Metagenome analysis from the sediment of river Ganga and Yamuna: In search of beneficial microbiome.

Beneficial microbes are all around us and it remains to be seen, whether all diseases and disorders can be prevented or treated with beneficial microbes. In this study, the presence of various beneficial bacteria were identified from the sediments of Indian major Rivers Ganga and Yamuna from nine different sites using a metagenomic approach. The metagenome sequence analysis using the Kaiju Web server revealed the presence of 69 beneficial bacteria.

Corrigendum: Phloroglucinol-Mediated Hsp70 Production in Crustaceans: Protection against in and .

[This corrects the article DOI: 10.3389/fimmu.2018.

Modeling pre-spawning fitness and optimal climate of spotted snakehead Channa punctata (Bloch, 1793) from a Gangetic floodplain wetland of West Bengal, India.

The spawning and well-being of fish in an ecosystem are closely linked to climatic cues, viz., temperature and rainfall. Reduced fitness can affect the reproductive performance and lead to skipped spawning.

Do acute hepatopancreatic necrosis disease-causing PirAB toxins aggravate vibriosis?

Gram-negative marine bacterium is an important aquatic pathogen and has been demonstrated to be the causative agent of acute hepatopancreatic necrotic disease (AHPND) in shrimp aquaculture. The AHPND-causing strains contain a pVA1 plasmid encoding the binary PirA and PirB toxins, are the primary virulence factor that mediates AHPND and mortality in shrimp. Since PirAB toxins are secreted extracellularly, one can hypothesize that PirAB toxins would aggravate vibriosis in the aquatic environment.

Metagenomic study focusing on antibiotic resistance genes from the sediments of River Yamuna.

Antibiotic resistance is one of the major health concerns of the present century. The direct discharge of urban sewage, hospital effluents, and pharmaceutical wastes increases the concentration of antibiotics in riverine ecosystems. This provides selection pressure for the development of novel antibiotic-resistant strains.

Biofloc-Based Enhanced Survival of Upon AHPND-Causing Challenge Is Partially Mediated by Reduced Expression of Its Virulence Genes.

The biofloc system is a relatively new aquaculture technology that offers practical solution to maintain culture water quality by recycling nutrients and improves the health status and resistance of shrimps against microbial infection, yet the mode of action involved remains unclear. This study aimed to unravel the underlying mechanism behind the protective effect of a biofloc system using and acute hepatopancreatic necrosis disease (AHPND)-causing M0904 strain as a host-pathogen model. The results showed that a biofloc system maintained at a C/N ratio of 15, improves the water quality and contributes to the nutrition of cultured animals as bioflocs might serve as an additional protein source.

Broodstock development and captive maturation of hilsa (Tenualosa ilisha) in a brackishwater pond-based system.

Successful captive broodstock development of clupeid fish, hilsa, is one of the major challenges for the artificial propagation of this economically valued fish. The present study provides novel insights into the pond-reared captive broodstock development of hilsa using artificial feed. In the present study, wild collected hilsa fry (weight 1.

Population structure of Nile tilapia and its impact on fisheries of a tropical impacted reservoir, Central India.

Length-frequency data of Nile tilapia (Oreochromis niloticus) was studied using 1250 specimens of both sexes combined with maximum length and weight of 44.5 cm (TL) and 1050 g respectively through gill net catch at different landing sites of Halali Reservoir, Madhya Pradesh. The data were grouped into classes of 10-mm intervals and various parameters of population dynamics of fish were analysed and computed using the FiSAT-II software package (FAO-ICLARM Stock Assessment Tools).

Transcriptomic responses to pollution in natural riverine environment in Rita rita.

Aquatic pollution is one of the most common threats to the ecological health of aquatic ecosystems and its biota. Fish as lower vertebrates are excellent model to study the impact and responses of aquatic pollution. In fish, gill is the main organ indicator of whole animal health as it comes in contact with the surrounding water and absorbs many pollutants and contaminants; therefore, investigations on alterations in fish gill at transcriptome level could provide newer insights to the stress response mechanism(s) and pathways.

Health risks from trace elements in muscles of some commonly available fish in Australia and India.

The levels of trace elements (As, Hg, Cr, Cd, Pb, Co, Ni, Cu, Mn and Zn) in commercially important fish species sampled from fish markets of Adelaide, Australia; canned fish from South Australian supermarkets; and fish markets of West Bengal, India were determined by inductively coupled plasma mass spectrometry (ICP-MS) after microwave digestion. Mercury was determined by using triple quadrupole ICP-MS. The accuracy of the methods was assessed with a certified standard reference material (NRCC-DORM-3 dogfish protein), and the results were compared with values reported in the literature.

Assessment of urban growth effects on green space and surface temperature in Doon Valley, Uttarakhand, India.

This study aims to examine the spatio-temporal urban expansion pattern and its impacts on green space variation as well as thermal behavior in Doon valley over the last two decades during 2000 and 2019. Landsat 5 and Landsat 8 images of February and May month of two study years 2000 and 2019 were used for the analysis. The land use change analysis revealed notable outgrowth of urbanization with 184% increase in Doon valley during 2000-2019.

A comparative metabolomics study on anadromous clupeid Tenualosa ilisha for better understanding the influence of habitat on nutritional composition.

Introduction: Fish inhabiting different aquatic habitats adapts to the environment by metabolomic readjustments. Understanding the combined activities of all the metabolic pathways (metabolome) helps in better understanding the complex interactions between gene and environment.

Objectives: The anadromous migratory Tenualosa ilisha is a high value food fish comprising the dominant fishery of the rivers Padma and Hooghly.

Cloning, characterization and ontogenetic expression profile of RAG-2 and IgM in angelfish (Pterophyllum scalare).

