Influence of HLA-B Leader (-21M/T) Dimorphism With Bw4/Bw6 Epitopes on Graft Versus Host Disease After Allogenic Haematopoietic Stem Cell Transplantation in North Indians.

High degree of variability in human leukocyte antigens (HLAs) system restricts availability of histocompatible HLA-matched-related donors, thus increasing reliance on worldwide bone marrow registries network. Nevertheless, due to limited coverage/accessibility/affordability of some ethnicities in these registries, haploidentical haematopoietic stem cell transplantation (HSCT) emerged as an alternative option, though with allorecognition-mediated graft versus host disease (GvHD) (>40% cases). A dimorphism [-21 methionine (M) or threonine (T)] in HLA-B leader peptide (exon 1) which differentially influences its HLA-E binding, plausibly regulates natural killer cell functionality, affecting GvHD vulnerability and clinically in practice for donor selection.

Coumarin Analogues as Promising Anti-Obesity Agents: In Silico Design, Synthesis, and In Vitro Pancreatic Lipase Inhibitory Activity.

A set of coumarin-3-carboxamide analogues were designed, synthesized, and evaluated for their ability to impede pancreatic lipase (PL) activity. Out of all the analogues, 5dh and 5de demonstrated promising inhibitory activity against PL, as indicated by their respective IC values of 9.20 and 11.

Characterisation of the Novel HLA-DQB1*06:469 Allele by Next-Generation Sequencing.

The novel HLA-DQB1*06:469 allele differs from HLA-DQB1*06:01:01:01 by one nucleotide substitution in codon 187 in exon 3.

An international survey of patient blood management practices.

Background: The Association for the Advancement of Blood and Biotherapies (AABB) conducted a global survey of patient blood management (PBM) practices. It determined changes in PBM practices since the last survey.

Study Design And Methods: A working group of AABB's PBM Subsection and AABB staff designed the survey using the Qualtrics™ platform.

Point-of-care nucleic acid testing - a step forward in controlling the HIV epidemic: A review.

Introduction: The HIV/AIDS epidemic, with 85.6 million infections and 40.4 million AIDS-related deaths globally, remains a critical public health challenge.

Bi-S Bond Mediated Direct Z-Scheme BiOCl/Cu2SnS3 Heterostructure for Efficient Photocatalytic Hydrogen Generation.

The advancement of photocatalytic technology for solar-driven hydrogen (H2) production remains hindered by several challenges in developing efficient photocatalysts. A key issue is the rapid recombination of charge carriers, which significantly limits the light-harvesting ability of materials like BiOCl and Cu2SnS3 quantum dots (CTS QDs), despite the faster charge mobility and quantum confinement effect, respectively. Herein, a BiOCl/CTS (BCTS) heterostructure was synthesized by loading CTS QDs onto BiOCl 2D nanosheets (NSs), that demonstrated excellent photocatalytic activity under visible light irradiation.

Clinico-Pathological Significance of Tumor Infiltrating Immune Cells in Oral Squamous Cell Carcinoma-Hope or Hype?

Background: To correlate between immunohistochemical expression of tumor-infiltrating lymphocytes (TILs), tumor-associated macrophages (TAMs), and natural killer (NK) cells with the AJCC 8th edition TNM staging system and other disease-modifying clinico-pathological variables.

Methods: The representative histology sections of tumor invasive margin (IM) and tumor core (TC) were selected according to the International Immuno-Oncology Biomarker Working Group and were subjected to immunohistochemistry with antibodies for TILs (CD3, CD8, FOXP3), NK Cells (CD57), TAMs (CD68, CD163) and pan-leukocyte marker (CD45). Histo-immuno-density-intensity (HIDI) scoring was calculated as a product of the proportion and intensity of staining.

Drug-assisted White Light Generation via Self-assembly.

White-light generation using small organic molecules has gained significant attention from researchers working on the interface of supramolecular chemistry and organic materials. Self-assembled multi-chromophoric materials utilizing a drug molecule and microenvironment-sensitive intramolecular charge transfer dye as an emitter offer the possibility of tunable emission. In this investigation, we focused on white light generation via the combination of a polarity-sensitive red-emitting styryl chromone (SC) and a blue-emitting anticancer and psychotherapeutic drug Norharmane (NHM) in a self-assembled micellar system.

Noninvasive in vivo imaging of macrophages: understanding tumor microenvironments and delivery of therapeutics.

