Transcriptomic and metabonomic insights into the biocontrol mechanism of Trichoderma asperellum M45a against watermelon Fusarium wilt.

Watermelon (Citrullus lanatus) is one of the most popular fruit crops. However, Fusarium wilt (FW) is a serious soil-borne disease caused by Fusarium oxysporum f. sp.

Protein kinase Ime2 is associated with mycelial growth, conidiation, osmoregulation, and pathogenicity in Fusarium oxysporum.

Fusarium oxysporum f.sp. niveum is one of the most serious diseases impairing watermelon yield and quality.

Comparative transcriptomic analysis of the super hybrid rice Chaoyouqianhao under salt stress.

Background: Soil salinization is a threat to food security. China is rich in saline land resources for potential and current utilization. The cultivation and promotion of salt-tolerant rice varieties can greatly improve the utilization of this saline land.

C/EBPα Regulates 1 and 2 Trehalase-Related Bt Resistance in (L.).

Trehalase regulates energy metabolism in insects by converting trehalose into two glucose molecules. High amounts of trehalase are critical for insect flight and larval stress resistance. However, whether trehalase participates in the development of pesticide resistance remains unclear.

Reprogramming of the Epigenome and Transcriptome in Response to Cold Stress.

Low temperature is a key factor limiting the rubber plantation extending to high latitude area. Previous work has shown that cold-induced DNA demethylation was coordinated with the expression of cold-responsive () genes in . In this work, reduced representation bisulphite sequencing analysis of showed that cold treatment induced global genomic DNA demethylation and altered the sequence contexts of methylated cytosines, but the levels of mCG methylation in transposable elements were slightly enhanced by cold treatment.

Comparative transcriptome analysis of the cold resistance of the sterile rice line 33S.

Rice (Oryza sativa L.) is one of the most important species for food production worldwide. Low temperature is a major abiotic factor that affects rice germination and reproduction.

Superior rice variety YJ144 with improved rice blast resistance, yield, and quality achieved using molecular design and multiple breeding strategies.

Unlabelled: Yanfeng 47 (YF47) is an elite rice variety cultivated in China on nearly 2 million hectares over the past 20 years. However, YF47 is highly susceptible to rice blast (), one of the most destructive rice diseases. In this study, we developed novel TPAP (tetra-primer ARMS-PCR) functional markers for the genes , , and , all of which afford broad-spectrum resistance to blast.

Genome editing with type II-C CRISPR-Cas9 systems from Neisseria meningitidis in rice.

Two type II-C Cas9 orthologs (Nm1Cas9 and Nm2Cas9) were recently identified from Neisseria meningitidis and have been extensively used in mammalian cells, but whether these NmCas9 orthologs or other type II-C Cas9 proteins can mediate genome editing in plants remains unclear. In this study, we developed and optimized targeted mutagenesis systems from NmCas9s for plants. Efficient genome editing at the target with N GATT and N CC protospacer adjacent motifs (PAMs) was achieved with Nm1Cas9 and Nm2Cas9 respectively.

Effects of Grain Shape Genes Editing on Appearance Quality of Erect-Panicle Geng/Japonica Rice.

Long grain geng/japonica rice has a higher market preference due its excellent appearance quality. The dense and erect-panicle 1 (dep1) gene has been widely used in the breeding of high-yielding geng/japonica rice cultivars in China. However, this gene causes short and round grain shape thus making it less attractive in global rice markets.

Fumarylacetoacetate hydrolase is required for fertility in rice.

The rice OsFAH gene functions identically to that of Arabidopsis SSCD1 encoding FAH. Loss of OsFAH causes rice sterility. Fumarylacetoacetate hydrolase (FAH) is the last enzyme in the tyrosine (Tyr) degradation pathway that is crucial for animals.

NIR spectroscopy coupled with chemometric algorithms for the prediction of cadmium content in rice samples.

Fast determination of heavy metals is necessary and important to ensure the safety of crops. The potential of near-infrared spectroscopy coupled with chemometric technology for quantitative analysis of cadmium in rice was investigated. A total of 825 rice samples were collected and scanned by NIRS.

Identification of QTLs associated with the anaerobic germination potential using a set of Oryza nivara introgression lines.

Background: Rice (Oryza sativa L.) is an important crop and a staple food for half of the population around the world. The recent water and labor shortages are encouraging farmers to shift from traditional transplanting to direct-seeding.

Mass spectrometry-based metabolomics investigation on two different indica rice grains (Oryza sativa L.) under cadmium stress.

Cadmium is a toxic environmental pollutant that is readily absorbed by rice grains and poses serious threats to human health. The selection and breeding of rice varieties with low cadmium accumulation is one of the most economical and ecological methods to reduce cadmium exposure. In this study, two different indica rice grains under cadmium stress were subjected to mass spectrometry-based metabolomics analysis for the first time.

[Effects of Different Rotation Patterns of Oil-Rice on Methane and Nitrous Oxide Emissions in Rice Fields].

A field experiment was carried out to investigate the effects of different rice-rape rotation systems on methane and nitrous oxide emissions, which were measured using the static chamber/gas chromatography method, prediction of their global warming potentials (GWP), and greenhouse gas intensity (GHGI) in paddy fields. The results showed that the average cumulative emissions of CHfrom a double cropping paddy field, single season rice field (including middle or late), rape field, and leisure land were 135.25, 55.

Scheduling of nitrogen fertilizer topdressing during panicle differentiation to improve grain yield of rice with a long growth duration.

