[Influence on electroacupuncture at "Qiangzhuang" acupoints for neuro-immune regulation of sub-acute aged rats].

Objective: To explore the underlying mechanism of electroacupuncture for anti-aging.

Methods: Forty Sprague-Dawley rats (female and male take one half for each), 3-month old, were divided into a control group, a model group, a routine electroacupuncture group (current intensity, 1 mA) and a strong electroacupuncture group (current intensity, 4.5 mA), 10 rats in each group.

[Studies on triterpenoid saponins in the rhizome of Anemone flaccida].

Objective: To study the triterpenoid saponins in the rhizome of Anemone flaccida.

Methods: The constituents were separated with various chromatographic techniques and their structures were elucidated by means of physicochemical properties and the analysis of their spectral datas.

Results: Five compounds were isolated and identified as 3-O-beta-D-glucuronypyranosyl-oleanolic acid-28-O-alpha-L-rhamnopyranosyl (1 --> 4)-beta-D-glucopyranosyl(1 --> 6)-beta-D-glucopyra noside (1), 3-O-beta-D-glucuronypyranosyl-oleanolic acid-28-O-beta-D-glucopyranosyl (1 --> 6)-beta-D-glucopyranoside (2), 3-O-alpha-L-rhamnopyranosy (1 --> 2)-beta-D-glucopyranosyl-oleanolic acid-28-O-alpha-L-rhamnopyranosyl (1 --> 4)-beta-D-glucopyranosyl (1 --> 6)-beta-D-glucopyranoside (3), 3-O-alpha-L-rhamnopyranosyl (1 --> 2)-alpha-L-arabinopyrano-syl-oleanolic acid-28-O-alpha-L-rhamnopyranosyl (1 -->4)-beta-D-glucopyranosyl (1 --> 6)-beta-D-glucopyranoside (4), 3-O-alpha-L-rhamnopyranosyl (1 --> 2)-beta-D-xylopyranosyl-oleanolic acid-28-O-alpha-L-rhamnopyranosyl (1 --> 4)-beta-D-glucopyranosyl (1 --> 6)-beta-D-glucopyranoside (5).

[Effects of different acupuncture treatment on mean blood flow velocity of middle cerebral artery on the affected side and rehabilitation of hemiparalysis caused by cerebral infarction].

Objective: To observe the effects of two different acupuncture treatment on cerebral blood flow velocity and early rehabilitation of hemiparalysis caused by cerebral infarction.

Methods: Eighty patients were randomly divided into an alternate acupuncture group (n = 40) and a routine acupuncture group (n = 40). Both of the groups were treated with routine neurology medicine and application of good limb position combined with acupuncture.

[HPLC fingerprint of the antiarrhythmic fraction of Valeriana officinalis].

Objective: To establish HPLC fingerprints of the Antiarrhythmic fraction of Valeriana officinalis.

Methods: Agilent C18 (250 mm x 4.6 mm, 5 microm) column was used and the acetonitrile-water was chosen as the mobile phase in a gradient mode.

[Pharmacognostic identification of Centipeda minima].

Objective: To conduct pharmacognostic identification of Centipeda minima.

Methods: To conduct characteristic identification, micro-identification and TLC identification of Centipeda minima.

Results: We established a systematic pharmacognostic identification method including characteristic identification, micro-identification and TLC identification.

Triterpenoid saponins from Anemone flaccida induce apoptosis activity in HeLa cells.

Five triterpenoid saponins were isolated from Anemone flaccida Fr. Schmidt. Their structures were identified as glycoside St-I4a (1), glycoside St-J (2), anhuienoside E (3), hederasaponin B (4), and flaccidoside II (5).

[Clinical observation on acupuncture combined with medication for treatment of continuing unovulation infertility].

Objective: To explore the therapeutic effect of acupuncture combined with medication on continuing unovulation infertility.

Methods: Fifty cases of continuing unovulation infertility were randomly divided into an observation group and a control group, 25 cases in each group. The observation group was treated with Chinese herbal decoction plus acupuncture.

[Clinical observation on acupoint-injection for treatment of lumbago-leg pain induced by primary osteoporosis].

Objective: To compare therapeutic effects of acupoint-injection and Chinese herbs for treatment of lumbago-leg pain induced by primary osteoporosis.

Methods: Ninety cases of lumbago-leg pain induced by primary osteoporosis were randomly divided into a Jiaji point group, a reinforcing kidney and strengthening spleen point group and a medication group, 30 cases in each group. For the Jiaji point group, bilateral Jiaji points T11, L1, L3, L5 and S2 were selected; for the reinforcing kidney and strengthening spleen point group, Pishu (BL 20), Shenshu (BL 23), Taixi (KI 3), Taibai (SP 3), Taichong (LR 3), Sanyinjiao (SP 6), Xuehai (SP 10) were selected and the medication group were treated with oral administration of Qianggu Capsules.

