DNA Dendron Tagging as a Universal Way to Deliver Proteins to Cells.

The use of proteins as intracellular probes and therapeutic tools is often limited by poor intracellular delivery. One approach to enabling intracellular protein delivery is to transform proteins into spherical nucleic acid (proSNA) nanoconstructs, with surfaces chemically modified with a dense shell of radially oriented DNA that can engage with cell-surface receptors that facilitate endocytosis. However, proteins often have a limited number of available reactive surface residues for DNA conjugation such that the extent of DNA loading and cellular uptake is restricted.

Enhancing quality of life in individuals with cerebral palsy: a systematic review and meta-analysis of physiotherapy interventions.

Purpose: This systematic review examined studies that addressed physiotherapy intervention approaches to the Quality of Life (QoL) of people with Cerebral Palsy (CP).

Materials And Methods: We conducted a comprehensive search strategy in five databases (PEDro, PubMed, Web of Science, Scopus, and Google Scholar) until 12 February 2024. We assessed the included studies' methodological quality and statistical description using the PEDro scale.

Intranasal Calcitriol Accelerates Improvement of Sinonasal Inflammation and Olfactory Impairment in Mice After Cessation of Chronic Cigarette-Smoke Exposure.

Rationale: Smoking has been shown to be associated with circulating deficiencies in 25(OH)D3 and reduced sinonasal tissue levels of the active form of vitamin D, 1,25(OH)2D3. Given vitamin D's ability to reduce inflammation, we sought to examine if intranasal (IN) delivery of calcitriol [clinical analog of 1,25(OH)2D3] could reduce inflammation and improve disease severity in a murine model of chronic cigarette smoke-induced sinonasal inflammation (CS-SI).

Methods: Mice were exposed to CS 5 h/day, 5 days/week for 9 months, and then began IN calcitriol three times per week for 4 weeks.

Geographic patterns and climatic drivers of the mean genus age of liverworts in North America.

Phylogenetic niche conservatism posits that species tend to retain ancestral ecological traits and distributions, which has been broadly tested for lineages originating in tropical climates but has been rarely tested for lineages that originated and diversified in temperate climates. Liverworts are thought to originate in temperate climates. Mean lineage age reflects evolutionary history of biological communities.

Honoring Hartmut Karl Lichtenthaler, innovative pioneer of photosynthesis, on his 90 birthday.

We honor Professor Hartmut Karl Lichtenthaler, a versatile pioneer of photosynthesis research, plant physiology, isoprenoid biochemistry, and stress physiology of plants, for his groundbreaking and creative contributions to plant science. His innovative research on the chemical composition, ultrastructure, and function of chloroplasts and his detection of the major methylerythritol 4-phosphate (MEP) isoprenoid biosynthetic pathway in plants is key to our current understanding of the physiology and biochemistry of photosynthesis systems. His ingenious use of the powerful laser-induced chlorophyll fluorescence imaging has helped us better understand the stress response processes in plant leaves.

Computerized cognitive testing to capture cognitive decline in Alzheimer's disease: Longitudinal findings from the ARMADA study.

Introduction: Timely detection and tracking of Alzheimer's disease (AD) -related cognitive decline has become a public health priority. We investigated whether the NIH Toolbox for Assessment of Neurological and Behavioral Function-Cognition Battery (NIHTB-CB) detects AD-related cognitive decline.

Methods:  = 171 participants (age 76.

Prevalence of hepatitis C virus co-infection in adults living with HIV in Mexico: a cross-sectional, seroprevalence study in a nationally representative sample.

Background: The proportion of people living with HIV (PLWHIV) co-infected with HCV in Mexico was unknown. Our aim was to estimate the seroprevalence of HCV among adults with HIV in Mexico.

Methods: Using a complex-survey design, we collected blood samples and applied structured questionnaires between May 2nd, 2019 and February 17th, 2020 in a nationally, representative sample of adults receiving care for HIV-infection in 24 randomly selected HIV-care centres in 8 socio-demographically regions in Mexico.