Topdressing at panicle differentiation (PF) according to soil fertility and regularity of rice nutrient absorption is an important agronomic practice used in cultivation of rice cultivars with a long growth duration. We studied the impacts of timing of nitrogen fertilizer application during PF on photosynthesis and yield-related agronomic traits in 'Y-Liang-You 900' and 'Y-Liang-You 6', which are representative rice cultivars with a long growth duration. Data for two years showed that timing of topdressing application during PF affected panicles per unit area, percentage grain set, spikelets per panicle, and leaf photosynthetic traits during the grain-filling period.

The Cds.71 on May Act as a Mutation Hotspot to Originate a TGMS Trait in Indica Rice Cultivars.

The gene , which controls thermo-sensitive genic male sterility (TGMS), has been widely used in two-line hybrid rice breeding in China. The lines have two sources, namely, AnnongS-1 (AnS) and Zhu1S (ZhS) and, interestingly, are commonly subject to an alteration at cds.71.

QTL mapping and candidate gene analysis of cadmium accumulation in polished rice by genome-wide association study.

Cadmium (Cd) accumulation in rice is a serious threat to food safety and human health. Breeding rice varieties with low Cd accumulation is one of the most effective approaches to reducing health risks from Cd-polluted rice. However, the genetic basis of Cd accumulation in grains, especially in indica rice varieties, has not been fully elucidated.

Rice Gene Improves Drought Tolerance by Modulating Polyamine Biosynthesis Depending on Abscisic Acid and ROS Levels.

Drought is a serious problem, which causes heavy yield losses for rice. Heat-shock factors (HSFs) had been implicated in tolerance to drought and high temperature. However, there has not been much functional characterization and mechanism clarification in rice.

CYP71D8L is a key regulator involved in growth and stress responses by mediating gibberellin homeostasis in rice.

Phytohormones are crucial in the regulation of plant growth and development, and in responses to adverse environments. Multiple cytochrome P450 monooxygenases (CYP450s) are involved in the biosynthesis and catabolism of phytohormones. Here, we report that a CYP450 member of the CYP71 clan in rice, OsCYP71D8L, participates in the control of multiple agronomic traits and abiotic stress responses by affecting gibberellin (GA) and cytokinin (CK) homeostasis.

Mutation at Different Sites of Metal Transporter Gene Affects Cd Accumulation and Related Agronomic Traits in Rice ( sativa L.).

is a key gene involved in the control of the uptake of Cd, Mn, and other metal ions by rice root cells. The functional deficiency of this gene can significantly reduce the accumulation of Cd in rice grains, but the effects of its mutation on agronomic traits such as yield and quality have not been investigated comprehensively yet. In the present study, three Huanghuazhan-based mutants [ (), (), and ()] were obtained using clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) technology.

WGCNA Analysis of Salt-Responsive Core Transcriptome Identifies Novel Hub Genes in Rice.

Rice, being a major staple food crop and sensitive to salinity conditions, bears heavy yield losses due to saline soil. Although some salt responsive genes have been identified in rice, their applications in developing salt tolerant cultivars have resulted in limited achievements. Herein, we used bioinformatic approaches to perform a meta-analysis of three transcriptome datasets from salinity and control conditions in order to reveal novel genes and the molecular pathways underlying rice response to salt.

Identification of putative drought-responsive genes in rice using gene co-expression analysis.

Drought is one of the major abiotic stresses causing yield losses and restricted growing area for several major crops. Rice being a major staple food crop and sensitive to water-deficit conditions bears heavy yield losses due to drought stress. To breed drought tolerant rice cultivars, it is of interest to understand the mechanisms of drought tolerance.

Potential for high yield with increased seedling density and decreased N fertilizer application under seedling-throwing rice cultivation.

Fertilizer application for rice production has increased significantly in southern China to raise yields, but has led to problems with lodging, quality decline and environmental pollution. Therefore, research on fertilizer-saving cultivation technologies for high-yielding rice is necessary. A two-factor experiment was conducted to evaluate the effects of seedling-addition treatment (SAT) and nitrogen-saving treatment (NST) on yield formation and nitrogen absorption of individual plants and plant groups under the seedling-throwing cultivation system.

Conversion of a rice CMS maintainer into a photo- or thermo-sensitive genetic male sterile line.

A maintainer line of 3-line hybrid rice commonly presents a certain genetic distance to a 2-line restorer line, but in many cases, 2-line restorer lines present defects upon recovery of the object cytoplasmic male sterile (CMS) line of the maintainer line, which impedes the utilization of their heterosis. Here, we report a strategy and an example of converting a maintainer into a photoperiod/temperature-sensitive genic male sterile (P/TGMS) line with an almost identical genetic background, thus maximizing the heterosis. Firstly, through treatment of maintainer line T98B with C-γ irradiation, we identified the TGMS line T98S, which is sterile at higher temperatures and fertile at lower temperatures.

Synthesis of Carbon Dots from Pear Juice for Fluorescence Detection of Cu Ion in Water.

In this work, a facile green hydrothermal method was developed for the synthesis of carbon dots with fluorescent property using pear juice as raw materials. The synthesized carbon dots were characterized by UV-vis, fluorescence and transmission electron microscopic techniques. The synthesized conditions were optimized and the obtained carbon dots exhibited an average size at 10 nm with bright emission centered at 455 nm (blue color) under UV light with the excitation wavelength at 360 nm.