Ligand-based pharmacophore modeling, virtual screening and biological evaluation to identify novel TGR5 agonists.

Takeda G-protein-coupled receptor 5 (TGR5) is emerging as an important and promising target for the development of anti-diabetic drugs. To understand the structural characteristics of TGR5 agonists, the common feature pharmacophore models were generated and molecular docking was performed. The ligand-based virtual screening combined with pharmacophore mapping and molecular docking was performed to identify novel nonsteroidal TGR5 agonists.

Heart rate variability and heart rate monitoring of nurses using PPG and ECG signals during working condition: A pilot study.

The use of wearable photoplethysmography (PPG) technology for estimating heart rate (HR) and HR variability (HRV) in the health care system is gaining attention in recent years. However, the performance of these devices remains questionable in their ability to collect data in real working conditions for long-term monitoring. The present study aimed to examine the data collected from nurses during working hours by PPG and electrocardiography (ECG) devices.

[Applying Biomod2 for modeling of species suitable habitats:a case study of Paeonia lactiflora in China].

Paeonia lactiflora is an important medicinal resource in China. It is of great significance for the protection and cultivation of P. lactiflora resources to find the suitable habitats.

Carcinoma-associated fibroblasts promote the proliferation and metastasis of osteosarcoma by transferring exosomal LncRNA SNHG17.

Cancer-associated fibroblasts (CAFs) serve as a predominant regulator in the tumor microenvironment. However, the crosstalk between CAFs and OS cells remains mostly unclear. Recent studies explored that long non-coding RNA (LncRNAs) involved in regulating osteosarcoma (OS) formation and development, but their functions in CAFs are unknown.

The Positive Association between Subclinical Hypothyroidism and Newly-Diagnosed Hypertension Is More Explicit in Female Individuals Younger than 65.

Background: Subclinical hypothyroidism (SCH) is the most common thyroid dysfunction, and its relationship with blood pressure (BP) has been controversial. The aim of the study was to analyze the association between SCH and newly-diagnosed hypertension.

Methods: Based on data from the Thyroid disease, Iodine nutrition and Diabetes Epidemiology (TIDE) study, 49,433 euthyroid individuals and 7,719 SCH patients aged ≥18 years were enrolled.

Effects of topical oxygen therapy on chronic traumatic wounds and its impact on granulation tissue.

Objective: To evaluate the effects of topical oxygen therapy and its impacts on granulation tissue in patients with chronic traumatic wounds.

Method: A total of 112 patients with chronic traumatic wounds were randomly divided into the control group (n=56, receiving negative-pressure wound therapy) and the intervention group (n=56, receiving negative-pressure wound therapy plus topical oxygen therapy) using a random number table and they were treated continuously for 2 weeks. Then, the scores from the Pressure Ulcer Scale for Healing (PUSH), the coverage rate of granulation tissue, the severity of pain and Transcutaneous Oxygen Partial Pressure (TcPO) before and after treatment were compared between the two groups.

Bispecific T cell engagers and their synergistic tumor immunotherapy with oncolytic viruses.

Tumor immunotherapy, especially T cell based therapy, is becoming the main force in clinical tumor therapies. Bispecific T cell engager (BiTE) uses the single chain variable fragments (scFv) of two antibodies to redirect T cells to kill target cells. BiTEs for hematologic tumors has been approved for clinical use, and BiTEs for solid tumors showed therapeutic effects in clinical trials.

Identification of candidate targets for the diagnosis and treatment of atherosclerosis by bioinformatics analysis.

Atherosclerosis, a chronic inflammatory disease, is the primary cause of most cardiovascular diseases. Circular RNAs (circRNAs) were reported to serve as post-transcriptional regulators and diagnostic markers in various diseases, but the underlying correlation between circRNAs and atherosclerosis remains elusive. In this study, we downloaded the microarray dataset GSE107522 from the Gene Expression Omnibus (GEO) and identified nine differentially expressed circRNAs (DECs).

[New effect of G-protein coupled receptors on blood pressure regulation].

Hypertension is a clinical syndrome characterized by elevated systemic arterial blood pressure, which may be accompanied by functional or organic damage of heart, brain, kidney and other organs. The pathogenesis and development of hypertension are affected by genetic, environmental, epigenetic, intestinal microbiota and other factors. They are the result of multiple factors that promote the change of blood pressure level and vascular resistance.

Pharmacophore modeling and virtual screening studies for discovery of novel farnesoid X receptor (FXR) agonists.

Farnesoid X receptor (FXR) agonists would be considered as an important therapeutic strategy for several chronic liver and metabolic diseases. Here we have employed an integrated virtual screening by combining ligand-based pharmacophore mapping and molecular docking to identify novel nonsteroidal FXR agonists. Eighteen compounds were selected for FXR agonistic activity assay, and results showed five compounds exhibiting promising FXR agonistic activity.

[Differences in spatial distribution of medicinal plant resources in Yinshan region of Inner Mongolia].

Yinshan Mountains stands on the southern edge of the Inner Mongolia Plateau, which stretches 1 200 km from east to west and 50 to 100 km from north to south. The rich and varied topographic environment of the Yinshan Mountains has created a variety of vegetation floras, which also makes the species of medicinal plant resources in this area unevenly distributed. Therefore, studying the spatial distribution difference of medicinal plant resources among various banners, counties, and districts in the Yinshan area is of great significance to formulate the protection policy and promote the industry development of medicinal plant.

