The influence of perceived landscape value on tourists' behavioral intentions in historical and cultural blocks.

Historic cultural blocks are areas where a city's material cultural heritage and humanistic characteristics converge, showcasing the city's unique features and preserving rich and complete urban memories. Research on historic blocks primarily involves strategies related to protection, renewal, planning, and enhancement. However, there is a paucity of studies that explore the relationship between landscape value perception and tourist behavioral intentions from the perspective of recreation participants during the development and renewal of historic cultural blocks.

Extracellular vesicles promote the infection and pathogenicity of Japanese encephalitis virus.

Japanese encephalitis virus (JEV) is a neurotropic zoonotic pathogen that poses a serious threat to public health. Currently, there is no specific therapeutic agent available for JEV infection, primarily due to the complexity of its infection mechanism and pathogenesis. Extracellular vesicles (EVs) have been known to play an important role in viral infection, but their specific functions in JEV infection remain unknown.

Evaluating the potential of in alleviation of aluminium stress in .

Soil contamination with toxic heavy metals [such as aluminum (Al)] is becoming a serious global problem due to the rapid development of the social economy. Although plant growth-promoting rhizo-bacteria (PGPR) are the major protectants to alleviate metal toxicity, the study of these bacteria to ameliorate the toxic effects of Al is limited. Therefore, the present study was conducted to investigate the combined effects of different levels of (5 ppm and 10 ppm) of accession number of MT123456 on plant growth and biomass, photosynthetic pigments, gas exchange attributes, oxidative stress and response of antioxidant compounds (enzymatic and nonenzymatic), and their specific gene expression, sugars, nutritional status of the plant, organic acid exudation pattern and Al accumulation from the different parts of the plants, which was spiked with different levels of Al [0 µM (i.

Wheat2035: Integrating Pan-omics and Advanced Biotechnology for Future Wheat Design.

Wheat (Triticum aestivum L.) production is vital for global food security, providing energy and protein to millions of people worldwide. Recent advancements in wheat research have led to significant increases in production, fueled by technological and scientific innovation.

Deciphering the Coupling State-Dependent Transcription Termination in the Escherichia coli Galactose Operon.

The distance between the ribosome and the RNA polymerase active centers, known as the mRNA loop length, is crucial for transcription-translation coupling. Despite the existence of multiple expressomes with varying mRNA loop lengths, their in vivo roles remain largely unexplored. This study examines the mechanisms governing transcription termination in the Escherichia coli galactose operon, revealing a crucial role in the transcription and translation coupling state.

Antibacterial mechanism of vitamin K-mediated photodynamic inactivation against Pseudomonas fluorescens and its influences on blunt bream (Megalobrama amblycephala) quality.

Photodynamic inactivation (PDI) has emerged as a novel non-thermal process technology for inactivating microorganisms due to its low cost, safety, and efficiency. This study aimed to investigate the antimicrobial effect of VK-mediated PDI against Pseudomonas fluorescens (P. fluorescens) and to assess its impact on the quality of the blunt bream contaminated with P.

Prediction of individual total amino acids and free amino acids in Chinese Holstein cows milk using mid-infrared spectroscopy and their phenotypic variability.

Establishing a high-throughput detection technology for amino acid (AA) content in milk using mid-infrared (MIR) spectroscopy has profound implications for enhancing nutritional value of milk, identifying superior milk sources, producing specialty dairy products, and expanding Dairy Herd Improvement (DHI) metrics. The aim of this study was to evaluate the effectiveness of MIR spectroscopy in predicting the content of 15 individual total AA (TAAs) and 16 free AA (FAAs) in bovine milk as well as to investigate the major factors affecting the phenotypic variability of AA content. From March 2023 to March 2024, 513 milk samples were collected from 10 Holstein dairy farms in China and analyzed using Bentley spectrometers for MIR measurements.

Prediction of the likelihood of conception to first or second insemination in Chinese Holstein cows using milk Fourier transformed infrared spectroscopy.