Macrophages are pivotal in the body's defense and response to inflammation. They are present in significant numbers and are widely implicated in various diseases, including cancer. While molecular and histological techniques have advanced our understanding of macrophage biology, their precise function within the cancerous microenvironments remains underexplored.

Fluoride Exposure Modulates Skeletal Development and Mineralization in Zebrafish Larvae.

The presence of high levels of fluoride (F) in groundwater is a major issue worldwide. Although F is essential for healthy teeth and bones, excessive exposure can cause fluorosis or F toxicity. This condition primarily affects the hard tissues due to their high F retention capacity.

Lifesaving surgical approaches for severe penetrating knife injury to the neck.

Background: Penetrating neck injuries are rare and require urgent surgical intervention to prevent life-threatening complications. This report highlights a unique case involving complex surgical repair of tracheal, esophageal, and vascular injuries following a homicidal assault, emphasizing the challenges and techniques used in managing such severe trauma.

Case Presentation: A 45-year-old female presented with a severe penetrating neck injury after an alleged homicidal assault with a knife.

Population structure, distribution pattern and habitat utilization of Yellow-wattled Lapwing, Vanellus malabaricus (Boddaert, 1783), in Northern India.

This field study investigated the population structure, distribution pattern and habitat utilization of Yellow-wattled Lapwings. The line transect method was used to estimate the density of Yellow-wattled Lapwings. An average of 394 individuals including 77 chicks of Yellow-wattled Lapwings have been registered with an average density of 38 birds per km.

Eco-Friendly Fabrication of FeS QD-Chitosan Biopolymer Composites: Green Synthetic Approach.

In this paper, we offer a unique green synthetic approach for producing iron sulfide quantum dots (FeS QD)-chitosan composites using gel chemistry. The technique uses the environmental features of chitosan, a biocompatible and biodegradable polysaccharide, and the excellent electrical properties of FeS QDs. By sustainable chemistry principles, the synthesis process is carried out under gentle settings, using aqueous solutions and avoiding hazardous solvents and strong chemicals.

Organic manure and fertilizer N management strategies improve soil health at different growth stages of pearl millet under pearl millet-wheat sequence.

Background: Incorporating organic manure improves soil properties and crop productivity. A long-term study started in October 1967 examined the effects of farmyard manure and nitrogen fertilization on the soil at key growth stages of pearl millet in a pearl millet-wheat cropping system over its 51st cycle.

Results: Applying 15 Mg of farmyard manure (FYM) per hectare in both growing seasons significantly boosted soil organic carbon (SOC), dissolved organic carbon (DOC), and key nutrients compared to one-season application.

Exploring the role of the JEEViKA swasthya mitra helpdesk in improving healthcare access: a qualitative study in tertiary healthcare facilities in Bihar, India.

Background: Rural populations in Bihar, India, face significant healthcare access challenges due to geographical, infrastructural, and financial barriers. The Swasthya Mitra program, initiated by the Bihar Rural Livelihood Promotion Society in collaboration with local and international partners, aims to mitigate these challenges by employing trained community members to navigate patients through the healthcare system.

Methods: This qualitative study employed in-depth interview and thematic analysis to evaluate the Swasthya Mitra program in the Bhagalpur and Jamui districts in Bihar, India.

Diagnostic and therapeutic strategies in combating implanted medical device-associated bacterial biofilm infections.

Bacterial biofilms exhibit remarkable resistance against conventional antibiotics and are capable of evading the humoral immune response. They account for nearly 80% of chronic infections in humans. Development of bacterial biofilms on medical implants results in their malfunctioning and subsequently leads to high mortality rates worldwide.

Nutraceutical Impact of Pumpkin Seed Oil on Expression Levels of EZH-2 and KRT-14 Genes against DSS-induced Inflammatory Bowel Disease in the Rat Model.

Inflammatory bowel disease is a collection of intestinal disorders that cause inflammation in the digestive tract. Prolonged inflammation in the gastrointestinal tract is a major risk factor for colorectal cancer. The objective of this study was to fucus on gene expression levels of (KRT-14; associated with epithelial cell integrity) and enhancer of zeste homolog-1 (EZH-2; involved in cellular proliferation) in a IBD rat model in order to rule out impact of nutraceuticals (pumpkin seed oil; PSO) as a complementary approach to conventional treatments of IBD.