Analysis of prognosis on patients with severe viral hepatitis using the model for end-stage liver disease.

Aim: To study the practical use of the model for end-stage liver disease (MELD) on clinic and assess its validity by the concordance (C)-statistic in predicting the prognosis of the patient with severe viral hepatitis.

Methods: One hundred and twenty-one patients were divided into plasma exchange group and non-plasma exchange group, and were graded with MELD formula. The death rate was observed within 3 mo.

[Relationship between the pathologic changes of human embryo fibroblast cells and expression of late mRNA after human cytomegalovirus infection in vitro].

Objective: To study the relationship between late mRNA and the cytopathic effect(CPE) and ultrastructural features after human cytomegalovirus (HCMV) infection in vitro.

Methods: Human embryo fibroblast cells(HEL) were infected with HCMV AD169 strain. The expression of the HCMV late mRNA was measured by semiquantitative RT-PCR, the cytopathic effect (CPE) and the cell ultrastructure were observed by means of light microscopy and electron microscopy, respectively.

[PCR site-directed mutagenesis of long QT syndrome KCNQ1 gene in vitro].

To study PCR site-directed mutagenesis of long QT syndrome KCNQ1 gene in vitro. The site-directed mutagenesis of LQTS gene KCNQ1 was made by PCR. Two sets of primers were designed according to the sequence of KCNQ1 cDNA, and mismatch was introduced into primers.

[Relationship between estradiol and the mitogenic activated protein kinase signal transduction pathway].

Objective: To discuss the relationship between estradiol and the mitogenic activated protein kinase signal transduction pathway and the expression of the MAPK in the MCF-7 breast cancer cell-line.

Methods: Epithelial growth factor (EGF) and different concentration of estradiol to induce the expression of phosphospecific ERK1/2 (pERK1/2) in MCF-7 cell line was used and the expression of pERK1/2 with western-blotting was detected. Then antiestrogen ICI 182780 and MAPK inhibitor PD98059 to inhibit the expression of pERK1/2 was used.

[The inhibitory effects of angiostatin on retinal neovascularization induced by oxygen].

Objective: To observe the inhibitory effects of angiostatin on microvascular endothelial cells of mouse retina and test the efficacy of native angiostatin in suppressing experimental retinal neovascularization induced by oxygen.

Methods: Angiostatin was purified with L-lysine Sepharose 4B from human plasma. The primary microvascular endothelial cells from rat retina were cultured.

[Expression of the heat-shock protein 70 family polymorphism in A549 cell line exposed to benzo(a)pyrene].

Objective: To study the pattern of polymorphism expression of heat-shock protein 70 (HSP70) family in A549 cell line treated with different concentrations of benzo(a)pyrene (BaP) and its probable biological effect.

Method: Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) was used for the HSP70 expression analysis.

Results: 2D-PAGE showed that when A549 cells were exposed to different concentrations of BaP (0.

[Effects of pyrethroids on dopamine and its metabolites in nigrostriatum of male rats].

Objective: To investigate the effects of pyrethroids on nigrostriatal dopaminergic pathways in male rats and its mechanism.

Methods: Different doses of permethrin (PM, 200, 400 mg/kg) and deltamethrin (DM, 6.25, 12.

[Effects of pyrethroids on tyrosine hydroxylase in nigrostriatum of male rats].

Objective: To study the effects of pyrethroids on tyrosine hydroxylase in nigrostriatum of male rats.

Methods: Different doses of permethrin and deltamethrin in corn oil were administered to rats by gavage for ten days, then PLC-Fluorescence detection was used to analyzed the contents of dopamine (DA), DOPAC and HVA in the Substantial Nigra and Striatum. The activities of TH were also detected by HPLC-Fluorescence detection.

[Detection of the mRNA level of the subunits of amiloride-sensitive Na+ channel in human bronchial epithelium cells from patients with chronic obstructive pulmonary disease].

Objective: To investigate the role of epithelial amiloride-sensitive Na(+) channel (ENaC) in the pathogenesis of chronic obstructive pulmonary disease (COPD) and the effect of interleukin-4 (IL-4) and IL-6.

