[The General medical journal of China].

The , started by Hou Yuwen in Beijing in 1923, was renamed the in 1933. This journal influenced the medical field in terms of improving medical development, and in particular, playing an important role in spreading western medicine in China in the 1920s and 1930s. The journal was operated and issued in Beijing, Shenyang, and Changchun successively, recording important medical activities and medical experiences at that time, presenting the historical events of multi-dimensional interaction and exchange between medicine and society, medicine and culture, and medicine and politics.

Cost-effectiveness of lung cancer screening combined with nurse-led smoking cessation intervention: A population-based microsimulation study.

Background: The appropriate screening inclusion criteria of low-dose computed tomography screening for lung cancer in Chinese population remains unclear and the effect of combining screening with nurse-led smoking cessation intervention is poorly understood as well.

Objective: We compared the benefits and costs of lung cancer screening with and without nurse-led smoking cessation intervention in different inclusion criteria to help select optimal screening strategies.

Methods: Different screening strategies were set based on diverse starting ages, smoking pack-year and whether nurse-led smoking cessation intervention was applied.

Corrigendum to "Ethanol Extract from Ampelopsis sinica Root Exerts Anti-Hepatitis B Virus Activity via Inhibition of p53 Pathway In Vitro".

[This corrects the article DOI: 10.1093/ecam/neq011.].

The gastrointestinal irritation of polygala saponins and its potential mechanism in vitro and in vivo.

Processing alters the pharmacological activity and reduces the gastrointestinal toxicity of the polygalae. To investigate the effect of processing, different glycosyl substituent products were tested. Hypnotic and subhypnotic doses of pentobarbital-induced sleep tests on mice were used to evaluate the sedative activity of polygala saponins with different glycosyl substituents; isolated gut motility experiment was employed to study excitatory effects of different polygala saponins; the gastrointestinal irritation effects of different polygala saponins were compared by measuring the levels of gastric PGE2 and intestinal TNF-α on mice.

Comparison of cytotoxic activities of extracts from Selaginella species.

Background: Selaginella species are resurrection plants, which are known, possess various molecular bioactivities depending on species, but only a few species have been detailed observe in the advanced research.

Objective: The objective of the following study is to compare the chemical profiles of different species of Selaginella and to investigate cytotoxicity and induction of apoptosis activities of some species of Selaginella.

Materials And Methods: The high-performance liquid chromatography (HPLC) method was developed for chemical analysis.

Activity of antibacterial, antiviral, anti-inflammatory in compounds andrographolide salt.

Andrographolide sulfonic acid sodium salt (ASS) was synthesized to increase the the solubility of Andrographolide in aqueous solution. We have studied its pharmacological effect of antibiosis, anti-inflammatory and immunoregulation. Cylinder-plate method was used to study ASS׳s in vitro antibacterial activity, and its protection for mice infected by Staphylococcus aureus and Shigella dysenteriae.

Study on traditional Chinese medicine theory of lung being connected with large intestine.

Objective: The theory of lung being connected with large intestine, which is a major topic in Traditional Chinese Medicine (TCM), has guided clinical practice for thousands of years in China.

Methods: In this study, we analyzed the history, main contents, clinical application, and material basis of the theory, to attempt to improve the potential clinical significance of "lung being connected with large intestine" in China.

Results: The lung being connected with large intestine was first described in "Huang Di Nei Jing", and formed one of the basic theories of TCM.

Inhibition of 5-HT(3) receptors-activated currents by cannabinoids in rat trigeminal ganglion neurons.

This study investigated the modulatory effect of synthetic cannabinoids WIN55,212-2 on 5-HT(3) receptor-activated currents (I(5-HT3)) in cultured rat trigeminal ganglion (TG) neurons using whole-cell patch clamp technique. The results showed that: (1) The majority of examined neurons (78.70%) were sensitive to 5-HT (3-300 μmol/L).

[Analysis of genetic diversity of gemplasm of Pinellia ternata based on SRAP].

Objective: To assess population genetic diversity of Pinellia ternata with different phenotype and from different habitat by sequence-related amplified polymorphism (SRAP) technique.

Method: Fourteen appropriate primer pairs were selected out from a total of 80 pairs for SRAP PCR amplification. A Jaccard's genetic similarity matrix and a dendrogram were established using SPSS 16.

[DNA barcoding in medicinal plants Caprifoliaceae].

Objective: To determine the candidate sequences which can be used as DNA barcode to identify species in Caprifoliaceae family by screening out from four different DNA fragments sequences.

Method: PCR amplification, sequencing efficiency, differential intra- and interspecific divergences, the DNA barcoding gap and identification efficiency were used to evaluate these loci.

Result: The ITS2 was used as a candidate sequence of DNA barcode to identify the species in Caprifoliaceae family, whose rate of success in identification in genera level was 100% and in species 96.

Anti-influenza-virus activity of total alkaloids from Commelina communis L.

The antiviral activity of total alkaloids from Commelina communis L. (TAC) against influenza virus A/PR/8/34 (H1N1) was investigated in vitro and in vivo. TAC exhibited an inhibitory action on the growth of influenza virus in Madin-Darby canine kidney cells when added before or after viral infection.

[Influence of electroacupuncture of "Shuigou" (GV 26) on hippocampal CGRP and NPY contents in cerebral ischemia rats].

Objective: To observe the effect of electroacupuncture (EA) on the contents of hippocampal calcitonin gene-related peptide (CGRP) and neuropeptide Y (NPY) in cerebral ischemia (CI) rats.

