[Study of genetic polymorphism of 6 short tandem repeat loci in Nongqu Mongolian of inner Mongolia Autonomous Region in China].

Objective: To get the genotype and allele frequency distribution of 6 short tandem repeat (STR) loci VWA, FGA, PENTAE, D6S1043, D2S1772, D7S3048 in NongQu Mongolia of China.

Methods: Two hundred and ninety-three unrelated individuals from Nongqu Mongolian were investigated. Polymerase chain reaction and polyacrylamide gel electrophoresis were used.

[Identification of novel mutations in the phenylalanine hydroxylase gene of classical phenylketonuria].

Objective: To investigate the mutations of phenylalanine hydroxylase (PAH) gene in the cases of classical phenylketonuria (PKU).

Methods: The exons 3-12 of the PAH gene in 32 PKU patients from Inner Mongolia were studied by using PCR-single strand conformation polymorphism technique and DNA direct sequencing.

Results: Fourteen point mutations were identified.

[Promoting effect of hyperhomocysteinemia on vascular calcification in rats].

Aim: To explore the effect of hyperhomocysteinemia on vascular calcification and the underlying mechanism of it.

Methods: Arterial calcification of Sprague-Dawley rats was induced by vitamin D3 plus nicotine. Hyperhomocysteinemia was established by feeding high methionine diet for six weeks and was assessed b y plasma total homocysteine (tHcy) level detected by HPLC method.

[Research of the P substance on the olfactory mucous in sinusitis].

Objective: To discuss the pathogenesis of dysosphresia in the chronic sinusitis patients.

Method: Fifty-five cases with chronic sinusitis as experimental group, and 11 cases as control,mere analyzed with acidophilus monitor, and then the P substances in their olfactory mucous were detected.

Result: The positive ratio is 50.

Population studies on two native Mongolian population groups in China using STR loci.

The allelic distribution of nine short tandem repeats (STRs) loci (vWA, FGA, PentaE, D3S1358, D13S317, D5S818, D6S1043, D2S1772 and D7S3048) was examined in 286 Ximeng-Mongolian and 293 Wumeng-Mongolian unrelated individuals living in the Inner Mongolia province, PR China.

Detection of human papillomavirus-16 in ovarian malignancy.

Human papillomavirus is the causal factor for cervical cancer. However, the role of HPV infection in ovarian cancer is unclear. This study aimed to determine the presence of human papillomavirus-16 (HPV-16) in ovarian cancer tissues.

[A herbalogical study on traditional Mongolian medicine dakesha].

By herbological study and investigation, "Dekesha" used by Mongolia doctors mainly contains 17 species from 3 genus of 3 family, but "Dakesha" in Inner Mongolia Standard of Medicinal Materials only contains 2 species which are Oxytropis myriophylla and O. hirta.

[Expression and significance of TGF-beta 1 in nasal polyps].

Objective: To study the expression and significance of transforming growth factor-beta 1(TGF-beta 1) in nasal polyps.

Method: Expression of TGF-beta 1 in nasal polyps from 34 patients and middle turbinates from 30 patients with deviation of nasal septum were prospectively studied with immunohistochemistry. Each tissue section was observed under optical microscope.

[Correlation between HLA-DQA1 allele and anaphylactoid purpura in juvenile Hans residing in Inner Mongolia].

Objective: To analyze the genetic susceptibility of HLA-DQA1 allele to anaphylactoid purpura(AP)and its association with the clinical features in juvenile Hans residing in Inner Mongolia.

Methods: Seventy children with AP and ninety normal controls of Hans in Inner Mongolia were subjected to HLA-DQA1 genotyping with the use of polymerase chain reaction-sequence specific primer (PCR-SSP) technique.

Results: (1) The gene frequency of HLA-DQA1*0301 of AP group (33.

[The manufacturing of penicillin in China in 1940s].

A small modernized factory was established in early 1947, and developed into industrialized production. The manufacture of penicillin in China in the 1940s lay down the foundation for the industrialized production of penicillin in China which occupies an important position in the medical history of China.

[The relationship between loudness recruitment phenomenon and distortim product otoacoustic emission and their clinical significance].

Objective: To study the relationship between loudness recruitment and abnormal cochlear mechanism.

Methods: The basic properties of input/output (I/O) of distortion product otoacoustic emission (DPOAE) in 20 normal subjects(40 ears) and 50 cases with cochlear deafness (63 ears) with positive recruitment were studied and analyzed.

Results: Average slope of I/O at different frequencies in all deaf ears with recruitment was greater than those in the normal subjects with a statistical significance (P < 0.

[Spreading of Hua Shou's medical books in Japan].

The paper inquired into the situation of spread and influence of a distinguished physician of the Yuan dynasty, Hua Shou's medical books in Japan. Nippon editions of Hua Shou's medical books had been investigated. The conditions that Hua Shou's medical books were translated, annotated, studied by Japanese medical workers before Meiji, and the role of Hua Shou's medical books played on the medical education in Japan was also discussed.