Accurate identification of cows' likelihood of conception during the period from recent calving to the first artificial insemination (AI) will provide assistance in managing the fertility of dairy cows and contribute to the economic prosperity and sustainability of the farm. The purpose of this study was to use FTIR spectroscopy collected from recent calving to the first artificial insemination (AI) to predict the cow's likelihood of conception to first AI, first or second AI. This study specifically focused on the role of FTIR spectral and farm data collected at different time windows in improving the accuracy of model for predicting the cow's likelihood of conception to first AI, first or second AI.

Enhanced immunogenicity of a BoHV-1 gG-/tk- vaccine.

Bovine herpesvirus type 1 (BoHV-1) is a widespread respiratory infection that significantly impacts cattle health worldwide. To address this issue in China, we previously developed a novel double gene-deleted vaccine targeting gG and tk. In this study, we further evaluated the efficacy of this vaccine by challenging vaccinated cattle with a prevalent wild-type BoHV-1 strain and comparing its effectiveness against a commercially available inactivated BoHV-1 vaccine.

tRNA thiolation optimizes appressorium-mediated infection by enhancing codon-specific translation in Magnaporthe oryzae.

Thiolation, a post-transcriptional modification catalyzed by Uba4-Urm1-Ncs2/Ncs6 pathway in three specific transfer RNAs (tRNAs), is conserved from yeast to humans and plays an important role in enhancing codon-anticodon interaction and translation efficiency. Yet, except for affecting effector secretion, its roles in plant pathogenic fungi are not fully understood. Here, we used Magnaporthe oryzae as a model system to illustrate the vital role of s2U34 modification on the appressorium-mediated virulence.

Visualization of Hg Stress on Plant Health at the Subcellular Level Revealed by a Highly Sensitive Fluorescent Sensor.

The presence of Hg causes substantial stress to plants, adversely affecting growth and health by disrupting cell cycle divisions, photosynthesis, and ionic homeostasis. Accurate visualization of the spatiotemporal distribution of Hg in plant tissues is crucial for the management of Hg pollution; however, the related research is still at its early stage. Herein, a small-molecule amphiphilic fluorescent probe (termed ) was developed for the specific detection of Hg with a high sensitivity (~16 nM).

Embracing plant plasticity or robustness as a means of ensuring food security.

The dual challenges of global population explosion and environmental deterioration represent major hurdles for 21 Century agriculture culminating in an unprecedented demand for food security. In this Review, we revisit historical concepts of plasticity and canalization before integrating them with contemporary studies of genotype-environment interactions (G×E) that are currently being carried out at the genome-wide level. In doing so we address both fundamental questions regarding G×E and potential strategies to best secure yields in both current and future climate scenarios.

Chromosome-level genome assembly of the seasonally polyphenic scorpionfly (Panorpa liui).

Mecoptera is a small relict order of insects within the Holometabola. Panorpidae is the most speciose family in Mecoptera. They are also known as scorpion flies due to the enlarged and upward recurved male genital bulb.

First Report of Influenza D Virus in Dairy Cattle in Pakistan.

Influenza D virus (IDV) is a newly emerged zoonotic virus increasingly reported worldwide. Cattle are considered the main reservoir of IDV, although it was first isolated from pigs. IDV infects multiple animal species and contributes to the bovine respiratory disease complex (BRDC).

Molecular Characterization and Pathogenicity Analysis of Porcine Rotavirus A.

Porcine rotavirus A (RVA) is one of the major etiological agents of diarrhea in piglets and constitutes a significant threat to the swine industry. A molecular epidemiological investigation was conducted on 2422 diarrhea samples from Chinese pig farms to enhance our understanding of the molecular epidemiology and evolutionary diversity of RVA. The findings revealed an average RVA positivity rate of 42% (943/2422), and the study included data from 26 provinces, primarily in the eastern, southern and southwestern regions.

Evaluation of the Effect of the Live Vaccine (Strain 168) in Ningxiang Pigs.

[Background/Objectives] () is widespread in the global swine industry, leading to significant economic losses, and is particularly severe in native Chinese pig breeds. The Ningxiang pig, a well-known native breed in China, is susceptible to , exhibiting high morbidity and mortality rates. This study was designed to evaluate the clinical effectiveness of the live vaccine (strain 168).

