A Feature Selection Approach Guided an Early Prediction of Anthocyanin Accumulation Using Massive Untargeted Metabolomics Data in Mulberry.

Identifying the early predictive biomarkers or compounds represents a pivotal task for guiding a targeted agricultural practice. Despite the various available tools, it remains challenging to define the ideal compound combination and thereby elaborate an effective predictive model fitting that. Hence, we employed a stepwise feature selection approach followed by a maximum relevance and minimum redundancy (MRMR) on the untargeted metabolism in four mulberry genotypes at different fruit developmental stages (FDSs).

Effects of long-term straw retention on soil microorganisms under a rice-wheat cropping system.

The objective of this study was to investigate how straw-incorporating practices affect bacterial communities and carbon source utilization capacity under a rice-wheat rotational farming practice in central China. To clarify the effect of long-term straw incorporation in microbial abundance and carbon metabolism, a long-term field experiment was initiated in May 2005 (rice-planting season). Soil bacterial communities were revealed by high-throughput sequencing technology.

Response of soil microbial community to application of biochar in cotton soils with different continuous cropping years.

The bacterial community in soils of cotton that have continuously been cropped for 2 years, 6 years, 11 years and 14 years and treated with biochar (B0, 0 t·ha; B1, 12.5 t·ha; and B2, 20 t·ha) was investigated using next-generation sequencing. Of the 45 bacterial genera (relative abundance ratio of genera greater than 0.

Response of Bacteria Community to Long-Term Inorganic Nitrogen Application in Mulberry Field Soil.

The bacterial community and diversity in mulberry field soils with different application ages of inorganic nitrogen fertilizer (4Y, 4-year-old; 17Y, 17-year-old; 32Y, 32-year- old) were investigated using next-generation sequencing. The results demonstrated that the application ages of nitrogen fertilizer significantly altered soil bacterial community and diversity. Soil bacterial Shannon diversity index and Chao 1 index decreased with the consecutive application of nitrogen fertilizer, and the 4Y soil exhibited the highest bacterial relative abundance and diversity.

Soil fungal community comparison of different mulberry genotypes and the relationship with mulberry fruit sclerotiniosis.

Mulberry fruit sclerotiniosis is a common soil-borne disease. We hypothesize that there is a relationship between the incidence of mulberry fruit sclerotiniosis and the soil fungal community. Therefore four mulberry genotypes with different resistance to sclerotiniosis were selected to study their soil fungal community under the same condition.

Histone Modification Is Involved in Okadaic Acid (OA) Induced DNA Damage Response and G2-M Transition Arrest in Maize.

Histone modifications are involved in regulation of chromatin structure. To investigate the relationship between chromatin modification and cell cycle regulation during plant cell proliferation, Okadaic acid (OA), a specific inhibitor of serine/threonine protein phosphatase, was applied in this study. The results showed that OA caused the cell cycle arrest at preprophase, leading to seedling growth inhibition.

Histone acetylation and reactive oxygen species are involved in the preprophase arrest induced by sodium butyrate in maize roots.

Histone acetylation plays a critical role in controlling chromatin structure, and reactive oxygen species (ROS) are involved in cell cycle progression. To study the relationship between histone acetylation and cell cycle progression in plants, sodium butyrate (NaB), a histone deacetylase (HDAC) inhibitor that can cause a significant increase in histone acetylation in both mammal and plant genomes, was applied to treat maize seedlings. The results showed that NaB had significant inhibition effects on different root zones at the tissue level and caused cell cycle arrest at preprophase in the root meristem zones.