3 results match your criteria: "INRA Centre de Recherches Agronomiques de Rennes[Affiliation]"

Article Synopsis
  • PCR techniques revealed Mycosphaerella graminicola infections in wheat crops three weeks before symptoms appeared, allowing differentiation between curative and preventative treatments.
  • Azoxystrobin, when applied curatively after infection onset, showed intermediate effectiveness compared to chlorothalonil (low effect) and epoxiconazole (higher effectiveness), with no fungicide able to completely prevent leaf infections.
  • Both treatment types increased the time between initial infection detection and visible leaf damage, but azoxystrobin only slightly slowed the progression of necrosis compared to epoxiconazole, indicating its primary role in delaying visible damage rather than preventing infection.
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Green fluorescent protein (GFP)-expressing transformants were used to investigate the effects of strobilurin fungicide azoxystrobin on Mycosphaerella graminicola infection. Azoxystrobin treatments (125 or 250 g AI ha-1) were applied at various stages of the infection process under controlled conditions. GFP transformants showed conserved in vitro sensitivity to azoxystrobin and pathogenicity.

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A Mycosphaerella graminicola strain transformed with the green fluorescent protein (GFP) downstream of either a carbon source-repressed promoter or a constitutive promoter was used to investigate in situ carbohydrate uptake during penetration of the fungus in wheat leaves. The promoter region of the acu-3 gene from Neurospora crassa encoding isocitrate lyase was used as a carbon source-repressed promoter. The promoter region of the Aspergillus nidulans gpdA gene encoding glyceraldehyde-3-phosphate dehydrogenase was used as a constitutive promoter.

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