Evaluating the efficacy of Lactobacillus acidophilus derived postbiotics on growth metrics, Health, and Gut Integrity in broiler chickens.

Continuous use of antibiotics in poultry feed as growth promoters poses a grave threat to humanity through the emergence of antibiotic resistance, necessitating the exploration of novel and sustainable alternatives. The present study was carried out to evaluate the performance of postbiotics derived from Lactobacillus acidophilus in broiler birds. The postbiotics were harvested by culturing probiotic bacteria from the stock cultures at the required temperature and duration under laboratory conditions and supplemented to broilers via feed.

Transcriptome profiling of Canine Parvovirus 2 Nonstructural gene 1(CPV2.NS1) transfected 4T1 mice mammary tumor cells to elucidate its oncolytic effects.

Oncolytic viral gene therapy is a directed approach to target cancer cells without affecting healthy cells of the body. Canine parvovirus (CPV2) is an oncolytic virus that precisely targets and destroys neoplastic cells by causing DNA damage, mitochondrial damage, and apoptosis. Non-structural gene 1 (NS1) of CPV, concerned with viral DNA replication is a key mediator of cytotoxicity of CPV and can specifically cause tumor cell lysis.

Dietary concoction of formic acid and thymol and its effects on zoo-technical performance, immunity, jejunal architecture and gut health in Turkey.

With increasing demand for improved protein-rich food, diverse poultry birds like turkey are gaining popularity in India. However, with the rising concerns of antibiotic residues and drug resistance, safe alternatives like formic acid (FA) and thymol (TH) have attracted the attention of researchers as effective replacer of antibiotic growth promoters (AGP). This experiment investigated the effects of combinations of FA and TH on growth performance, immunity, gut microflora and jejunal microstructures in turkey.

The cage-free egg sector: perspectives of Indian poultry producers.

India is ranked as the 2nd largest egg producer in the world. Despite the prevalence of backyard poultry (free range), a majority of the commercial egg-laying hens in the country are still housed in battery cages. There is a global shift toward cage-free eggs, due to regulations and increased demand from conscious consumers and food corporations.

Adequate Levels of Zinc and Copper on Turkey Poult Performance, Immunological Response, and Skeletal Health.

In this study, the impact of varying dietary zinc (Zn) and copper (Cu) levels on the growth, immunological response, and skeletal health of developing turkey poults was investigated. For 0-4 and 5-8 weeks of age, respectively, three Zn levels of 50, 70, and 90 mg/kg and 45, 65, and 85 mg/kg were employed. Three Cu levels, namely 8, 12, and 16 mg/kg for 0-8 weeks of age, were also utilized.

Evaluation of in-feed supplementation of formic acid and thymol as non-antibiotic growth promoters and assessing their effect on antimicrobial resistant E.coli isolated in Turkey.

With the rampant usage of antibiotics as growth promoters (AGPs) in poultry sector, there has been alarming concerns of antimicrobial resistant microbes such as Escherichia coli. Diversification of poultry farming due to consumer demand for safer products with higher protein content, turkey production is gaining popularity. Feed additives such as formic acid (FA) and thymol (TH) are effectively replacing AGPs due to their antimicrobial action.

Transportation stress: Impact on behaviour and welfare in meat-type chickens under indian scenario.

In order to find standard transport time and its effect on the welfare, 480 marketable commercial broiler chickens (CARIBRO VISHAL; avg. 2.0 kg body weight) were transported for 2, 4, 8h and without transport in crates in three replicates in a completely randomized design.

Development of superoxide dismutase based visual and spectrophotometric method for rapid differentiation of fresh and frozen-thawed buffalo meat.

Study aimed to develop biomarker-based assay for rapid detection of fresh and frozen-thawed buffalo meat in the supply chain. The method is based on development of a solvent system and identification of suitable substrate and developer for screening of biomarkers. For the confirmation column chromatography, gel electrophoresis and Western Blotting were carried out.

Production performance, haematological parameters, serum biochemistry, and expression of HSP-70 in broiler chickens fed dietary ascorbic acid during heat stress.

An experiment was carried out to assess the efficacy of supplemental ascorbic acid (AA) on broiler chicken production performance, blood haematological profile, biochemical profile, and carcass traits under heat stress conditions. A total of 192-day-old broiler chicks were divided into four groups, each with six replicates of eight each (4 × 6 × 8). Four corn-based dietary treatments were formulated: T (control diet), T (T + AA at 200 mg/kg), T (T + AA at 400 mg/kg), and T (T + AA at 600 mg/kg) for a period of 42 days.