[Studies on HPLC-ELSD fingerprint of the Coleus forskohlii introduced in Tongcheng].

Objective: To establish HPLC-ELSD fingerprint of Coleus forskohlii.

Methods: Chromatographic fingerprint of Coleus forskohlii was investigated by HPLC-ELSD and gradient elution mode was applied to chromatographic separation.

Results: The HPLC-ELSD fingerprint of Coleus forskohlii was established preliminarily.

[Study on dual-directional regulation induced by acupuncture at different acupoints in the rat with motility abnormality of the stomach].

Objective: To probe into the bidirectional regulation of acupuncture in the rat with motility abnormality of the stomach and the nerve mechanism.

Methods: Adopting electrophysiological methods, the extracellular discharge signals of neuronic action in NTS were recorded with glass microelectrodes. The effects of acupuncture at "Zusanli" (ST 36), "Neiguan" (PC 6), "Zhongwan" (CV 12), "Qihai" (CV 6) on discharges of NTS neurons and intra-gastric pressure were observed in the rat at normal physiological state, and increase or reduction of gastric motility.

[Studies on sodium dodecyl sulphate-polyacrylamide gel electrophoresis atlas of protein in precious animal TCM].

Objective: To offer scientific basis of distinguish p recious animal TCM.

Method: Molecular weights of the proteins were measured by modified sodium dodecyl sulphate-polyaerylamide gel eleetrophoresis (SDS-PAGE).

Results: The bands were always quite distinct.

[Studies on HPLC-FPS of the saponins from Semen Ziziphi Spinosae].

Objective: To establish a method of HPLC-fingerprint spectrum (HPLC-FPS) for the active part in Semen Ziziphi Spinosae (SZS) in order to control the quality of SZS from different places.

Methods: The gradient elution mode was applied in chromatographic separation, and data were analysed by" Similarity Evaluation software" to compare the HPLC-FPS of SZS from different places.

Results: The conditions for HPLC analysis of SZS were established and the FPS of samples from different habitats showed some differences.

[Effects of electroacupuncture on behaviors and dopaminergic neurons in the rat of Parkinson's disease].

Objective: To explore the possibility of electroacupuncture (EA) for prevention of the injury of dopaminergic neurons in the substantia nigra of the rat with Parkinson's disease.

Methods: Wistar rats were randomly divided into a normal group, a model group, a sham-operation group and an EA group. 6-OH-DA was injected into right substantia nigra of the midbrain to made Parkinson's disease rat model with single side substantia nigra injury, and TH/TUNEL method and rotation behavior observation method were used to observe changes of rotation behavior and apoptosis of dopaminergic neurons in the substantia nigra after EA at "Taichong" (LR 3) and "Fengfu" (GV 16) for 3 d, 7 d and 14 d.

[Effect and mechanism of shenshuai mixture (SM) in promoting repair of kidney in acute renal failure rats].

Objective: To dynamically observe the effect of Shenshuai Mixture (SM) on repair of kidney in acute renal failure (ARF) rats.

Methods: Male SD rats were divided into 4 groups randomly, the normal group, the SM group, the verapamil group and the model control group. Except those in the normal group were treated with normal saline without modeling, all remaining rats, after being made into ARF model by intra-muscular injection of glycerin, were treated with SM, verapamil and normal saline respectively via gastrogavage.

[Pharmacognostic studies on Begonia sinensis rhizome].

Objective: To establish the characteristic method of rude medicinal material of rhizome of Begonia sinensis.

Method: The characteristic features of the rhizome were studies by morphological and microscopic observation.

Results: The description and transverse features of transverse section of the rhizome were described, and in the powder the microscopic features such as cluster needle crystals, tentacle, ring-thread vessel were found.

[New explaination of mechanisms of indications of Suliao (GV 25) and Shuigou (GV 26)].

Objective: To newly explain mechanisms of indications of Suliao (GV 25) and Shuigou (GV 26).

Methods: Analyze mechanisms of indications of Suliao (GV 25) and Shuigou (GV 26) in TCM "viscera-state doctrine" as the center and in combination with anatomy of western medicine.

Results: Not only put forward the corresponding relation ("re-preparation" of external forms) of the five sense organs with five solid organs, and also use this theory to explain the reasons why Suliao (GV 25), Shuigou (GV 26) and round some extra-ordinary points (Bijian, Bizhonggou) have same indications.