Methods: Human bronchial epithelial cells were isolated and cultured from 18 patients who undergone pneumonectomy in Tongji hospital. The patients were allocated into COPD group, smoking group and control group based on their lung functions and smoking history, with 6 patients in each group.

[Tumor necrosis factor related apoptosis-inducing ligand induces apoptosis and sensitizes resistant hepatic cancer cells to chemotherapy].

Objective: To investigate the effective of tumor necrosis factor related apoptosis-inducing ligand (TRAIL) combination with chemotherapeutic agent inducing apoptosis in resistant hepatic cancer cells.

Methods: HepG2 cells resistant to Adriamycin (HepG2/ADR) were induced stepwise. The effects of TRAIL in combination with ADM (0.

Eukaryotic expression vector of heme oxygenase-1 and its expression in endothelial cell.

Background: Heme oxygenase-1 (HO-1)-mediated cytoprotection may inhibit or postpone the formation of graft arteriosclerosis. The aim of this study was to construct eukaryotic expression vector of HO-1 gene and express recombinant HO-1 in human endothelial cells of the umbilical vein to provide an important clue for chronic rejection study.

Methods: HO-1 gene was amplified from rat spleen with reverse transcription-polymerase chain reaction, and then cloned into eukaryotic expression vector pcDNA3 by means of recombinant gene technology.

[Smoking, the level of DNA adducts and chronic obstructive pulmonary diseases].

Objective: To investigate the association between DNA adducts (DNA-BPDE) and smoking, and the association of DNA adducts with the development of chronic obstructive pulmonary disease (COPD).

Methods: DNA-BPDE were tested with competitive enzyme-linked immunosorbent assay (ELISA) in passive smoking rats. DNA-BPDE were also tested in 68 people including smokers and non-smokers, and in 88 patients with COPD and 87 healthy smoking control subjects.

Inhibition of NF-kappaB by mutant IkappaBalpha enhances TNF-alpha-induced apoptosis in HL-60 cells by controlling bcl-xL expression.

Background: The aim of this study was to explore whether the inhibition of nuclear factor-kappaB (NF-kappaB) activation by mutant IkappaBalpha (S32, 36-->A) can enhance TNF-alpha-induced apoptosis of leukemia cells and to investigate the possible mechanism.

Methods: The mutant IkappaBalpha gene was transfected into HL-60 cells by liposome-mediated techniques. G418 resistant clones stably expressing mutant IkappaBalpha were obtained by the limiting dilution method.

Inhibition of NOS2 ameliorates cardiac remodeling, improves heart function after myocardial infarction in rats.

Objective: Previous studies have shown increased expression of nitric oxide synthase 2 (NOS2) in rat heart several weeks after myocardial infarction (MI). The aim of this study was to examine the effect of chronic administration of S-methylisothiourea (SMT), a selective NOS2 inhibitor, commenced one week after MI on hemodynamic parameters and left ventricular (LV) remodeling in rats.

Methods: Rats with MI induced by left coronary ligation were given SMT (0.

[Study on the mechanism of oocyte aneuploidy formation by multicolor fluorescence in situ hybridization].

Objective: To investigate the mechanism of oocyte aneuploidy formation.

Methods: The unfertilized oocytes were fixed 1-3 days after oocyte retrieval. Multicolor fluorescence in situ hybridization (M-FISH) was performed according to the Vysis protocol to check the chromosome status in oocytes by using centromeric enumerator probes for chromosomes 16, 18 and locus-specific probes for chromosomes 13, 21 and 22.

[The experimental study of guinea pig cytomegalovirus infection in the kidney of the pup of guinea pig].

Objective: To study the relationship of guinea pig cytomegalovirus (GPCMV) infection with the outcome of pregnancy by the kidney of guinea pig (GP).

Methods: Twenty first-trimester gestation GPs were randomly selected, intraperitoneally inoculated with GPCMV. Then female GPs and the pups were killed within 24 h after delivery.

Carboxymethylated chitin reduces MMP-1 expression in rabbit ACLT osteoarthritic cartilage.