Changes in mA RNA methylation of goat lung following PPRV infection.

Peste des petits ruminants (PPR) is an acute, highly contagious viral disease of goats and sheep, caused by the Peste des petits ruminants virus (PPRV). Earlier studies suggest the involvement of diverse regulatory mechanisms in PPRV infection. Methylation at N6 of Adenosine called mA is a type RNA modification that influences various physiological and pathological phenomena.

Impact of bioclimatic factors on physio-biochemical and molecular response of slow-growing poultry reared in tropics.

Most of the climatic studies projected on heat stress have considered heat extremes, but not the humidity. Hence, this work was carried out to evaluate thermotolerance, production performance, physio-biochemical and immunological response of slow-growing poultry towards various temperature-humidity levels in coastal climate. A total of 240 straight run CARI-Debendra birds were reared in three groups based on temperature-humidity indices (THI > 80, = 75-80 and < 75).

Effects of Various Copper Sources and Concentrations on Performance, Skeletal Growth, and Mineral Content of Excreta in Broiler Chickens.

The experiment was designed to study the effect of supplemental sources and concentrations of copper on the performance and development and mineralization of tibia bones in broiler chickens. A 42-day feeding experiment was conducted utilising three copper sources, including copper sulphate (CuS), copper chloride (CuCl), and copper propionate (CuP), each with four different concentrations, i.e.

Estimation of microsatellite-based autozygosity and its correlation with pedigree inbreeding coefficient in crossbred cattle.

In countries where farming is largely subsistence, no pedigree records of farm animals are maintained at farmers' herd and scientific mating plans are not observed which leads to the accumulation of inbreeding and loss of production potential. Microsatellites have been widely used as reliable molecular markers to measure inbreeding. We attempted to correlate autozygosity estimated from microsatellite data with the inbreeding coefficient () calculated from pedigree data in Vrindavani crossbred cattle developed in India.

Supplementary nucleosides improve the performance through enhanced enzyme activity, intestinal development and transporter genes in broiler chickens.

A biological experiment was carried out to evaluate dietary nucleoside supplementation on growth performance, digestive enzymes activities, immune response, and intestinal transporter genes expression in broiler chicken. A total of 720 newly hatched CARIBRO VISHAL broiler chicks were weighed and randomly divided into eight groups with nine replicates. The dietary treatments were as follows: Group I: diet without antibiotic supplement (control), group II: diet supplemented with antibiotic (positive control), groups III, IV and V: diet supplemented with combination of nucleosides at 0.

Effects of paraprobiotic as replacements for antibiotic on performance, immunity, gut health and carcass characteristics in broiler chickens.

This study sought to determine the effects of dietary paraprobiotic (PPB) on broiler chicken performance, immunity, gut health, and carcass traits. A total of 240 day-old CARIBRO Vishal commercial broiler chicks of identical body weight randomly divided into six treatment groups, each with five replicates and eight chicks in each replicate. Six dietary treatments were preapared: T = (control diet), T = T + 0.

nano-silver and nutrient supplementation improves immunity and resistance against Newcastle disease virus challenge in broiler chickens.

Silver nanoparticles (AgNPs) interact with the microbes and host immune system to protect against diseases. Fertile broiler eggs ( = 900) were allotted to six groups: un-injected control, sham (sterile water), AgNPs (50 μg), AgNPs+Amino acids (Methionine-10 mg + Arginine-25 mg), AgNPs+Vitamins (Vit B1-72μg + Vit B6-140μg), and AgNPs+Trace Elements (Zn-80 μg and Se-0.3 μg) and incubated for 18 days.

Exploration of allelic variants in short tandem repeats (STRs) flanking milk production QTLs and their association with milk production traits in Indian water buffaloes.

Short tandem repeats (STRs) are co-dominant, highly polymorphic marker loci, distributed throughout the genome and useful for identification and mapping of QTLs associated with variation in traits of economic significance. Allelic variants were identified in STRs located in close vicinity of cattle QTLs for milk production,, viz. BMS713, BM6404, BM4513, BM121, BM6105, TGLA245, BL1100, BMS1948, BMS711, BM1443, BM1706, BM6438, BM143, BM415, ETH131, ETH 2, and BM1329 in 109 water buffaloes of Murrah breed.

Seasonal variations on semen quality attributes in turkey and egg type chicken male breeders.

A biological experiment was carried out in twenty-four adult healthy breeder males each in turkey (Beltsville small white) and egg type chicken (White Leghorn Babcock) in order to assess the seasonal influence on semen production and quality. The birds were maintained in individual cages under uniform husbandry conditions throughout the year. The birds were fed with breeder ration and water ad libitum was offered with a constant photoperiod of 14 h/day.

