1 results match your criteria: "Hunan Province Geriatric Hospital Changsha 410016[Affiliation]"
Bevacizumab induces A549 cell apoptosis through the mechanism of endoplasmic reticulum stress in vitro.
Int J Clin Exp Pathol
April 2016
Department of Respiratory Medicine, Hunan Province Geriatric Hospital Changsha 410016, China ; Institute of Respiratory Disease, Hunan Province Geriatric Hospital Changsha 410016, China.
Aims: To observe the effect of bevacizumab on human A549 cells and explore its mechanism.
Methods: After different concentrations (0 μM, 1 μM, 5 μM, 25 μM) of bevacizumab treating in A549 cells, CCK8 assay detect the impact of bevacizumab on A549 cell proliferation and flow cytometry determine the effect of bevacizumab on human A549 cells apoptosis. Real-time PCR and Western blotting detect the changing expression of the target gene (CHOP, caspase-4, IRE1, XBP-1) on mRNA and Protein level.