292 results match your criteria: "ICAR - National Institute for Plant Biotechnology[Affiliation]"

Stay-green (SG) and stem reserve mobilization (SRM) are two significant mutually exclusive traits, which contributes to grain-filling during drought and heat stress in wheat. The current research was conducted in a genome-wide association study (GWAS) panel consisting of 278 wheat genotypes of advanced breeding lines to find the markers linked with SG and SRM traits and also to screen the superior genotypes. SG and SRM traits, viz.

View Article and Find Full Text PDF

Small RNA sequencing analysis in two chickpea genotypes, JG 62 (Fusarium wilt-susceptible) and WR 315 (Fusarium wilt-resistant), under Fusarium wilt stress led to identification of 544 miRNAs which included 406 known and 138 novel miRNAs. A total of 115 miRNAs showed differential expression in both the genotypes across different combinations. A miRNA, Car-miR398 targeted copper chaperone for superoxide dismutase (CCS) that, in turn, regulated superoxide dismutase (SOD) activity during chickpea-Foc interaction.

View Article and Find Full Text PDF

The present study investigated the linkage between days to flowering (DTF) and growth habit (GH) in pigeonpea using QTL mapping, QTL-seq, and GWAS approaches. The linkage map developed here is the largest to date, spanning 1825.56 cM with 7987 SNP markers.

View Article and Find Full Text PDF

Most of the released high-yielding hybrids of have a low 1000-seed weight (TSW) with no increment in the percent oil content (OC), and, therefore, these hybrids have poor acceptance among the farmers in India. It is, thus, imperative to understand the genetic basis of these traits and deploy them in commercial hybrid breeding programs. The present study utilized a set of 15 diverse genotypes with TSW and OC ranging from 1.

View Article and Find Full Text PDF
Article Synopsis
  • Early seedling vigour (ESV) is crucial for rice cultivation, particularly in direct-seeded systems, and the aus varietal group shows high ESV.
  • A study of 181 aus accessions revealed significant genetic variation in ESV traits, and the vegetative vigour was closely linked to overall plant performance.
  • Genome-wide association studies identified 14 significant QTLs associated with ESV, including novel QTLs, and pinpointed candidate genes that may enhance seedling vigour and grain yield in breeding programs.
View Article and Find Full Text PDF

To meet the challenges of increasing food production demand globally, extracting insights regarding the persistent agriculture-related problems on a nationwide scale is the need of the hour. Policymakers now have limited possibilities for acquiring a comprehensive knowledge of the difficulties that farmers face on a national level. In this direction, the presented work proposes a new artificial intelligence-based pipeline to gain insights at country level regarding the farmers' demand for assistance in India.

View Article and Find Full Text PDF
Article Synopsis
  • Association analysis found 77 marker-trait associations (MTAs) linked to phosphorus use efficiency (PUE) traits in bread wheat, with 10 of these classified as high-confidence MTAs.*
  • Candidate-gene mining identified 13 potential genes that are crucial for PUE traits, specifically related to root system development and phosphorus uptake.*
  • The findings suggest that the identified MTAs and candidate genes can be utilized in breeding programs to create wheat varieties better adapted to low phosphorus conditions.*
View Article and Find Full Text PDF

Epigenetic processes in insect adaptation to environmental stress.

Curr Opin Insect Sci

February 2025

Plant-Insect Interaction Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi 110067, India. Electronic address:

Insects exhibit remarkable adaptability to a wide range of environmental stressors, including temperature fluctuations, pathogens, and changes in diet. This adaptability is often driven by epigenetic processes, which regulate gene expression without altering the underlying DNA sequence. This review provides a comprehensive overview of these epigenetic processes in insect adaptation, highlighting their impact on development, behaviour, and stress resilience.

