"We Don't Get Drugs Targeted for Us:" Applying the Integrated Behavioral Model to Understand Why Black Women Chose to Participate in a Breast Cancer Clinical Trial.

Guided by the integrated behavioral model, the authors interviewed 14 Black breast cancer survivors ( = 14) who had participated in a breast cancer clinical trial. This study aimed to better understand what may motivate Black women to engage in medical research and decide to participate in medical research. Findings revealed that Black women's altruistic desires to serve others and their communities are greatly influenced by the need to leave a "legacy" of better treatment for other Black women.

Effect of novel benzoylphenylurea derivatives on DNA polymerase alpha activity using the synthesome-based in vitro model system.

Six benzoylphenylurea (BPU) derivatives have been synthesized in Japan and extensively evaluated by the U.S. National Cancer Institute.

The DNA synthesome: its potential as a novel in vitro model system for studying S-phase specific anticancer agents.

A considerable effort is being made to discover more effective anticancer agents and to develop rapid, accurate, and reliable methods for evaluating the usefulness of the new compounds and analyzing their mechanisms of action. In this regard, our laboratory was the first to report the isolation, characterization, and extensive purification of an intact, stable, and fully functional mammalian cell multiprotein complex, which we designated the DNA synthesome. The DNA synthesome has been isolated from a wide variety of mammalian cells as well as from tissues and has been shown to be fully competent to support SV40 origin-specific and large T antigen-dependent DNA replication in vitro.

Human cell DNA replication is mediated by a discrete multiprotein complex.

A discrete high molecular weight multiprotein complex containing DNA polymerase alpha has been identified by a native Western blotting technique. An enrichment of this complex was seen at each step in its purification. Further purification of this complex by ion-exchange chromatography indicates that the peak of DNA polymerase alpha activity co-purifies with the peak of in vitro SV40 DNA replication activity eluting from the column.

Isolation and characterization of primitive hematopoietic cells based on their position in the cell cycle.

Hematopoietic progenitor and stem cells are believed to lie dormant within the adult bone marrow microenvironment in a state characterized by both mitotic and metabolic quiescence. This state of cell-cycle quiescence has been the focus or target of many studies aimed at identifying cells with such mitotic properties for their eventual isolation and characterization. On the other hand, knowledge of the type, frequency, and primitive status of dividing cells in patients with malignant hematopoietic diseases is very important for the hematologist designing therapies aimed at targeting cycling cancer cells with cell-cycle-specific chemotherapuetic drugs that can spare noncycling normal hematopoietic stem cells (HSC).

14-3-3zeta interacts with the alpha-chain of human interleukin 9 receptor.

Interleukin 9 (IL-9) exerts its pleiotropic effects through the IL-9 receptor (IL-9R) complex, which consists of the IL-9R alpha-chain, which determines the cytokine specificity, and the IL-2 receptor gamma-chain. In the present study we used a modified yeast two-hybrid system to isolate cDNA species encoding proteins that interacted with the intracellular domain of the human IL-9R alpha-chain (hIL-9Ralpha). We have identified 14-3-3zeta as an hIL-9Ralpha-interacting protein.

Tip60 interacts with human interleukin-9 receptor alpha-chain.

Interleukin-9 (IL-9) exerts its pleiotropic effects through the IL-9 receptor (IL-9R) complex that consists of the ligand specific IL-9R alpha-chain, and the IL-2R gamma-chain. In this study, we used a modified yeast two-hybrid system to isolate cDNAs encoding proteins that interact with the intracellular domain of the human IL-9R alpha-chain (hIL-9Ralpha). We have identified Tip60, an HIV-1 Tat transcription cofactor, as an hIL-9Ralpha interacting protein.