595 results match your criteria: "IBB-Institute for Bioengineering and Biosciences[Affiliation]"

Central nervous system vascularization in human embryos and neural organoids.

Cell Rep

December 2024

Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA; Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI, USA. Electronic address:

Article Synopsis
  • Neural organoids from human pluripotent stem cells are emerging as powerful tools for exploring CNS development, disease, and drug interactions.
  • Despite their promise, many studies on CNS organoids lack effective blood vessel systems, limiting their applicability.
  • The review examines current knowledge on vascular development in various CNS regions and emphasizes the need for bioengineering advancements to create more functional vascularized organoid models for research purposes.
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Editorial: Functional nanogels and multicomponent supramolecular systems.

Front Chem

November 2024

Laboratório Nanotecnologia e Engenharia de Processos-NEP, Universidade de São Paulo, Lorena, São Paulo, Brazil.

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Article Synopsis
  • 2-Phenylbenzimidazole-5-sulfonic acid (PBSA) and disodium phenyl dibenzimidazole tetrasulfonate (DPDT) are UV sunscreen filters that can release harmful high-energy photons, necessitating the use of fluorescence quenchers in formulations.
  • Troxerutin is evaluated as a fluorescence quencher for both PBSA and DPDT, with experiments conducted on both solutions and a human skin mimic to benchmark their photodynamics.
  • The study reveals that adding troxerutin significantly reduces the fluorescence lifetime of PBSA and DPDT, indicating that it quenches fluorescence via Dexter energy transfer (DET) or Förster resonance energy transfer (FRET) mechanisms.
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Recent Advances in Calixarene-Based Fluorescent Sensors for Biological Applications.

Sensors (Basel)

November 2024

IBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisboa, Portugal.

Article Synopsis
  • Calixarenes, a type of macrocyclic compound, have been combined with various fluorophores to create fluorescent probes for a wide range of applications, particularly in biological fields.
  • The review highlights advancements in calixarene-based fluorescent sensors since 2009, focusing on their ability to detect important biological ions and specific biomolecules like amino acids and drugs.
  • Additionally, the review discusses the role of calixarene-containing nanoparticles and nanoaggregates in imaging and drug delivery, while offering conclusions and future insights into the development of these technologies.
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Targeting Vascular Endothelial Growth Factor Receptor 2 (VEGFR-2): Latest Insights on Synthetic Strategies.

Molecules

November 2024

Centro de Química de Coimbra, Institute of Molecular Sciences (CQC-IMS), Departamento de Química, Faculdade de Ciências e Tecnologia, University of Coimbra, 3004-535 Coimbra, Portugal.

Vascular endothelial growth factor receptor 2 (VEGFR-2) is a crucial mediator of angiogenesis, playing a pivotal role in both normal physiological processes and cancer progression. Tumors harness VEGFR-2 signaling to promote abnormal blood vessel growth, which is a key step in the metastasis process, making it a valuable target for anticancer drug development. While there are VEGFR-2 inhibitors approved for therapeutic use, they face challenges like drug resistance, off-target effects, and adverse side effects, limiting their effectiveness.

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Lipid-Based Nanoformulations for Drug Delivery: An Ongoing Perspective.

Pharmaceutics

October 2024

Department of Bioengineering, iBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, University of Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal.

Oils and lipids help make water-insoluble drugs soluble by dispersing them in an aqueous medium with the help of a surfactant and enabling their absorption across the gut barrier. The emergence of microemulsions (thermodynamically stable), nanoemulsions (kinetically stable), and self-emulsifying drug delivery systems added unique characteristics that make them suitable for prolonged storage and controlled release. In the 1990s, solid-phase lipids were introduced to reduce drug leakage from nanoparticles and prolong drug release.

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Many species of the genus are known to be highly tolerant to solvents and other environmental stressors. Based on phylogenomic and comparative genomic analyses, several species were recently transferred to a new genus named . Because of their unique enzymatic machinery, these strains are being discussed as novel biocatalysts in biotechnology.

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Comparison of two metabolomics-platforms to discover biomarkers in critically ill patients from serum analysis.

Comput Biol Med

January 2025

ISEL - Instituto Superior de Engenharia de Lisboa, Instituto Politécnico de Lisboa, Rua Conselheiro Emídio Navarro 1, 1959-007, Lisbon, Portugal; IBB-Institute for Bioengineering and Biosciences, The Associate Laboratory Institute for Health and Bioeconomy (i4HB), Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001, Lisbon, Portugal. Electronic address:

Serum metabolome analysis is essential for identifying disease biomarkers and predicting patient outcomes in precision medicine. Thus, this study aims to compare Ultra-High Performance Liquid Chromatography-High-Resolution Mass Spectrometry (UHPLC-HRMS) with Fourier Transform Infrared (FTIR) spectroscopy in acquiring the serum metabolome of critically ill patients, associated with invasive mechanical ventilation (IMV), and predicting death. Three groups of 8 patients were considered.

