Polycyclic aromatic hydrocarbon sequestration by intertidal phototrophic biofilms cultivated in hydrophobic and hydrophilic biofilm-promoting culture vessels.

Phototrophic biofilms collected from intertidal sediments of the world's largest tidal mangrove forest were cultured in two sets of a biofilm-promoting culture vessel having hydrophilic glass surface and hydrophobic polymethyl methacrylate surface wherein 16 priority polycyclic aromatic hydrocarbons (PAHs) were spiked. Biofilms from three locations of the forest were most active in sequestering 98-100% of the spiked pollutants. PAH challenge did not alter the biofilm phototrophic community composition; rather biofilm biomass production and synthesis of photosynthetic pigments and extracellular polymeric substances (EPS) were enhanced.

Role of intertidal microbial communities in carbon dioxide sequestration and pollutant removal: A review.

Intertidal microbial communities occur as biofilms or microphytobenthos (MPB) which are sediment-attached assemblages of bacteria, protozoa, fungi, algae, diatoms embedded in extracellular polymeric substances. Despite their global occurrence, they have not been reviewed in light of their structural and functional characteristics. This paper reviews the importance of such microbial communities and their importance in carbon dioxide sequestration as well as pollutant bioremediation.

Assessment of polycyclic aromatic hydrocarbon contamination in the Sundarbans, the world's largest tidal mangrove forest and indigenous microbial mixed biofilm-based removal of the contaminants.

The distribution of polycyclic aromatic hydrocarbons (PAHs) in the surface water and sediments in five regions of the Indian Sundarbans was assessed. The capability of microbial biofilm communities to sequester PAHs in a biofilm-promoting vessel was evaluated. The total PAH concentration of water and sediments ranged from undetectable to 125 ng ml and 4880 to 2 × 10 ng g dry weight respectively.

U32 collagenase from Pseudoalteromonas agarivorans NW4327: Activity, structure, substrate interactions and molecular dynamics simulations.

A protease of the primary pathogen (Pseudoalteromonas agarivorans NW4327) of the disease affecting the Great Barrier Reef sponge Rhopaloeides odorabile was purified. Zymography demonstrated calcium-dependent collagenase and gelatinase activity of the purified protein. This metalloprotease was identified by matrix assisted laser desorption ionization time-of-flight mass spectrophotometry as a 52,509 Da U32 collagenase.

A new collagenase enzyme of the marine sponge pathogen Pseudoalteromonas agarivorans NW4327 is uniquely linked with a TonB dependent receptor.

The primary pathogen of the Great Barrier Reef sponge Rhopaloeides odorabile, recently identified as a novel strain (NW4327) of Pseudoalteromonas agarivorans, produced collagenase which degraded R. odorabile skeletal fibers. We now report the collagenase of P.

Production enhancement and characterization of the polyhydroxyalkanoate produced by Natrinema ajinwuensis (as synonym) ≡ Natrinema altunense strain RM-G10.

Application of halophiles can decrease the cost of polyhydroxyalkanoate (PHA) production or bioplastic which are an alternative to the petroleum-derived plastic. Extremely halophilic archaeon, Natrinema ajinwuensis RM-G10 accumulated 61.02±0.