liquid active surface growth (LASG), the interplay between droplet expansion and simultaneous silica shell encapsulation gives rise to an active site of growth, which eventually becomes the long necks of nanobottles.

A review on the effects of flavan-3-ols, their metabolites, and their dietary sources on gut barrier integrity.

Impairment of gut barrier integrity is associated with the pathogenesis of gastrointestinal diseases, including inflammatory bowel disease, colorectal cancer, and coeliac disease. While many aspects of diet have been linked to improved barrier function, (poly)phenols, a broad group of bioactive phytochemicals, are of potential interest. The (poly)phenolic sub-class, flavan-3-ols, have been investigated in some detail owing to their abundance in commonly consumed foods, including grapes, tea, apples, cocoa, berries, and nuts.

Accurate Molar Mass Estimation of Polyolefin Blends Using High Temperature-size Exclusion Chromatography With Triple Detection: Insight Into Molecular Level Mixing of Polypropylene With Various Application Grades of Polyethylene.

In this study, a commercially available polypropylene homopolymer (H-PP) was blended with blow molding polyethylene (PE) grade via melt mixing using a compounding machine. The resulting blends were subjected to high-temperature size exclusion chromatography (SEC) analysis, coupled with infrared-5 (IR-5), viscometer (VISCO), and multi-angle laser light scattering (MALS) detectors. The molecular weight (MW) and MW distributions were investigated using SEC, and the exact blend compositions were evaluated using C nuclear magnetic resonance.

Observational study: effect of varying transport durations and feed withdrawal on the physiological status and health of dairy calves.

Long-distance transport and associated fasting of unweaned calves have the potential to compromise the animals' welfare. This observational study aimed to determine how transport and fasting durations impacted the physiology and health of 115 transported calves in three transport groups; IRE (n = 20, mean age 29.8d; short road transport (~ 29 h incl.

Clinical Decision Support Using Speech Signal Analysis: Systematic Scoping Review of Neurological Disorders.

Background: Digital biomarkers are increasingly used in clinical decision support for various health conditions. Speech features as digital biomarkers can offer insights into underlying physiological processes due to the complexity of speech production. This process involves respiration, phonation, articulation, and resonance, all of which rely on specific motor systems for the preparation and execution of speech.

Insights on the role of cryoprotectants in enhancing the properties of bioinks required for cryobioprinting of biological constructs.

Preservation and long-term storage of readily available cell-laden tissue-engineered products are major challenges in expanding their applications in healthcare. In recent years, there has been increasing interest in the development of off-the-shelf tissue-engineered products using the cryobioprinting approach. Here, bioinks are incorporated with cryoprotective agents (CPAs) to allow the fabrication of cryopreservable tissue constructs.

EGFP/RFP-based FRET sensors for botulinum neurotoxin A biological activity detection and methodological validation.

Background: Botulinum neurotoxin type A (BoNT/A) is the most potent and prevalent neurotoxin known to cause botulism, and is also widely used in medical and cosmetic applications. The detection of BoNT/A is of great significance for botulism diagnosis and drug potency determination. Currently, the mouse bioassay (MBA) has long been the gold standard method but has disadvantages of ethical concerns, long testing duration, and high costs.

Diverse strategies utilized by coronaviruses to evade antiviral responses and suppress pyroptosis.

Viral infections trigger inflammasome-mediated caspase-1 activation. Nevertheless, limited understanding exists regarding how viruses use the active caspase-1 to evade host immune response. Here, we use porcine epidemic diarrhea virus (PEDV) as a model of coronaviruses (CoVs) to illustrate the intricate regulation of CoVs to combat IFN-I signaling and pyroptosis.

Weakening Pd─O Bonds by an Amorphous Pd Layer to Promote Electrocatalysis.

Construction of core-shell structured electrocatalysts with a thin noble metal shell is an effective strategy for lowering the usage of the noble metal and improving electrocatalytic properties because of the structure-induced geometric and electronic effects. Here, the synthesis of a novel core-shell structured nanocatalyst consisting of a thin amorphous Pd shell and a crystalline PdCu core and its significantly improved electrocatalytic properties for both formic acid oxidation and oxygen reduction reactions are shown. The electrocatalyst exhibits 4.

Assessing blue carbon in mangrove ecosystems of Seychelles.

Mangrove forests play an important role in climate change mitigation and adaptation, globally recognized as natural climate solution. The protection and restoration of mangrove ecosystems are especially important to Small Island Developing States, like Seychelles, due to their vulnerability to the impacts of climate change, such as sea level rise and tropical cyclones. Therefore, it is crucial for countries like Seychelles to develop baseline information on the status of their mangrove forests to guide conservation and management actions.

Beneficial effects of ε-polylysine on growth performance, antioxidant capacity, intestinal morphology, and gut microbiota in broilers fed a lysine-deficient diet.

This study was conducted to investigate the effects of dietary ε-polylysine supplementation on growth performance, antioxidant capacity, intestinal morphology, and gut microbiota in broilers fed a lysine-deficient diet. A total of 960 one-day-old yellow-feathered broilers were randomly allocated into six groups with eight replicates and 20 birds per replicate. Birds in the six groups were fed a lysine-deficient basal diet (-0.

Paraspinal synovial sarcoma mimicking tuberculosis: A case report and literature review.