Objective: To examine the in vivo effects of carboxymethylated chitin (CMC), intra-articularly administered, on cartilage degradation and the level and distribution of cartilage matrix metalloproteinase-1 (MMP-1).

Methods: Osteoarthritis (OA) was induced in 20 rabbits by unilateral anterior cruciate ligament transection (ACLT). The experimental group, comprising 10 rabbits randomly selected, was given an intra-articular injection of 0.

[Expression of cyclooxygenase-2 in esophageal squamous cell carcinogenesis].

Objectives: To investigate the expression of cyclooxygenase-2 (cox-2) protein in normal squamous epithelium, squamous dysplasia and squamous cell carcinoma of the esophagus, and to elucidate the role of cox-2 in esophageal carcinogenesis.

Methods: Biopsy specimens of atypical esophageal dysplasia (n = 47) and surgical resection of squamous cell carcinoma (n = 86) were compared with normal esophageal specimens (n = 42) and the expression of cox-2 in those specimens was analyzed by immunohistochemistry and western blotting.

Results: A significant elevated cox-2 expression was shown in atypical esophageal squamous dysplasia and squamous cell carcinoma, as compared to that in normal esophageal squamous epithelium, with immunohistochemical stain scores of 2.

[Expression changes of tau protein and microtubule-associated proteins in testis intersitium of rats].

Objective: To examine the expression of the tau protein and microtubule-associated proteins in the testis interstitium of aged and young rats.

Methods: Sprague-Dawley male rats were divided into a young group(6 months, n = 10) and an aged group(28 months, n = 10). The two steps immunohistochemistry method with the antibody against tau protein and MAP alpha was performed on the testis tissues of the rats.

Inhibitory effect of methylation inhibitor 5-aza-2-deoxycytidine on bile duct cancer cell line in vivo and in vitro.

Background: Since the resection rate is low for bile duct cancer and the drugs used for chemotherapy are less effective, we studied the inhibitory effects of 5-aza-2-deoxycytidine (ZdCyd) on bile duct cancer cell line QBC939 in vivo and in vitro and its possibility in clinical treatment.

Methods: The survival and apoptosis rates of QBC939 after treatment with different dose of ZdCyd were detected by methyl thiazoy tetrazolium (MTT) and flow cytometry. The cooperative effect of ZdCyd with other chemotherapeutic drugs was also studied with MTT.

[Study on the morphological damage and cell apoptosis of nerve tissue in mice exposed to benzo[a]pyrene].

Objective: To study the toxicological effects of benzo[a]pyrene(BaP) on mammalian animal's nerve tissue.

Methods: 50 Kunming mice were divided into 5 groups at random, the exposed groups(3 dose level groups), the vehicle control group and standard control group. Every group got 10 mice.

Bladder cancer therapy using combined proliferating cell nuclear antigen antisense oligonucleotides and recombinant adenovirus p53.

Objective: To evaluate the antitumor efficacy of proliferating cell nuclear antigen antisense oligonucleotide (PCNA-ASO) in combination with recombinant adenovirus p53 (Ad-p53) against bladder cancer EJ and BIU-87 cells in vitro and in vivo.

Methods: Cells were transfected with Ad-p53 (100 MOI), and PCNA-ASO (1.6 micro mol/L) was then introduced into the cells using a cationic lipid (lipofectamine, 20 micro l/ml).

[Insulin signal transduction disorder and development of insulin resistance].

Insulin action is initiated through the binding to its cell-surface receptor, stiring up a series of signal transduction and eventually reach various effectors which produce various physiological effects. Insulin signal transduction is crucial to insulin action. Impairment of insulin signal transduction results in attenuation of insulin action and finally leads to insulin resistance.

Acute respiratory distress syndrome after liver transplantation: etiology, prevention and management.

Objective: To study the etiology, prevention and management of acute respiratory distress syndrome (ARDS) after liver transplantation.

Methods: The clinical data of 104 patients with end-stage liver diseases who had had liver transplantations were retrospectively reviewed.

Results: Seventeen patients (16.