Effect of hepatic blood inflow occlusion without hemihepatic artery control on apoptosis of liver cells in rats.

Background/aims: To investigate the effect of hepatic blood inflow occlusion without hemihepatic artery control on the apoptosis of liver cells during ischemia-reperfusion injury in rats.

Methodology: Animal models of three hepatic vascular occlusion methods were established. Hepatic blood inflow occlusion without hemihepatic artery control (WH group), hemihepatic vascular occlusion (HH group) and Pringle maneuver (PR group) were compared.

The role of small molecular weight compounds to increase vacuolation induced by VacA toxin in vitro.

VacA is a vacuolation protein toxin secreted by Helicobacter pylori. Many compounds have been implicated in the regulation of VacA toxin activity. In this study, regulation of cell vacuolation induced by VacA was observed with the addition of glycine, glycine hydrochloride, xylitol, and taurine by neutral red dye uptake assay using gastric human epithelial cell cultures.

[Research of Zhanbra Dorje's contribution on the development of Mongolian medicine].

Zhanbra Dorje, a famous Mongolian physician, is one of the top ten Mongolian scientists. He firstly presented the illustrations for 561 kinds of Mongolian materia medica in his book, Classic Canon of Mongolian Materia Medica, which was greatly helpful to the recognition, collection, using, learning, research and identification of Mongolian materia medica. In this book, he annotated the name of Mongolian materia medica by four kinds of languages, which contributed significantly to the academic and cultural exchanges among Mongolian, Tibetan, Han and Manchu medicine.

[Function reconstruction of anterior and middle column in thoracolumbar spinal tuberculosis by one-stage anterior radical debridement].

Objective: To summarize the effect of one-stage anterior debridement of infection in function reconstruction of anterior and middle column for the treatment of thoracolumbar spinal tuberculosis.

Methods: From January 2001 to January 2007, 65 patients with thoracolumbar spinal tuberculosis were treated with one-stage anterior debridement, decompression, autogenous bone grafts and internal fixation. There were 43 males and 22 females with an average age of 40.

Reverse line blot hybridization and DNA sequencing studies of the 16S-23S rRNA gene intergenic spacer regions of five emerging pathogenic Nocardia species.

The objective of this study was to examine DNA sequence polymorphisms in the 16S-23S rRNA gene intergenic spacer (ITS) regions of five emerging pathogenic Nocardia species: Nocardia beijingensis, Nocardia blacklockiae, Nocardia thailandica, Nocardia elegans and Nocardia vinacea. A set of six isolates belonging to the species of interest and 135 isolates belonging to other Nocardia species was studied. A PCR-based reverse line blot (RLB) hybridization assay incorporating species- or intraspecies ITS rRNA gene operon-specific probes was then developed for species identification.

[Combination of intensified anti-tuberculosis with operation for treatment of thoracolumbar tuberculosis].

Objective: To evaluate the clinical significance of the combination of intensified anti-tuberculosis treatment, posterior transpedicular screw system internal fixation, intertransverse bone grafting, and anterior approach focus debridement for the treatment of thoracolumbar tuberculosis.

Methods: From January 2005 to December 2007, 20 patients with thoracolumbar tuberculosis (T10-L3) were treated. There were 13 males and 7 females aged 21-64 years old (average 44 years old).

[Detection of tumor cells in peripheral blood of patients with gastric cancer using mRNA of MAGE genes as markers].

Objective: To develop a sensitive and specific RT-PCR assay using the mRNA of MAGE-1 and MAGE-3 genes as specific tumor markers for the detection of the tumor cells in the peripheral blood of patients with gastric cancer.

Methods: Peripheral blood was obtained from 40 patients with gastric cancer and from 20 healthy volunteers. The mRNA of MAGE-1 and MAGE-3 genes in the peripheral blood mononuclear cells (PBMC) was detected by RT-PCR.

[Diagnosis and treatment of bone marrow edema syndrome of the hip and differential diagnosis from avascular necrosis of femoral head].

Objective: To observe the diagnosis and treatment of bone marrow edema syndrome and summarize its features, mechanisms and its differences from avascular necrosis of femoral head.

Methods: From 2004.1, 19 patients (12 patients were males and 7 patients were females, with a mean age of (46.

[In vitro study of expression and effects of HSP70/HSP90 in nasopharyngeal carcinoma cells after thermotherapy].

Objective: To investigate the changes and effects of HSP70/HSP90 of in nasopharyngeal carcinoma cells HNE1 after thermotherapy.

Methods: HNE1 cells were incubated at 42 degrees C for 2 h. The changes of mRNA and protein level of HSP70/HSP90 were detected by real-time PCR and western-blot at different intervals.

Protective effect of pyrroloquinoline quinone against Abeta-induced neurotoxicity in human neuroblastoma SH-SY5Y cells.

The neurotoxicity of aggregated beta-amyloid (Abeta) has been implicated as a critical cause in the pathogenesis of Alzheimer's disease (AD). It can cause neurotoxicity in AD by evoking a cascade of oxidative damage-dependent apoptosis to neurons. In the present study, we for the first time investigated the protective effect of pyrroloquinoline quinone (PQQ), an anionic, water soluble compound that acts as a redox cofactor of bacterial dehydrogenases, on Abeta-induced SH-SY5Y cytotoxicity.

[The expression of alpha-smooth muscle actin in primary cultural fibroblasts of rats].

Aim: To observe the expression of a-smooth muscle actin(a-SMA) in primary cultural fibroblasts of rats.

Methods: 12 female Wistar rats were randomly assigned into two groups, the normal group and the model group. The model group was filled with bleomycin A2 (5 mg/kg) once into the trachea.

Application of navigation template to fixation of sacral fracture using three-dimensional reconstruction and reverse engineering technique.