Methods: Forty SD rats were randomly divided into normal control, sham operation (sham), model and EA groups with 10 cases in each. CI model was duplicated by middle cerebral artery occlusion.

[Studies on chemical constituents of Dendranthema indicum var. aromaticum].

Objective: To study chemical constituents of Dendranthema indicum var. aromaticum.

Methods: The constituents were separated and purified by column chromatography with silica gel and Semi-preparative HPLC.

Bioactive compounds of inhibiting xanthine oxidase from Selaginella labordei.

Four flavone compounds were isolated from the effective fractions inhibiting xanthine oxidase (XOD) of the medicinal plant Selaginella labordei with anti-virus activity, and the structures were elucidated as 4'-methylether robustaflavone (1), robustaflavone (2), eriodictyol (3) and amentoflavone (4). The 50% inhibitory concentration (IC(50)) of the three compounds of inhibiting XOD were 61.0, 0.

[Analysis of the chemical constituents of essential oil from Magnolia biondii by GC-MS].

Objective: The chemical components of essential oil from Magnolia biondii were analyzed by GC-MS.

Methods: Essential oil was extracted by steam distillation (SD). The chemical components of essential oil were analyzed by GC-MS.

Analysis on the features of preventive treatment with acupuncture and moxibustion.

Objective: To analyze the features of preventive treatment with acupuncture and moxibustion to provide guidance for practice.

Methods: Induction and comparison methods were adopted to summarize the features of preventive treatment from the literatures of different ages.

Results: Preventive treatment has the distinctive features: (1) strengthening and protecting the vital-qi is paid much attention to; (2) the intervention time is well grasped; (3) tonification points are importantly used; (4) the action of moxibustion is stressed on; and (5) the change of disease is judged.

[Therapeutic effects of multi-stage manipulative reduction and splint fixation in 22 patients with tibia shaft fracture].

Objective: To study the method and effects of multi-stage manipulative reduction and homemade splint fixation in the treatment of tibia shaft fractures.

Methods: Twenty-two patients (16 male and 6 female) were involved the retrospective study. The average age was 33 years (from 6 to 54 years).

Determination of organochlorine pesticides and their metabolites in radish after headspace solid-phase microextraction using calix[4]arene fiber.

A novel laboratory-made sol-gel calix[4]arene/hydroxy-terminated silicone oil coated fiber has been applied for headspace solid-phase microextraction (HS-SPME) combined with gas chromatography (GC) with electron capture detection (ECD) to determine 12 organochlorine pesticides (OCPs) and their metabolites in radish sample. The analytes in the study consisted of alpha-, beta-, gamma- and delta-hexachlorocyclohexane (BHC), 1,1,1-trichloro-2-(2-chlorophenyl)-2-(4-chlorophenyl)ethane (o,p'-DDT), 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (p,p'-DDT), 2,4-dichlorobenzophenone (o,p'-DBP), 4,4-dichlorobenzophenone (p,p'-DBP), 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (p,p'-DDE), bis(4-chlorophenyl)methane (p,p'-DDM), 1,1-dichloro-2,2-bis(4-chlorophenyl)ethane (p,p'-DDD) and endrin. The following parameters were adjusted to optimize HS-SPME in order to obtain the maximum sensitivity: extraction temperature, extraction time, the addition of salt, desorption temperature and time.

[Phenols in seedling cortex of Magnolia officinalis from Enshi].

Objective: To study the phenols in Cortex of Magnolia officinalis of biernial seedings produced in Enshi, Hubei.

Methods: The content of magnolol and honokiol in cortexes were determined by HPLC. The chromatograms of 10 samples seedling cortexes were recorded and compared.

[Effects of Salvia miltiorrhiza on TGF-beta1, ROS and PAI-1 induced by angiotensin II in renal mesangial cells].

Objective: To investigate the effect of Salvia miltiorrhiza on glomerulosclerosis induced by Ang II.

Method: Rat mesangial cells were exposed to 100 nmol L(-1) Ang II. Meanwhile, we added S.

[Studies on the HPLC fingerprint spectrum of flavonoids in Gynostemma pentaphyllum].

Objective: To establish the HPLC fingerprint spectrum of flavonoids from Gynostemma pentaphyllum.

Methods: The HPLC conditions with TURNER Kromasil C18 column (250 x 4. 6 mm, 5 microm) was used, the mobile phase consisted of 0.

[Analysis of lipid compounds of high-yielded rhizoma pinelliae growing in the west of Hubei province by gas chromatography-mass spectrometry].

Objective: To investigate lipid components of high-yielded Pinellia ternata rhizomes growing in the west of Hubei province.

Methods: To determine the lipid chemical components in Pinellia ternata rhizomes with GC-MS method and NIST atlas.

Results: Ten components have been found: palmitic acid (I), 9,12-octadecadienoic acid (II), pyrrolidine,1-(1-oxo-7,10-hexadecadienyl) (III), alpha-monpalmitin (IV), 1,3,12-nonadecatriene (V), campesterol (VI), stigmasta-5,22-dien-3-ol (VII), beta-sitosterol (VIII), stigmasta-5,24-dien-3-ol (IX), cycloartenol (X).

[Determination of three glycosides from herbs of Swertia punicea by RP-HPLC].

Objective: To develop a RP-HPLC method for determination of three glycosides in Swertia punicea.

Method: Chromatographic column: Alltimal C18 (4.6 mm x 250 mm, 5 microm).