Mechanism of medical hemorrhoid gel in relieving pruritus ani via inhibiting the activation of JAK2/STAT3 pathway.

Background: Pruritus ani (PA), a neurofunctional dermatosis, is one of the most common complications of hemorrhoids, which seriously affects the quality of life of patients. Medical hemorrhoid gel (MHG), a product mainly composed of herbal medicine, is widely used for treatment of PA clinically. This study aim to assess the alleviating effect and mechanism of MHG on PA based on rectal epidermis-spinal cord-brain axis using animal models.

Using the coefficient of determination to identify injury regions after stroke in pre-clinical FDG-PET images.

Background: In the analysis of brain fluorodeoxyglucose positron emission tomography (FDG-PET) images, intensity normalization is a necessary step to reduce inter-subject variability. However, the choice of the most appropriate normalization method in stroke studies remains unclear, as demonstrated by inconsistent findings in the literature.

Materials And Methods: Here, we propose a regression- and single-subject-based model for analyzing FDG-PET images without intensity normalization.

Effects of electroacupuncture on microglia phenotype and epigenetic modulation of C/EBPβ in SAMP8 mice.

Background: Alzheimer's disease (AD), an age-progressive neurodegenerative disease, is featured by a relentless deterioration of cognitive abilities. In parallel with the hypotheses of Aβ and tau, microglia-mediated neuroinflammation is a core pathological hallmark of AD. Promoting the transition of microglia from M1 to M2 phenotype and inhibition of neuroinflammatory response provide new insights into the treatment of AD.

Characterization of a novel cold-active β-Xylosidase from Parabacteroides distasonis and its synergistic hydrolysis of beechwood xylan.

Although β-xylosidases have broad applications in fields such as food and medicine, there is limited research on cold-active β-xylosidases. This study cloned a novel cold-active β-xylosidase XYL13 from Parabacteroides distasonis. The purified XYL13 exhibited the highest activity at 40 °C, with 42 % and 25 % of its maximum activity at 4 °C and 0 °C, respectively.

Integrated Studies on Male Reproductive Toxicity of Decabromodiphenyl Ethane in Zebrafish Spermatozoa , Male Zebrafish , and GC-1 Cells .

Background: Legacy brominated flame retardants have been recognized as risky factors leading to declined sperm quality. The widespread utilization of decabromodiphenyl ethane (DBDPE) as a replacement for decabromodiphenyl ether has given rise to considerable concern over its potential risks to reproductive health.

Objectives: The objectives were to quickly determine whether DBDPE affects sperm quality upon exposure, to reveal the reproductive outcomes and underlying molecular mechanisms using an zebrafish model exposed to DBDPE, and to validate the potential impact on DNA damage and energy metabolism balance .

Spatial and Temporal Variation of Precipitation Drives the Genome Size Variation in in Chinese Mainland.

Genome size is an adaptive trait, and its variations influence the organismal phenotype and fitness. In this study, we propose a hypothesis linking variations in genome size within to ecological factors. To test this hypothesis, we employed flow cytometry to estimate genome size in seven species from Chinese mainland.

A machine learning prediction model for Cardiac Amyloidosis using routine blood tests in patients with left ventricular hypertrophy.

Current approaches for cardiac amyloidosis (CA) identification are time-consuming, labor-intensive, and present challenges in sensitivity and accuracy, leading to limited treatment efficacy and poor prognosis for patients. In this retrospective study, we aimed to leverage machine learning (ML) to create a diagnostic model for CA using data from routine blood tests. Our dataset included 6,563 patients with left ventricular hypertrophy, 261 of whom had been diagnosed with CA.

Structural characteristics and potential antidepressant mechanism of a water-insoluble β-1,3-glucan from an edible fungus Wolfiporia cocos.

A water-insoluble β-1,3-glucan (Wβ) with a molecular weight of 8.12 × 10 Da was extracted from an edible fungus Wolfiporia cocos. Its backbone was composed of 1,3-β-linked Glcp branched at the C-2, C-4, and C-6 positions, connecting more 1,3-β-linked Glcp with a triple helical structure.

Ethyl acetate extract of Artemisia argyi improves the resistance of cotton to Verticillium dahliae by activating the immune response.

Verticillium wilt, a significant pathogen affecting cotton, has historically been challenging to control, posing a substantial threat to the sustainable development of the cotton industry. This study demonstrates that resistance to Verticillium dahliae in cotton can be enhanced by treating the roots with an ethyl acetate extract (EAAA) extracted from Artemisia argyi. The mechanisms by which EAAA activates immunity in cotton were elucidated by examining the expression levels of resistance genes post-treatment, evaluating salicylic acid (SA) and jasmonic acid (JA) levels, analyzing transcriptome data, and employing virus-induced gene silencing (VIGS) technology.

A retrospective study of recombinant human growth hormone in the treatment of school-aged pediatric patients with idiopathic short stature.