Use of pattern recognition in photoacoustic imaging to identify neuronal ensembles in the prefrontal cortex of rats undergoing conditioned fear learning.

Pattern recognition analysis in brain research has improved understanding of sensory processing and led to the identification of default brain networks in neuroimaging studies. The current study uses pattern recognition analysis to extend our previous findings showing conditioned fear learning and novelty-exposure (i.e.

Synchronous primary aneurysmal bone cysts: A rare case report and literature review.

Aneurysmal bone cysts (ABCs) are rare benign osteolytic tumors which occur most frequently within the first two decades of life and can lead to pathological fractures. They have characteristic imaging and histologic features and have historically been classified into two types: primary ABCs, which are true neoplasms linked to specific oncogenes, and secondary ABCs, which are preceded by other bone lesions. The occurrence of multiple concurrent primary ABCs is exceedingly rare; with only a few cases reported in the published literature.

The Roots of Health Inequality and the Value of Intrafamily Expertise.

In the context of Sweden, we show that having a doctor in the family raises preventive health investments throughout the life cycle, improves physical health, and prolongs life. Two quasi-experimental research designs-medical school admission lotteries and variation in the timing of medical degrees-support a causal interpretation of these effects. A hypothetical policy that would bring the same health behavior changes and benefits to all Swedes would close 18 percent of the mortality-income gradient.

A Comprehensive Assessment of the Marginal Abatement Costs of CO of Co-Optima Multi-Mode Vehicles.

The Co-Optimization of Fuels and Engines (Co-Optima) is a research and development consortia funded by the U.S. Department of Energy, which has engaged partners from national laboratories, universities, and industry to conduct multidisciplinary research at the intersection of biofuels and combustion sciences.

Recommendations for Specialty Palliative APRN Graduate Education.

Background: Advanced practice registered nurses (APRNs) are essential to care for the growing number of individuals with advanced illness given the shortage of palliative care clinicians.

Problem: Graduate education for specialty practice palliative APRNs lacks consistency in structure, content, and standardization of specialty palliative APRN education.

Approach: A workgroup of expert palliative APRNs and graduate faculty conducted focused discussions and a literature review to develop consensus recommendations based on national palliative APRN competencies and aligned with the 8 core concepts of the AACN Essentials.

Patient-reported outcomes in genital gender-affirming surgery.

Purpose Of Review: We reviewed the latest articles in patient-reported outcomes as it relates to genital gender-affirming surgery (GGAS) and provide a narrative summary of each article.

Recent Findings: The current landscape of patient-reported outcomes measures (PROMs) within GGAS largely consists of various ad hoc questionnaires. Within the last two years, one new PROM, validated within the transgender and gender-diverse (TGD) community, have been described.

Mapping snoRNA Targets Transcriptome-Wide with snoKARR-seq.

Small nucleolar RNAs (snoRNAs) are noncoding RNAs primarily known for guiding chemical modifications of RNA, but their broader cellular roles and contributions to human diseases remain elusive. This In Focus article introduces the development of snoRNA-enriched kethoxal-assisted RNA-RNA sequencing (snoKARR-seq), a transcriptome-wide approach to uncover snoRNA targets with enhanced sensitivity and specificity. This method revealed an unexpected role for snoRNAs in protein translocation and secretion, expanding our understanding of their noncanonical functions.

Community Implementation and Evaluation of the Chicago Parent Program.

The purpose of this study was to describe and evaluate the implementation of the Chicago Parent Program (CPP) in a community setting. Included are details of the co-designed implementation by academic and community partners, and parenting and child behavior outcomes. Two hundred eighty caregivers of children aged 2-5 years participated in the CPP in seven community sites.

Effect of hearing experience on preschool-aged children's eye gaze to a talker during spoken language processing.