Early larval developmental stages of fish are highly susceptible to opportunistic pathogens until the complete maturation of the lymphoid organs. Knowledge of the expression pattern of important markers of adaptive immune system during the ontogenetic development is essential before vaccinating the fish. In the present study, Pterophyllum scalare (angelfish) was taken to explore the relative expression profile of developmental markers of adaptive immunity, recombination activating gene-2 (RAG-2) and immunoglobulin M (IgM).

Pattern of the state of eutrophication in the floodplain wetlands of eastern India in context of climate change: a comparative evaluation of 27 wetlands.

The floodplain wetlands in different regional settings vary with time and space in terms of function and geomorphological diversity. In recent decades, these eco-sensitive waterbodies have been exposed to a wide range of anthropogenic threats and climatic changes. Therefore, assessment of these ecological and environmental threats is prerequisite to understand the state of ecosystem and to develop a sustainable management strategy for conservation of wetland biodiversity and fisheries enhancement.

De novo transcriptome analysis of halotolerant bacterium Staphylococcus sp. strain P-TSB-70 isolated from East coast of India: In search of salt stress tolerant genes.

In the present study, we identified salt stress tolerant genes from the marine bacterium Staphylococcus sp. strain P-TSB-70 through transcriptome sequencing. In favour of whole-genome transcriptome profiling of Staphylococcus sp.

Genetic differentiation and phylogenetic relationship of 11 Asian Sisorinae genera (Siluriformes: Sisoridae) with new record of .

Studies on systematics have been largely restricted to morphological data with few studies on examination of phylogenetic relations. However, no study has been done to evaluate genetic distance of the genera under sub-family and detailed phylogenetic relations within it. We used nuclear recombination activating 2 () gene and mitochondrial cytochrome c oxidase I () gene from 64 species to examine genetic differentiation and phylogenetic relationships within 11 Asian genera.

Status of pesticide residues in water, sediment, and fishes of Chilika Lake, India.

Chilika Lake is the largest coastal lagoon in Asia and the second largest in the world covering an area of 1100 km and spread over three districts of Odisha state of India. It is the first Indian wetland designated as a wetland of international importance under the Ramsar Convention in 1981. The lake ecosystem sustains large and diversified resources of plants and animals including fisheries.

Proteomic and transcriptomic changes in rat liver following oral feeding of formaldehyde.

Formaldehyde (FA), a ubiquitous volatile organic compound present in a wide range of resources, is a hazardous chemical and human carcinogen. Contamination of FA in food, especially perishable commodities like fish and meat, is a major source of exposure, although it is not recommended for use in food and food products owing to its carcinogenicity. Effects of oral feeding of FA have been studied by evaluating general health, haematology and clinical chemistry in rat.

Nutritional profiling of hilsa (Tenualosa ilisha) of different size groups and sensory evaluation of their adults from different riverine systems.

Nutritional composition of hilsa, Tenualosa ilisha, of different size groups was analyzed to study variations in their composition with the progression of growth, and to correlate it with the flavor of adult hilsa (>800 g size) collected from different riverine systems (Hooghly and Padma). The amino acid analysis revealed significantly higher arginine (P < 0.01), methionine (P < 0.

PirAB Toxin Binds to Epithelial Cells of the Digestive Tract and Produce Pathognomonic AHPND Lesions in Germ-Free Brine Shrimp.

Acute hepatopancreatic necrosis disease (AHPND), a newly emergent farmed penaeid shrimp bacterial disease originally known as early mortality syndrome (EMS), is causing havoc in the shrimp industry. The causative agent of AHPND was found to be a specific strain of bacteria, e.g.

Phloroglucinol Treatment Induces Transgenerational Epigenetic Inherited Resistance Against Infections and Thermal Stress in a Brine Shrimp () Model.

Emerging, infectious diseases in shrimp like acute hepatopancreatic necrosis disease (AHPND) caused by and mortality caused by other species such as are worldwide related to huge economic losses in industrial shrimp production. As a strategy to prevent disease outbreaks, a plant-based phenolic compound could be used as a biocontrol agent. Here, using the brine shrimp () as a model system, we showed that phloroglucinol treatment of the parental animals at early life stages resulted in transgenerational inherited increased resistance in their progeny against biotic stress, i.

Isolation, characterization and growth kinetics of phenol hyper-tolerant bacteria from sewage-fed aquaculture system.

Pollution of aquatic resources is increasing day-by-day, and phenolic compounds are common pollutants negatively impacting aquatic biodiversity and production. This study aimed at isolation of phenol hyper-tolerant bacteria from polluted aquaculture resource so that they might be useful in aquaculture systems. Four phenol hyper-tolerant bacterial strains were isolated from sewage fed East Kolkata Wetlands, a Ramsar site.

Structural Characterization of Open Reading Frame-Encoded Functional Genes from Tilapia Lake Virus (TiLV).

In recent years, large-scale mortalities are observed in tilapia due to infection with a novel orthomyxo-like virus named, tilapia lake virus (TiLV) which is marked to be a severe threat to universal tilapia industry. Currently, there are knowledge gaps relating to the antiviral peptide as well as there are no affordable vaccines or drugs available against TiLV yet. To understand the spreading of infection of TiLV in different organs of Oreochromis niloticus, RT-PCR analysis has been carried out.

Elucidating the molecular interaction of Zebrafish () peptidoglycan recognition protein 2 with diaminopimelic acid and lysine type peptidoglycans using approaches.

Peptidoglycan recognition proteins (PGRPs) belong to the family of pattern recognition receptor, represent the major constituent of innate immunity. Although PGRPs are structurally conserved through evolution, their involvement in innate immunity is different in vertebrates and invertebrates. They are highly specific towards recognition of ligands and can hydrolyze bacterial peptidoglycans (PGNs).