The present study aims to investigate the clinical efficacy of recombinant human growth hormone (r-hGH) in the treatment of school-age pediatric patients with idiopathic short stature (ISS). Pediatric patients who were diagnosed with ISS and treated with r-hGH at our hospital were enrolled as research subjects. The main outcome indicators included the serum level of insulin-like growth factor-1, insulin-like growth factor binding protein-3, baseline height standard deviation scores, and posttreatment height standard deviation scores and retrospective analysis was performed.

Leveraging AI technology for distinguishing Eucommiae Cortex processing levels and evaluating anti-fatigue potential.

Eucommiae Cortex (ECO) is a traditional medicinal and edible plant endemic to China, highly prized for its numerous health benefits. It typically undergoes special processing before application. The efficacy of ECO is influenced by processing techniques, necessitating the assurance of stability and consistency in its effects.

Artemisia argyi leaf powder improves soil properties and recruits Sphingobium bacteria to promote the growth and yield of Pinellia ternata.

Recent research has reported the strong herbicidal activity of Artemisia argyi leaf powder (AALP), indicating its high potential for use as an environmentally friendly weed management solution for ecological agriculture. However, AALP's impacts on soil physicochemical properties and microbial communities have remained uninvestigated. This study explores these effects through pot experiments assessing the AALP's efficacy in weed suppression and its ability to promote the growth of Pinellia ternata, a plant utilized in traditional Chinese medicine.

AnMei decoction ameliorates cognitive impairment in rats with chronic sleep deprivation by mitigating hippocampal neuroinflammation and restoring synaptic architecture.

Significance Of Ethnopharmacology: AnMei Decoction (AMD) is a renowned herbal prescription that has been widely demonstrated to have positive therapeutic effects on sleep disorders, depression, and cognitive impairments. However, the molecular mechanisms underlying AMD's resistance to sleep deprivation-induced cognitive impairment remain to be further investigated.

Research Objective: To clarify whether AMD may alleviate neuroinflammation by inhibiting NLRP3/Caspase1 signaling pathway and repair neuronal damage by regulating BDNF/TrkB pathway, thereby improving cognitive dysfunction in rats with chronic sleep deprivation.

A Rapid Colorimetric and Fluorescent Assay of in Food by Loop-Mediated Isothermal Amplification.

is a foodborne mycete that can induce recurrent pneumonia, but the current detection methods have insufficient sensitivity and rapidity. Here, we aim to develop an efficient and sensitive loop-mediated isothermal amplification (LAMP) primer set for detection. First, we designed a novel set of LAMP primers by targeting the gene.

Dual group-based trajectories of physical activity and cognitive function in aged over 55: a nationally representative cohort study.

Background: As individuals age, they commonly experience reduced physical activity and cognitive decline. While evidence, there is limited trajectory research on their concurrent progression and interrelation in individuals over 55 years old.

Methods: The data was collected from 5,765 individuals aged over 55 years who participated in the China Health and Retirement Longitudinal Study (CHARLS) between 2011 and 2020.

NLRP12/C1qA positive feedback in tumor-associated macrophages regulates immunosuppression through LILRB4/NF-κB pathway in lung adenocarcinoma.

The anti-tumor immune response is greatly hindered by the protumor polarization of tumor-associated macrophages (TAMs). Cancer-related inflammation plays a central role in TAMs protumor polarization. Our study explored the unique positive feedback loop between inflammasome and complement in TAMs.

Relationship of thyroid parameters with chronic inflammation in patients with euthyroid type 2 diabetes.

Aims: We evaluated the association of multiple thyroid parameters with the levels interleukin 6 (IL6) and interleukin 8 (IL8) in patients with euthyroid type 2 diabetes (T2D).

Methods: A total of 166 adults with euthyroid T2D were examined. Serum IL6, IL8, triiodothyronine (FT3), free thyroxin (FT4), thyroid-stimulating hormone (TSH), five thyroid sensitivity indexes (FT3/FT4, TSH index [TSHI], thyrotroph T4 resistance index [TT4RI], thyroid feedback quantile-based index by FT3 [TFQI], and TFQI), and biochemical parameters were determined.

SlSPA3 regulates the nuclear abundance of SlUVR8 in tomato.

Tomato (Solanum lycopersicum L.) is an important model plant species in photomorphogenesis research. Ultraviolet B (UV-B) induces the dissociation of homodimers of the photoreceptor UV RESISTANCE LOCUS8 (UVR8) into monomers, which translocate into the nucleus.

Glabridin inhibits proliferation and migration in hepatocellular carcinoma by regulating multi-targets.

Ethnopharmacological Relevance: Glycyrrhiza uralensis Fisch. (GC) is widely utilized in traditional Chinese medicine (TCM) for its properties in Qi tonification, heat clearing, and detoxification. Within TCM theory, Qi is also implicated in tumor development.

Isoscopoletin inhibits hepatocellular carcinoma cell proliferation via regulating glycolysis-related proteins.

Objective: Isoscopoletin is one of the primary metabolites of natural product scoparone, which was reported to against tumor proliferation. The aim of this study was to explore the mechanism of isoscopoletin against hepatocellular carcinoma (HCC).

Methods: Transcriptomics was used to reveal the possible pathways of isoscopoletin against HCC in vitro.