Rationale: Synovial sarcoma (SS) is a rare and highly malignant soft tissue sarcoma. When SS occurs in atypical locations, it can present significant diagnostic challenges. We report a case of paraspinal SS initially misdiagnosed as spinal tuberculosis, highlighting the diagnostic difficulties and the importance of considering SS in the differential diagnosis.

Unraveling the genetic architecture of blueberry fruit quality traits: major loci control organic acid content while more complex genetic mechanisms control texture and sugar content.

Background: Fruit quality traits, including taste, flavor, texture, and shelf-life, have emerged as important breeding priorities in blueberry (Vaccinium corymbosum). Organic acids and sugars play crucial roles in the perception of blueberry taste/flavor, where low and high consumer liking are correlated with high organic acids and high sugars, respectively. Blueberry texture and appearance are also critical for shelf-life quality and consumers' willingness-to-pay.

Anomalous photovoltaics in Janus MoSSe monolayers.

The anomalous photovoltaic effect (APE) in polar crystals is a promising avenue for overcoming the energy conversion efficiency limits of conventional photoelectric devices utilizing p-n junction architectures. To facilitate effective photocarrier separation and enhance the APE, polar materials need to be thinned down to maximize the depolarization field. Here, we demonstrate Janus MoSSe monolayers (~0.

Preparation of thermoresponsive & enzymatically crosslinkable gelatin-gellan gum bioink: A protein-polysaccharide hydrogel for 3D bioprinting of complex soft tissues.

Developing superior bioinks present several challenges in achieving ideal properties such as biocompatibility, viscosity, degradation rates & mechanical properties which are required to make functional tissue constructs. Various attempts have been made to prepare excellent bioink compositions that are suitable to address the above challenges. Herein, a versatile combination of gelatin (GL) - gellan gum (GG) bioink was successfully formulated & the bioink 7.

Dynamic supramolecular snub cubes.

Mimicking the superstructures and properties of spherical biological encapsulants such as viral capsids and ferritin offers viable pathways to understand their chiral assemblies and functional roles in living systems. However, stereospecific assembly of artificial polyhedra with mechanical properties and guest-binding attributes akin to biological encapsulants remains a formidable challenge. Here we report the stereospecific assembly of dynamic supramolecular snub cubes from 12 helical macrocycles, which are held together by 144 weak C-H hydrogen bonds.

Effect of a single rectal fecal microbiota transplantation on clinical severity and fecal microbial communities in dogs with chronic inflammatory enteropathy.

Background: Fecal microbiota transplantation (FMT) has been advocated as a treatment for chronic enteropathy (CE) in dogs. However, so far only short-term clinical effects have been reported whereas the effect on the microbiota remains unexplored.

Hypothesis/objectives: Assess if a single FMT enema can lead to clinical improvement in dogs with CE when accompanied by presumed favorable microbiota changes.

Associations between maternal fish intake and polyunsaturated fatty acid status with childhood asthma in a high fish-eating population.

Background: Studies investigating associations between prenatal polyunsaturated fatty acid status (PUFAs), in particular the anti-inflammatory n-3 PUFAs, and the development of childhood asthma have yielded conflicting results.

Objective: To determine the associations between maternal fish intake (a rich source of the n-3 PUFAs), maternal or cord PUFAs with the prevalence of childhood asthma in a high fish-eating population.

Methods: We examined these associations between fish intake and PUFA concentrations with childhood asthma prevalence in the Seychelles Child Development Study Nutrition Cohort 2, a large observational study in a high fish-eating population.

Importance of an N-terminal structural switch in the distinction between small RNA-bound and free ARGONAUTE.

ARGONAUTE (AGO) proteins bind to small non-coding RNAs to form RNA-induced silencing complexes. In the RNA-bound state, AGO is stable while RNA-free AGO turns over rapidly. Molecular features unique to RNA-free AGO that allow its specific recognition and degradation remain unknown.

Divergent response to radio-immunotherapy is defined by intrinsic features of the tumor microenvironment.

Background: Treatment with immunotherapy can elicit varying responses across cancer types, and the mechanistic underpinnings that contribute to response vrsus progression remain poorly understood. However, to date there are few preclinical models that accurately represent these disparate disease scenarios.

Methods: Using combinatorial radio-immunotherapy consisting of PD-1 blockade, IL2Rβγ biased signaling, and OX40 agonism we were able to generate preclinical tumor models with conflicting responses, where head and neck squamous cell carcinoma (HNSCC) models respond and pancreatic ductal adenocarcinoma (PDAC) progresses.

Combined μ-XRF and XANES Track the Behavior of Pb from PM Entering Chinese Cabbage Leaves.

Atmospheric fine particulate matter (PM) is the main contributor to Pb accumulation in edible Chinese cabbage leaves in North China. PM-Pb primarily enters leaves via stomatal foliar uptake. However, how PM-Pb is transported and stored within the leaf cells of Chinese cabbage remains unclear.

Comparative Genome Analysis of Piscine : Virulence-Associated Metabolic Pathways.

Vibriosis caused by is a major problem in aquatic animals, particularly brown marble groupers (). biotype I has recently been isolated and classified into subgroups SUKU_G1, SUKU_G2, and SUKU_G3 according to the different types of virulence genes. In a previous study, we have shown that biotype I strains were classified into three subgroups according to the different types of virulence genes, which exhibited different phenotypes in terms of growth rate and virulence.