Objective: To provide a new method in the fixation of sacral fracture by means of three-dimensional reconstruction and reverse engineering technique.

Methods: Pelvis image data were obtained from three-dimensional CT scan in patients with sacral fracture. The data were transferred into a computer workstation.

[Studies on chemical constituents of leaves of Aquilaria sinensis].

Objective: To investigate the chemical constituents of the leaves of Aquilaria sinensis, and provide a certain of basis for the comprehensive uses of the plant of A. sinensis.

Method: The chemical constituents were isolated by various column chromatographic method.

[Expression and significance of the cyclin E in laryngeal carcinomas].

Objective: To investigate the clinicopathological significance of the cyclin E expression in laryngeal squamous cell carcinomas (LSCCs).

Method: The expression of cyclin E was detected by immunohistochemistry method in 50 cases of LSCCs, 20 cases of dysplastic squamous epithelium and 10 polyps of vocal cord.

Result: The positive expression of cyclin E was localized in the nuclei of cells.

[Clinical analysis of microsurgical carotid endarterectomy for carotid stenosis and occlusion].

Objective: To evaluate the clinical effects of carotid endarterectomy for carotid stenosis and occlusion.

Methods: From August 2005 to November 2008 moderate and severe carotid stenosis or occlusion were found in 16 patients by Doppler ultrasonography (DUS), MRA, CTA, DSA. The stenosis degree ranged from 60% to 99% in 14 patients and complete occlusion in 2 patients.

[Pedicle screw fixation and allograft bone in posterior spinal fusion for treatment of thoracolumbar vertebral fractures].

Objective: To investigate the efficacy of the pedicle screw fixation and allograft bone in posterior spinal fusion for the treatment of thoracolumbar vertebral fractures.

Methods: From September 2006 to March 2008, 105 cases of thoracolumbar vertebral fractures were treated with allograft bone in posterior spinal fusion after the pedicle screw fixation, including 75 males and 30 males aged 15-65 years (mean 37 years). The mixture which consisted of spinous process and vertebral plate sclerotin and homogeneity variant bone was used as bone graft to implant into articular process and processus transversus space or vertebral plate space.

Flow-injection chemiluminescence method for the determination of naphazoline hydrochloride and oxymetazoline hydrochloride.

A sensitive and simple flow-injection chemiluminescence (FI-CL) method, which was based on the CL intensity generated from the redoxreaction of potassium permanganate (KMnO4)-formaldehyde in vitriol (H2SO4) medium, has been developed, validated and applied for the determination of naphazoline hydrochloride and oxymetazoline hydrochloride. Besides oxidants and sensitizers, the effect of the concentration of H(2)SO(4), KMnO4 and formaldehyde was investigated. Under the optimum conditions, the linear range was 1.

[Expression of CK20 mRNA in the peripheral blood around postoperative chemotherapy in patients with gastric cancer and its clinical significance].

Objective: To investigate whether CK20 mRNA expression level could be considered as an effective molecular indicator for evaluation of chemotherapy sensitivity.

Methods: All samples of peripheral blood were taken from 31 gastric cancer patients undergone radical operation a week before postoperative chemotherapy, at the first day of chemotherapy point, and after the first cycle of chemotherapy respectively, and subjected to FQ RT-PCR assay for CK20 mRNA. The chemotherapy scheme was FOLFOX 4.

Research on Influences of Transient High IOP during LASIK on Retinal Functions and Ultrastructure.

Objectives. To study the influences of transient high intraocular pressure(IOP) during LASIK on retinal functions and ultrastructure. Methods.

[Determination of strength of femoral neck in patients with hip fracture].

Objective: To study the relationship between the hip fracture and osteoporosis of the aged.

Methods: The strength of contralateral unfractured proximal femur (Singh index) in 80 patients with hip fractures from 1998 to 2003 were collected, and the patients' age were all over 50 years. At the same time, the strength of proximal femur of 90 patients without fractures were also measured, and the figures were analyzed.

[Effect of ACBP-S on cell cycle and apoptosis in human gastric cancer cells].

Objective: To explore the impact of anti-cancer bioactive peptide-S (ACBP-S) on cell proliferation, cell cycle and apoptosis in human stomach cancer cell line MGC-803 cells.

Methods: (1) The cultured MGC-803 cells were treated with ACBP-S at various concentrations for 24, 48, 72 h, respectively. The inhibition rate of proliferation of MGC-803 cells were evaluated by MTT assay.

p73 expression and its clinical significance in colorectal cancer.

Objective: To explore p73 mRNA and p73 protein expression and their clinical significance in colorectal cancer.

Method: p73 mRNA and p73 protein expression were detected using hybridization in situ and immunohistochemical method in cancerous tissue from 60 patients of colorectal cancer and in paracancerous tissue from 23 patients among the corresponding 60 patients of colorectal cancer. Quantitative analysis was performed using Smartscape image analysis system.

Development of a new animal model of liver cirrhosis in swine.

This study aimed to develop a new experimental model of liver cirrhosis in swine by using carbon tetrachloride (CCl(4)) and ethanol. Liver cirrhosis was induced by intraperitoneal injection of CCl(4) twice a week for 9 weeks. Maize flour was the only food provided and the animals drunk a 5% alcohol-water mixture.

Bradykinin-induced blood-tumor barrier opening is mediated by tumor necrosis factor-alpha.

Bradykinin has been shown to increase the permeability of blood-tumor barrier (BTB) selectively. This study was performed to determine whether tumor necrosis factor-alpha (TNF-alpha) was involved in the regulation of this biological process. We found that the levels of TNF-alpha mRNA and heat shock factor-1 (HSF1) protein in C6 cells were markedly up-regulated by bradykinin via real-time RT-PCR and Western blot methods.