Prevalence of Eperythrozoon spp. infection and congenital eperythrozoonosis in humans in Inner Mongolia, China.

Eperythrozoon is an obligate parasitic bacteria found in many species of animals. A large scale investigation of the prevalence of Eperythrozoon spp. in humans, was conducted in a developing country using light, electron microscope and animal inoculation.

[Application of chromosome painting to analysis of structural aberration in five cases].

Objective: This article reports that competitive hybridization using entire chromosome specific libraries as probe and human genomic DNA as the competitor allows intense and specific fluorescent staining of human chromosome in metaphase. This general approach is called "chromosome painting".

Methods: The probes comprising chromosomes 2, 5, 6, 7, 13, 14, X specific libraries were used to analyse five cases which had been suspected of subtle translocation and deletion in karyotype analysis by G-banding of metaphase cells.

Amino acids excess increase SCEs in human lymphocytes.

The effects of 21 amino acids on sister-chromatid exchanges (SCEs) in human lymphocytes were investigated. Addition of each of the 21 amino acids, originally in a composition of the culture medium RPMI 1640, at three different concentrations (10 micrograms/ml, 50 micrograms/ml and 100 micrograms/ml) caused a dose-independent increase in SCE frequency. The results indicated that amino acids, although indispensable for cells in vivo and in vitro, may be SCE-inducing agents.

Effects of dNTPs on the sister-chromatid exchange (SCE) frequency in human lymphocytes.

The effects of deoxynucloside triphosphates (dNTPs) on the frequency of sister-chromatid exchange (SCE) was studied in human peripheral lymphocytes. Treatment with each dCTP and dTTP did not change the SCE frequency; however, dGTP caused a significant dose-dependent increase in SCE frequency, whereas dATP caused a significantly decreased in SCE frequency. The SCE-increasing effect of the treatment with dGTP could be totally reversed by treatment with equal concentrations of dATP or dCTP.

Effects of thiofedrine on platelet aggregation, thromboxane B2 and 6-keto-PGF1 alpha in rats.

Thiofedrine inhibited rat platelet aggregation and intraplatelet thromboxane B2 (TxB2) generation induced by arachidonic acid. The IC50 values were 0.18 and 0.

[A study of subtotal gastrectomy under the acupuncture combined with epidural anesthesia of small dosage].

Forty-eight cases with benign gastric diseases were operated using acupuncture at Zusanli (ST 36) and Neiguan (PC 6) combined with epidural anesthesia of small dosage (group A, 39 cases), and simple epidural anesthesia of small dosage (group B, 9 cases). The results show that the effect in the group A is better than that in the group B. Namely, the analgesia is stronger, the tractive reaction of internal organs is lighter, the abdominal muscles are more relaxed, the dosage of anesthetic is much smaller and the physiological disturbance is less in the group A.

A new structure of small intensely fluorescent cells in superior mesenteric ganglion of human fetus.

Superior mesenteric ganglia of six fetuses (35-40 weeks old) were investigated by histochemical fluorescent method. In addition to solitary SIF cells and clusters of SIF cells reported previously, a new structure of SIF cells was found and named "SIF-cell nodule". The SIF-cell nodule was composed of a large number of SIF cells and was encapsulated by dense connective tissue.

Influence of thiofedrine on platelet aggregation, intraplatelet cyclic cAMP and malondialdehyde in rats.

Platelet aggregation and cyclic adenosine monophosphate (cAMP) production were studied by turbidimetry and competitive protein binding assay, respectively, in rats. Thiofedrine (Thi) significantly inhibited adenosine diphosphate (ADP)-induced and thrombin-induced platelet aggregation in vitro, with IC50 values of 0.56 and 0.

[Relation of effects of matrine on rat vasa deferens to activation of calcium channels].

Effective mechanisms of matrine (Mat) in contraction were observed in isolated rat vasa deferens. Mat caused a strong concentration-dependent contraction of vasa deferens, and this contraction was competitively inhibited by prazosin (Pra, 10 mumol.L-1) and nifedipine (Nif, 50 nmol.

[Effects of Cistanche deserticola Y.C. Ma on serum creatine kinase and ultrastructures of skeletal muscles in mice].

The results showed that after mice were administrated with Cistanche deserticola, the duration of swimming was prolonged, and the increase of serum creative kinase was inhibited after exercises. Moreover, in the skeletal muscle ultrastructures it was found that after loaded swimming the glycogen became rich, and hyperplasia and hypertrophy of mitochondria occurred without any injury to myofibril.

[Effect of total alkaloids of kudouzi (Sophora alopecuroides L.) on tolerance to anoxia in mice].

To several anoxia mice models, total alkaloids of Kudouzi (TASa) can prolong their tolerance time (ip < 1/10, 1/5LD50/kg, P < 0.05-0.01).