[The role of endogenous opioid peptides in paraventricular nucleus of hypothalamus in the antagonism to myocardial ischemia by electroacupuncture at acupoint "Neiguan" (PC 6)].

Objective: To explore the mechanism of electroacupuncture at "Neiguan" (PC 6) antagonizing myocardial ischemia injury.

Methods: Forty rabbits were randomly divided into 4 groups, model control group, naloxone control group, electroacupuncture plus cerebral spinal fluid group, and electroacupuncture plus naloxone group, 10 rabbits in each group. The effects of electroacupuncture at "Neiguan" (PC 6) after injection of naloxone into the paraventricular nucleus (PVN) of the hypothalamus on myocardial ultrastructure, II lead ECG and transmembrane potentials of the ischemic myocardial cell in the rabbit of myocardial ischemia were investigated.

[Discussion on several commonly-seen problems in teaching of the Acupuncture and Moxibustion Science].

The arrange of teaching material, finger force in needling, rapidly needle-inserting method, heavy thrusting and light lifting the needle, light thrusting and heavy lifting the needle, keeping qi, needling depth and other aspects are discussed in combination with experiences in teaching of The Acupuncture and Moxibustion Science and clinical practice of many years, so as to help to grasp relative knowledge for beginners in learning Acupuncture and Moxibustion Science.

[Study on extracting oil of radix Angelica sinensis with supercritical CO2].

Objective: To study the extraction of angelica oil by SFE-CO2 (CO2 Supercritical Fluid Extraction).

Methods: Investigating the influence of the granule size of medical material, extraction temperature, extraction pressure, extraction time, releasing and separating condition on the extraction of angelica oil by the orthogonal design and other methods.

Results: The optimum extraction is as follows: the granule size of medical material, 40 mu; extraction temperature, 40 degrees C; extraction pressure, 25Mpa; extraction time, 2 hours; flow of CO2, 20 L/h; pressure of separation pot I, 8Mpa; temperature of separation pot I, 50 degrees C; pressure of separation pot II, 6Mpa; temperature of separation pot II, 50 degrees C.

[Clinical study on treatment of exogenous infection caused fever by infantile umbilical paste].

Objective: To observe the effect of infantile umbilical paste (IUP) in abating infantile fever.

Methods: Two hundred and twenty cases were randomly divided into two groups, the 120 cases in Group A were treated with IUP and the 100 cases in Group B treated with compound Aspirin (APC). The effect in abating fever and side-effects were observed.

[Discussion of leigong 17 processing methods].

"Seventeen Methods" plays an important role in the field of processing medicinal herbs, and also makes a notable impact on modern ages. However, some problems among it are still existed. According to the origin of "17 Methods" and interrelating records, a simple description about its objections was given.

[Separation and identification of chemical compositions from cultivated Belamcanda chinensis].

In this paper, two compounds, isoflavone III and IV are isolated. On the bases of physical, chemical and spectral evidences, the structure of composition III is tectoridin, and composition IV is tectoriginin.

Clinical research on acute hemoptysis treated with kexue ning.

The efficacy of Kexue Ning (stopping hemoptysis) in the treatment of acute hemoptysis, and its comparison with that of the control group (treated with western medicine) are reported in this paper. The results showed that both the time for cessation of hemoptysis and the cure rate were better in the therapeutic group than in the control group. In addition, marked improvement in blood rheology after treatment was also found in the therapeutic group, but not in the control group.

[Studies on function of vegetative nervous system in cardiopathic patients with heart-qi deficiency syndromes and heart-yin deficiency syndromes].

To explore the changes of the function of vegetative nervous system in the patients with the syndromes of Heart-Qi Deficiency (HQD) and Heart-Yin Deficiency (HYD), 53 cases of heart diseases were divided into HQD and HYD, the duration between heart beats, differences of blood pressure in the lying and standing position, and urine-catecholamine/24 hrs was determined separately, and compared with control group. The results showed that the duration between heart beats and heart rate in the lying position in those patients of HQD didn't manifest significant difference with control group. But in 78.

[A study on pulmonary artery compliance and hemodynamics in patients with lung-distention].

A simultaneously comparative study on pulmonary artery compliance (Cpa) and pulmonary artery mean pressure (PAMP) in 89 patients with Lung-Distention in TCM by impedance pulmonary rheogram, tricuspid valve echocardiogram and right heart catheterization. Then according to Syndrome Differentiation of TCM, Lung-Distention was sub-divided into four groups. The results showed that PAMP were gradually increased and Cpa decreased in these groups.