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Purpose: The Achilles tendon is a common injury site, but anatomical risk factors for injury are relatively unexplored in the literature. This study aimed to evaluate whether changes in free tendon length would influence the results of a simulated rupture of the Achilles tendon.

Methods: Using a previously validated 3D finite element model of the free and aponeurotic Achilles tendon as a basis, two additional finite element models with 25% decreased and increased free tendon lengths were created.

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Analysis of six consecutive waves of ICU-admitted COVID-19 patients: key findings and insights from a Portuguese population.

Geroscience

November 2024

Intensive Care Department, ULSSJ - Unidade Local de Saúde São José, Rua José António Serrano, 1150-199, Lisbon, Portugal.

Identifying high-risk patients, particularly in intensive care units (ICUs), enhances treatment and reduces severe outcomes. Since the pandemic, numerous studies have examined COVID-19 patient profiles and factors linked to increased mortality. Despite six pandemic waves, to the best of our knowledge, there is no extensive comparative analysis of patients' characteristics across these waves in Portugal.

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Transforming cancer treatment: The potential of nanonutraceuticals.

Int J Pharm

December 2024

Center for Marine Sciences (CCMAR), University of Algarve, Gambelas Campus, Faro 8005-139, Portugal; Department of Chemistry and Pharmacy, Faculty of Sciences and Technology, University of Algarve, Gambelas Campus, Faro 8005-139, Portugal; iBB-Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Lisboa 1049-001, Portugal; Associate Laboratory i4HB-Institute for Health and Bioeconomy at Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, Lisboa 1049-001, Portugal. Electronic address:

Chemotherapy in the management of cancer is constrained by limitations like off-target effects, poor bioavailability, and dose-dependent toxicity. Nutraceuticals have been explored as an innovative strategy to overcome chemotherapy drawbacks.However, the clinical utility of nutraceuticals is restricted due to their complex structures, less water solubility, reduced stability, decreased bioavailability and more obstacles in the gastrointestinal tract.

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Hypertrophic cardiomyopathy (HCM) is the most prevalent inherited cardiomyopathy and a leading cause of sudden death. Genetic testing and familial cascade screening play a pivotal role in the clinical management of HCM patients. However, conventional genetic tests primarily focus on the detection of exonic and canonical splice site variation.

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Systemic lupus erythematosus (SLE or lupus) is an immune-mediated disease associated with substantial medical burden. Notably, lupus exhibits a striking female bias, with women having significantly higher susceptibility compared to men, up to 14-fold higher in some ethnicities. Supernumerary X chromosome syndromes, like Klinefelter (XXY) and Triple X syndrome (XXX), also present higher SLE prevalence, whereas Turner syndrome (XO) displays lower prevalence.

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The oleaginous yeast species Rhodotorula toruloides is a promising candidate for applications in circular bioeconomy due to its ability to efficiently utilize diverse carbon sources being tolerant to cellular stress in bioprocessing. Previous studies including genome-wide analyses of the multi-stress tolerant strain IST536 MM15, derived through adaptive laboratory evolution from a promising IST536 strain for lipid production from sugar beet hydrolysates, suggested the occurrence of significant modifications in the cell wall. In this study, the cell wall integrity and carbohydrate composition of those strains was characterized to gain insights into the physicochemical changes associated to the remarkable multi-stress tolerance phenotype of the evolved strain.

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Polymeric scaffolds play a pivotal role in tissue engineering (TE) and regenerative medicine strategies, as they offer the possibility to closely mimic the architectural features of the native tissues' extracellular matrix (ECM) and support cell performance both in vitro and in vivo, creating a favourable regenerative microenvironment [...

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Insights into the Dual Anticancer and Antibacterial Activities of Composites Based on Silver Camphorimine Complexes.

J Funct Biomater

August 2024

Centro de Química Estrutural, Institute of Molecular Sciences and Departamento de Engenharia Química, Instituto Superior Técnico, Universidade de Lisboa, Avenida António José de Almeida, n.º 12, 1000-043 Lisboa, Portugal.