Highly bioavailable curcumin preparation with a co-grinding and solvent-free process.

Curcumin (Cur.) is a natural product isolated from the rhizome of with a variety of biological and pharmacological activities in food and pharmaceutical products. However, curcumin's poor solubility in water greatly limits its bioavailability and clinical applications.

Oncogenic State and Cell Identity Combinatorially Dictate the Susceptibility of Cells within Glioma Development Hierarchy to IGF1R Targeting.

Glioblastoma is the most malignant cancer in the brain and currently incurable. It is urgent to identify effective targets for this lethal disease. Inhibition of such targets should suppress the growth of cancer cells and, ideally also precancerous cells for early prevention, but minimally affect their normal counterparts.

[Study on traditional processing method of Mongolian medicine and excipient usage based on data mining].

This paper explores Mongolian medicine processing methods and the use regularity of excipient by text mining techniques. Relevant books of Mongolian medicine processing were consulted to collect data on Mongolian medicine processing methods and excipient, and select data based on processing methods and excipient noun frequency statistics. Microsoft Excel 2010 software was used for statistical analysis and mining for the usage regularity of different types of Mongolian medicinal materials in different periods.

Linc-OIP5 working as a ceRNA of miR-616 promotes PON1 expression in HUEVC cells.

Coronary atherosclerosis affects human health all over the world. PON1 was found to be associated with coronary atherosclerosis but the specific mechanism is still unclear. Non-coding RNA plays an important role in many diseases.

[Study on protective effect of vanillic acid from Astragalus membranaceus on hypertensive cardiac remodeling based on network pharmacology screen].

To identify and verify the active ingredients from Astragalus membranaceus on hypertensive cardiac remodeling based on network pharmacology and heart RNA-sequencing data. The monomers of A. membranaceus and their intervention target database were established by using network pharmacology.

Primary aldosteronism complicated by hyperparathyroidism: report of one case and literature review.

Primary aldosteronism (PA) is a category of secondary hypertension induced by inhibition of the renin-angiotensin system due to increased aldosterone secretion. Aldosterone-producing adenoma (APA) is the most common hypotype of PA. Primary hyperparathyroidism (PHPT) refers to the symptoms of increased bone resorption and increased calcium reabsorption of kidney tubules caused by parathyroid secretion and excessive synthesis of parathyroid hormone.

MiR-210 inhibits hypoxia-induced apoptosis of smooth muscle cells via targeting MEF2C.

Background: Vascular smooth muscle cell (VSMC) apoptosis plays an important role in vascular remodeling and atherosclerotic plaque instability. Growing evidence suggests that microRNAs (miRNAs) play a critical role in VSMC function, however, the underlying mechanism remains unclear.

Methods: This study used a hypoxic-induced VSMC apoptosis model.

Protective effects of Ulinastatin on oxidative stress and inflammation of rat-derived cardiomyocytes H9c2.

Ischemic heart disease (IHD) is a common clinical disease and has a younger tendency in recent years. This study focused on the role of Ulinastatin (UTI) in the anti-oxidative stress and anti-inflammatory response of cardiomyocytes. H9c2 cells were divided into control group, ischemia-anoxic group (ischemic hypoxia group) and ischemia-anoxia + UTI group (UTI group).

A series of Mn(i) photo-activated carbon monoxide-releasing molecules with benzimidazole coligands: synthesis, structural characterization, CO releasing properties and biological activity evaluation.

Five Mn(i) photo-activated carbon monoxide-releasing molecules (photo-CORMs) with benzimidazole coligands, namely [MnBr(CO)L1] (1, L1 = 2-(2-pyridyl)benzimidazole), [Mn(CO)L1(PPh)](ClO) (2), [MnBr(CO)L2] (3, L2 = 2,2'-bisbenzimidazole), [MnBr(CO)L3]·CHOH (4, L3 = 2,6-bis(benzimidazole-2'-yl)pyridine) and -[MnBr(CO)L4] (5, L4 = 2,4-bis(benzimidazole-2'-yl) pyridine) were synthesized by reactions of MnBr(CO) with complexes L1-L4, respectively, and characterized single crystal X-ray diffraction, elemental analysis, H-NMR, C-NMR, IR, UV-vis and fluorescence spectroscopy. The CO-release properties of 1-5 were investigated using the myoglobin assay and CO detection, and the results show that all of the complexes could release CO rapidly upon exposure to 365 nm UV light. Comparing their half-lives of CO release, we found that increasing the degree of unsaturation and conjugation of the ligand frame could be advantageous for prolonging the time of CO-release, and that the luminescence intensity of 1-5 could gradually be enhanced.

Effects of sleeve gastrectomy on lipid and energy metabolism in ZDF rats via PI3K/AKT pathway.

Sleeve Gastrectomy (SG), as the most effective bariatric surgery, has been using to chronically lose weight and control glucose metabolism in Type 2 diabetes mellitus patients. However, the underlining mechanism is still unclear. In this study, we performed SG on Zucker diabetes fatty (ZDF) rat and investigated visceral lipid metabolism and energy metabolism.