Comparative Analysis of Ca/Cation Antiporter Gene Family in and Enhanced Calcium Stress Tolerance via Heterologous Expression of RrCAX1a in Tobacco.

, a calciphilic species native to the mountainous regions of Southwest China, is renowned for its high vitamin C and bioactive components, making it valuable for culinary and medicinal uses. This species exhibits remarkable tolerance to the high-calcium conditions typical of karst terrains. However, the underlying mechanisms of this calcium resilience remain unclear.

Polystyrene Nanomicroplastics Aggravate Ammonia-Induced Neurotoxic Effects in Zebrafish Embryos.

The highly hazardous chemical ammonia has been proven to be absorbed by nanoparticles, thereby exerting highly toxic effects on aquatic organisms. As a ubiquitous pollutant in aquatic environments, polystyrene nanomicroplastics (PSNPs) have shown strong adsorption capacity due to their large surface area. Therefore, the potential joint effects of ammonia and PSNPs need to be clarified.

The Critical Role of Lipid Metabolism in Health and Diseases.

Lipids make up 10-20% of the human body and serve as crucial energy storage substances as well as essential components of cell membranes [...

Effects of Illumination Color on Hypothalamic Appetite-Regulating Gene Expression and Glycolipid Metabolism.

Irregular illumination is a newly discovered ambient factor that affects dietary and metabolic processes. However, the effect of the modulation of long-term light exposure on appetite and metabolism remains elusive. Therefore, in this current study, we systematically investigated the effects of up to 8 weeks of exposure to red (RL), green (GL), and white light (WL) environments on appetite, food preferences, and glucose homeostasis in mice on both high-fat and low-fat dietary patterns.

Novel Nuclease MbovP701 with a Yqaj Domain Is Interrelated with the Growth of .

() is characterized by a reduced genomic size and limited synthetic capacity, including the inability to synthesize nucleotides de novo, relies on nucleases for nutrient acquisition and survival. A number of nucleases have been implicated in pathogenicity, facilitating substrate degradation and contributing to DNA repair mechanisms that enhance bacterial persistence. The present study confirmed that the T5.

Phage vB_SpuM_X5: A Novel Approach to Reducing Biofilms with Implications for Food Safety.

, a prevalent foodborne pathogen, poses a significant social and economic strain on both food safety and public health. The application of phages in the control of foodborne pathogens represents an emerging research area. In this study, phage vB_SpuM_X5 (phage X5) was isolated from chicken farm sewage samples.

Regulation of Isoleucine on Colonic Barrier Function in Rotavirus-Infected Weanling Piglets and Analysis of Gut Microbiota and Metabolomics.

Rotavirus (RV) is a significant contributor to diarrhea in both young children and animals, especially in piglets, resulting in considerable economic impacts on the global pig industry. Isoleucine (Ile), a branched-chain amino acid, is crucial for regulating nutrient metabolism and has been found to help mitigate diarrhea. This study aimed to assess the impact of isoleucine supplementation in feed on colonic barrier function, colonic microbiota, and metabolism in RV-infected weanling piglets.

The Catalytic Mechanism of Key Enzymes Involved in the Synthesis of Ergothioneine in .

C-S lyase is a crucial enzyme responsible for the formation of sulfur-containing flavor compounds in . We investigated the involvement of C-S lyase in the synthesis of ergothioneine (EGT) in , a high-producing edible mushroom. Through experimental and computational approaches, we identified 2, a C-S lyase, as a key enzyme involved in EGT synthesis in .

Application of an Anchor Mapping of Alien Chromosome (AMAC) Fragment Localization Method in the Identification of Radish Chromosome Segments in the Progeny of Rape-Radish Interspecific Hybrids.

Rape () is an important oilseed crop widely cultivated worldwide. Due to its relatively short evolutionary and domestication history, its intra-species genetic diversity is limited. Radish (), belonging to a different genus but the same family as , possesses an abundance of excellent gene resources.