Hydroxyapatite (HAp) is a widely used biocompatible material in orthopedic composite preparations. However, HAp composites that exhibit both anticancer and antibacterial activities through bioactive coordination complexes are relatively rare. To explore orthopedic applications, we blended several silver camphorimine compounds with HAp to create [Ag(I)] composites.

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Enhanced osteointegration and osteogenesis of osteoblast cells by laser-induced surface modification of Ti implants.

Nanomedicine

November 2024

Biomedical Research Centre, Sumy State University, R-Korsakova Str., 40007 Sumy, Ukraine; Institute of Atomic Physics and Spectroscopy, University of Latvia, Jelgavas Str., LV-1004 Riga, Latvia. Electronic address:

Dental and orthopedic implants have become routine medical technologies for tooth replacement and bone fixation. Despite significant progress in implantology, achieving sufficient osseointegration remains a challenge, often leading to implant failure over the long term. Nanotechnology offers the potential to mimic the natural patterns of living tissues, providing a promising platform for tissue engineering and implant surface design.

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Involvement of extracellular vesicle microRNA clusters in developing healthy and Rett syndrome brain organoids.

Cell Mol Life Sci

September 2024

Department of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience (MHeNs), Faculty of Health, Medicine and Life Sciences (FHML), Maastricht University, Maastricht, 6200, MD, The Netherlands.

Rett syndrome (RTT) is a neurodevelopmental disorder caused by de novo mutations in the MECP2 gene. Although miRNAs in extracellular vesicles (EVs) have been suggested to play an essential role in several neurological conditions, no prior study has utilized brain organoids to profile EV-derived miRNAs during normal and RTT-affected neuronal development. Here we report the spatiotemporal expression pattern of EV-derived miRNAs in region-specific forebrain organoids generated from female hiPSCs with a MeCP2:R255X mutation and the corresponding isogenic control.

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Advancing cancer therapeutics: Integrating scalable 3D cancer models, extracellular vesicles, and omics for enhanced therapy efficacy.

Adv Cancer Res

September 2024

Department of Bioengineering and iBB - Institute for Bioengineering and Biosciences at Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal; Associate Laboratory i4HB - Institute for Health and Bioeconomy at Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal. Electronic address:

Article Synopsis
  • * Three-dimensional (3D) models, like tumor spheroids and organoids, offer better mimicry of cancer biology and drug resistance, but their widespread use depends on scalable production methods for efficient drug testing.
  • * The integration of extracellular vesicles as drug delivery systems with these advanced 3D models and omics profiling can enhance our understanding of cancer treatments and improve therapeutic outcomes.
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The periodontium is a complex hierarchical structure composed of alveolar bone, periodontal ligament, cementum, and gingiva. Periodontitis is an inflammatory disease that damages and destroys the periodontal tissues supporting the tooth. Periodontal therapies aim to regenerate the lost tissues, yet current treatments lack the integration of multiple structural/biochemical instructive cues to induce a coordinated regeneration, which leads to limited clinical outcomes.

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Methanol is a promising feedstock for biomanufacturing, but the efficiency of methanol-based bioprocesses is limited by the low rate of methanol utilization pathways and methanol toxicity. Yeast diversity is an attractive biological resource to develop efficient bioprocesses since any effort with strain improvement is more deserving if applied to innate robust strains with relevant catabolic and biosynthetic potential. The present study is in line with such rational and describes the isolation and molecular identification of seven isolates of the methylotrophic species Candida boidinii from waters derived from the traditional curation of olives, in different years, and from contaminated superficial soil near fuel stations.

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Isolation from the Nasal Cavity of a Cat with Chronic Rhinitis.

Vet Sci

August 2024

CIISA-Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal.

The genus includes ubiquitous bacteria frequently described as animal and human opportunistic pathogens. A 9-year-old cat was referred for rhinoscopy at the Veterinary Hospital of the Faculty of Veterinary Medicine, University of Lisbon, Portugal, for an investigation of the chronic respiratory signs. Upon rhinoscopy, nasal and nasopharyngeal discharge were observed, and the nasal turbinates showed signs of inflammation.

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Fabrication and Characterization of Porous PEGDA Hydrogels for Articular Cartilage Regeneration.

Gels

June 2024

Department of Bioengineering, iBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.

Functional articular cartilage regeneration remains an unmet medical challenge, increasing the interest for innovative biomaterial-based tissue engineering (TE) strategies. Hydrogels, 3D macromolecular networks with hydrophilic groups, present articular cartilage-like features such as high water content and load-bearing capacity. In this study, 3D porous polyethylene glycol diacrylate (PEGDA) hydrogels were fabricated combining the gas foaming technique and a UV-based crosslinking strategy.

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