Microarray analysis and PCR validation of genes associated with facultative parthenogenesis in Meleagris gallopavo (Turkey).

A cDNA microarray containing 43,661 differentially expressed genes was carried out on the blastoderm of fertilized and facultative parthenogenic turkey embryos at different hours of development. The total number of up-regulated (UR) and down-regulated (DR) genes at 0, 12, and 24 h of development were 725 and 1436, 942 and 599, and 589 and 1044, respectively. Common genes between 0 and 12 h, 12 and 24 h, and 0 and 24 h were 55, 67, and 110, respectively.

Performance enhancing, immunomodulatory, anti-hyperlipidaemic, and antimicrobial properties of bael (Aegle marmelos) leaf powder in broiler chicken.

This study investigated the effects of bael leaf powder (BLP) on growth performance, immunity, serum biochemistry, and caecal microbiology of broiler chickens. Following completely randomised design, a total of 288-day-old CARIBRO-Vishal broiler chickens were randomly divided into six treatment groups consisting of experimental diets formulated as follows - T1 (basal diet), T2 (basal diet + 250 mg bacitracin methylene disalicylate-BMD/kg diet), T3 (basal diet + 5 g BLP/kg diet) T4 (basal diet + 10 g BLP/kg diet), T5 (basal diet + 15 g BLP/kg diet), and T6 (basal diet + 20 g BLP/kg diet). Proximate analysis of green bael leaves revealed 65.

Effects of Spirulina (Arthrospira platensis) as a drinking water supplement during cyclical chronic heat stress on broiler chickens: Assessing algal composition, production, stress, health and immune-biochemical indices.

Spirulina, the blue green algae is considered to exhibit multifaceted benefits on both human health and animal production. Three hundred sixty day old unsexed broiler chicks of CARIBROVISHAL strain were assigned to five treatment groups each comprising nine replicates of 8 chicks. The experiment was carried out during the hot humid summer season (Mid-April to May) under deep litter rearing system with uniform managemental conditions.

Effect of dietary mannan oligosaccharides and fructo-oligosaccharides on physico-chemical indices, antioxidant and oxidative stability of broiler chicken meat.

The objective of this present study was to investigate the potentiality of prebiotics (mannan oligosaccharides-MOS and fructo-oligosaccharides-FOS) in replacement of antibiotic growth promoter and their relationship with physico-chemical indices, antioxidant and oxidative stability and carcass traits of broiler chickens meat. Accordingly, 240 day-old broiler chicks of uniform body weight divided in 6 treatment groups with 5 replicate each (5 × 6 = 30) having 8 birds in each replicate. Six corn based dietary treatments were formulated viz.

Hepatic transcriptome analysis reveals altered lipid metabolism and consequent health indices in chicken supplemented with dietary Bifidobacterium bifidum and mannan-oligosaccharides.

This study investigated the role of dietary prebiotic mannan-oligosaccharides (MOS), and probiotic Bifidobacterium bifidum (BFD) in lipid metabolism, deposition, and consequent health indices in broiler chicken. The supplementation of 0.2% MOS along with either 10 or 10 CFU BFD/g feed resulted in downregulation of Acetyl-CoA carboxylase, fatty acid synthase, sterolregulatory element binding protein-1, and apolipoprotein B100; and up-regulation of peroxisome proliferator activated receptor-α AMP-activated protein kinase α-1, and stearoyl CoA (∆9) desaturase-1 hepatic expression in broiler chicken.

Expression of nutrient transporter genes in response to dietary rice gluten meal and protease enzyme supplementation and the consequent effects on growth, nutrient digestibility, immunity and jejunum histomorphometry in chicken.

The objective of this study was to investigate the effects of feeding rice gluten meal (RGM) as an alternative protein source along with protease enzyme supplementation on growth performance, expression of nutrient transporter genes, nutrient digestibility, immune response and gut histomorphometry of broiler chicken. Proximate analysis of RGM revealed 923 g dry matter (DM), 500 g crude protein (CP), 69.2 g ether extract, 94.

Canine Parvovirus and Its Non-Structural Gene 1 as Oncolytic Agents: Mechanism of Action and Induction of Anti-Tumor Immune Response.

The exploration into the strategies for the prevention and treatment of cancer is far from complete. Apart from humans, cancer has gained considerable importance in animals because of increased awareness towards animal health and welfare. Current cancer treatment regimens are less specific towards tumor cells and end up harming normal healthy cells.