A novel monoclonal antibody reveals the enrichment of NADPH oxidase 5 in human splenic endothelial cells.

Members of the NOX/DUOX family of NADPH oxidases are responsible for regulated ROS production in diverse cells and tissues. Detection of NOX/DUOX proteins at the protein level remains an important challenge in the field. Here we report the development and characterization of a novel anti-NOX5 monoclonal antibody, which recognizes the human NOX5 protein in both Western blot, immunocytochemistry, and histochemistry applications.

Imaging Intracellular HO with the Genetically Encoded PerFRET and OxyFRET Probes.

Hydrogen peroxide (HO) is an important signaling intermediate with various regulatory and effector functions. Despite its significance, the subcellular organization of HO signals is poorly understood. Introducing novel techniques for the intracellular detection of HO would be essential for a more complete understanding of its role in cellular signaling.