Speechreading-gathering speech information from talkers' faces-supports speech perception when speech acoustics are degraded. Benefitting from speechreading, however, requires listeners to visually fixate talkers during face-to-face interactions. The purpose of this study is to test the hypothesis that preschool-aged children allocate their eye gaze to a talker when speech acoustics are degraded.

Co-targeting of metabolism using dietary and pharmacologic approaches reduces breast cancer metastatic burden.

Patients with metastatic breast cancer face reduced quality of life and increased mortality rates, necessitating more effective anti-cancer strategies. Building on previous research that identified metastatic-niche-specific metabolic vulnerabilities, we investigated how a ketogenic diet enhances estrogen receptor (ER)-positive liver metastatic breast cancer's response to Fulvestrant (Fulv) treatment. Using in vitro cell lines and in vivo xenograft metastasis mouse models, we examined the molecular mechanisms of combining ER targeting with a ketogenic diet.

Intervalence plasmons in boron-doped diamond.

Doped semiconductors can exhibit metallic-like properties ranging from superconductivity to tunable localized surface plasmon resonances. Diamond is a wide-bandgap semiconductor that is rendered electronically active by incorporating a hole dopant, boron. While the effects of boron doping on the electronic band structure of diamond are well-studied, any link between charge carriers and plasmons has never been shown.

Italian Vocal Fatigue Index: Cross-Cultural Adaptation and Validation.

Background: Vocal fatigue involves self-perceived vocal symptoms and reduced physiological capacity. This study aimed to adapt and validate the Vocal Fatigue Index (VFI), a tool originally designed to distinguish between patients with vocal fatigue and vocally healthy individuals, for Italian speakers.

Method: A four-step translation and validation process was employed.

eIF4F-mediated Dysregulation of mRNA Translation in Cancer.

Messenger RNA (mRNA) translational control plays a pivotal role in regulating cellular proteostasis under physiological and pathological conditions. Dysregulated mRNA translation is pervasive in cancer, in which protein synthesis is elevated to support accelerated cell growth and proliferation. Consequently, targeting the mRNA translation machinery has emerged as a therapeutic strategy to treat cancer.

Type 1 Diabetes Mellitus and Thromboembolism in Pregnancy.

Objective The impact of type 1 DM (T1DM) on thromboembolism in pregnancy is uncertain. We hypothesized that T1DM is associated with higher rates of thrombotic events during pregnancy and the postpartum period. Study Design This is a retrospective cohort study utilizing the National Inpatient Sample database from HCUP/AHRQ for 2017-2019.

Responsiveness to cold snaps by turtle embryos depends on exposure timing and duration.

Characterizing how organisms respond to transient temperatures may further our understanding of their susceptibility to climate change. Past studies in the freshwater turtle, , have demonstrated that the timing and duration of heat waves can have major implications for the response of genes involved in gonadal development and the production of female hatchlings. Yet, no study has considered how the response of these genes to transient cold snap exposure may affect gonadal development and the production of males.

Parallel genetic adaptation amid a background of changing effective population sizes in divergent yellow perch () populations.

Aquatic ecosystems are highly dynamic environments vulnerable to natural and anthropogenic disturbances. High-economic-value fisheries are one of many ecosystem services affected by these disturbances, and it is critical to accurately characterize the genetic diversity and effective population sizes of valuable fish stocks through time. We used genome-wide data to reconstruct the demographic histories of economically important yellow perch () populations.

Decoding speech intent from non-frontal cortical areas.

Brain-machine interfaces (BMIs) have advanced greatly in decoding speech signals originating from the speech motor cortices. Primarily, these BMIs target individuals with intact speech motor cortices but who are paralyzed by disrupted connections between frontal cortices and their articulators due to brainstem stroke or motor neuron diseases such as amyotrophic lateral sclerosis. A few studies have shown some information outside the speech motor cortices, such as in parietal and temporal lobes, that also may be useful for BMIs.