View Article and Find Full Text PDF
Article Synopsis
  • Rice is a vital food source for over 50% of the global population, but its high glycemic index poses challenges for diabetic and obese individuals, necessitating the development of low-GI rice varieties through understanding starch biogenesis.
  • A study of 200 rice genotypes focused on starch content and categorized them into three groups based on amylose content, leading to the selection of specific genotypes for further analysis of resistant starch levels, protein content, and fatty acid profiles.
  • Results showed varying levels of resistant starch and fatty acids, with specific genotypes demonstrating significant enzymatic activity related to starch biosynthesis, highlighting genetic variations that can influence rice quality traits.
View Article and Find Full Text PDF
Article Synopsis
  • Sclerotinia sclerotiorum is a significant phytopathogen that causes Stem rot disease in Brassica crops, leading to decreased yields and oil quality, particularly in rapeseeds and Indian mustard.
  • The pathogen's hemibiotrophic nature and ability to persist in soil complicate management through traditional farming practices, making it essential to explore alternative strategies.
  • Recent advancements in omics technologies have identified various molecular components involved in the disease process, which may be utilized to enhance resistance in susceptible Brassica cultivars.
View Article and Find Full Text PDF

Anti-nutrient factors are inherently present in almost all major crops, which impede the absorption of crucial vitamins and minerals upon human consumption. The commonly found anti-nutrients in food crops are saponins, tannins, lectins, and phytates etc. Currently, there is a lack of computational server for identification of proteins that encode for anti-nutritional factors in plants.

View Article and Find Full Text PDF

Background: India's oilseed economy falls short of self-sufficiency and is supplemented by huge imports every year. Increasing national productivity of the major oilseeds is confronted with yield losses due to diverse biotic and abiotic stresses. The productivity of Indian mustard (Brassica juncea Linnaeus), belonging to the family Brassicaceae, is significantly reduced due to damage caused by mustard aphids (Lipaphis erysimi Kaltenbach, Hemiptera: Aphididae).

View Article and Find Full Text PDF
Article Synopsis
  • Cucumber (Cucumis sativus L.) is being researched for its potential to develop haploids and doubled haploids, which are key for creating pure inbred lines and speeding up plant breeding processes.
  • This study successfully induced haploid plants using gamma-irradiated pollen across different cucumber genotypes, focusing on the effects of varying doses of irradiation on plant development.
  • The findings highlight that using this method can significantly increase haploid production, which can improve genetic advancements and contribute to better agricultural practices and food security.
View Article and Find Full Text PDF

India's mango productivity is hindered by many factors but more importantly due to limited understanding of the genomic complexities behind regular bearing habit. This study is the first to quantify carbohydrate fractions, protein content, and macro and micronutrient storage pools, their transportation, and contributions to regular 'Totapuri' and alternate bearer 'Bombay Green' mango varieties during the 'off' year. Deep RNA sequencing was used to assess gene expression dynamics between buds and flowers of these varieties.

View Article and Find Full Text PDF
Article Synopsis
  • Long non-coding RNAs (lncRNAs) are vital for gene regulation in plants, affecting growth, development, flowering, and stress responses, particularly in cluster bean.
  • The study identified 3,309 differentially expressed lncRNAs (DE-lncRNAs), with many showing tissue-specific expression and involvement in floral development and stress response, validated through qRT-PCR.
  • Findings reveal complex regulatory networks among DE-lncRNAs, mRNAs, and miRNAs, contributing to our understanding of plant development, with data made publicly available in a dedicated database.
View Article and Find Full Text PDF
Article Synopsis
  • The study investigates the prevalence of vegetative insecticidal protein (vip) genes, specifically vip3-type genes, in Bacillus thuringiensis (Bt) isolates from diverse agro-climatic zones in India.
  • A PCR screening of 155 isolates shows a high percentage (70.32%) positive for partial vip3-type genes, with significant efficiency from specific primer sets when amplifying full-length genes.
  • The research highlights that Indian Bt isolates possess a substantial diversity of vip3 genes, with effective genes identified against specific pests, demonstrating their potential for resistance management in agriculture.
View Article and Find Full Text PDF

The review article summarizes the approaches and potential targets to address the challenges of anti-nutrient like phytic acid in millet grains for nutritional improvement. Millets are a diverse group of minor cereal grains that are agriculturally important, nutritionally rich, and the oldest cereals in the human diet. The grains are important for protein, vitamins, macro and micronutrients, fibre, and energy sources.

View Article and Find Full Text PDF

This study demonstrated the plant growth-promoting capabilities of native actinobacterial strains obtained from different regions of the rice plant, including the rhizosphere (FT1, FTSA2, FB2, and FH7) and endosphere (EB6). We delved into the molecular mechanisms underlying the beneficial effects of these plant-microbe interactions by conducting a transcriptional analysis of a select group of key genes involved in phytohormone pathways. Through in vitro screening for various plant growth-promoting (PGP) traits, all tested isolates exhibited positive traits for indole-3-acetic acid synthesis and siderophore production, with FT1 being the sole producer of hydrogen cyanide (HCN).

View Article and Find Full Text PDF

MicroRNAs are key players involved in stress responses in plants and reports are available on the role of miRNAs in drought stress response in rice. This work reports the development of a database, RiceMetaSys: Drought-miR, based on the meta-analysis of publicly available sRNA datasets. From 28 drought stress-specific sRNA datasets, we identified 216 drought-responsive miRNAs (DRMs).

View Article and Find Full Text PDF

White rust disease caused by a biotrophic oomycete is one of the most serious impediments in realizing the production potential of . Due to the obligate nature of the pathogen, R-gene-based resistance is unstable as the newer virulent races emerge quickly. For this, a deep understanding of the molecular basis of resistance is essential for developing durable resistant varieties.

View Article and Find Full Text PDF
Article Synopsis
  • CKX genes play a crucial role in regulating cytokinin levels, which are essential for plant growth and embryo/seed development in pigeonpea; however, there's limited research on their specific functions in this area.* -
  • This study discovered 18 CKX genes in pigeonpea, each differing in structure and expression, with many localized in the nucleus and unevenly distributed across certain chromosomes.* -
  • Analysis of gene expression patterns revealed that specific CKX genes may act as negative regulators of seed number per pod, indicating their potential for improving seed and biomass production in pigeonpea.*
View Article and Find Full Text PDF

Characterization of a novel cytosolic sesquiterpene synthase MpTPS4 from Mentha ×piperita as a bioresource for the enrichment of invaluable viridiflorol in mentha essential oil.

Int J Biol Macromol

October 2024

Plant Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow 226015, UP, India; CSIR-National Botanical Research Institute, Lucknow 226001, UP, India. Electronic address:

Article Synopsis
  • The research focuses on increasing the production of viridiflorol, a valuable sesquiterpene found in Mentha species, to enhance export revenue.
  • The study concentrated on the MpTPS4 enzyme, which exclusively synthesizes viridiflorol, and demonstrated a 9-25% increase in viridiflorol content by overexpressing this enzyme in M. ×piperita L.
  • Additionally, the research shows that viridiflorol can deter herbivores like Helicoverpa armigera and highlights the potential of biotechnology for improving the production of this commercially important compound.
View Article and Find Full Text PDF

Rice grown under deepwater ecosystem is considered to be natural farming and hence they are considered to be input efficient. Thus, to identify gene responsible for nutritional content under natural conditions, a genome-wide association study (GWAS)was performed. GWAS identified single nucleotide polymorphisms (SNPs) significantly associated with various nutritional quality traits such as Zn (mg/kg), Fe (mg/kg), Protein (%), Oil (%), Amylose (%), Starch (%), Phytic acid (%), Phenol (%) and TDF (%) in 184 deepwater rice accessions evaluated over 2 consecutive years.

View Article and Find Full Text PDF

Background: Bemisia tabaci, a significant agricultural pest in Asia, contains distinct genetic groups, Asia-1 and Asia II-1. Understanding its reproductive biology, particularly the role of ejaculatory bulb proteins (EBPs) in mating, is crucial. However, EBPs in B.

View Article and Find Full Text PDF