5-Methyl-2-[3-(4-phenylthiazol-2-yl)triazenyl]benzenesulfonic acid as a chromogenic reagent of N-cetylpyridinium chloride Synthesis, mechanism and analytical application.

Despite that triazene reagents have been widely used for spectrophotometric determinations of cationic surfactants, the mechanism underlying such applications has yet to be studied. We report the synthesis of a new triazene reagent 5-methyl-2-[3-(4-phenylthiazol-2-yl)triazenyl]benzenesulfonic acid (MPTTBSA) and its interaction with N-cetylpyridinium chloride (CPC). The reagent was synthesized by coupling 4-methyl-2-sulfobenzenediazonium salt with 2-amino-4-phenylthiazole.

Synthesis of a novel bistriazene reagent 4,4'-bis[3-(4-phenylthiazol-2-yl)triazenyl]biphenyl and its highly sensitive color reaction with mercury(II).

We report the synthesis of a novel bistriazene, 4,4'-bis(3-(4-phenylthiazol-2-yl)triazenyl)biphenyl (BPTTBP), and its highly sensitive color reaction with Hg(2+). The new reagent was synthesized in good yield by coupling 2-amino-4-phenylthiazole with 4,4'-biphenyldiamine bisdiazonium salt. Using a blend of surfactants N-cetylpyridinium chloride (CPC) and polyethylene glycol n-octanoic phenyl ether (OP) as a micelle sensitizer, the red colored reagent assembles with Hg(2+) in pH 9.

[Morphogenesis in formative process in vitro from Rehmannia glutinosa].

Objective: To study the morphogenesis in formative process of tuberous root in vitro from Rehmannia glutinosa and compare the anatomical shape of tuberous root with nature term R. glutinosa.

Method: Tuberous roots of different vegetal phase were cut and dyed, then made into paraffin cuts and observed microscope.

[Studies on petal tissue culture of the traditional Chinese medicine Chrysanthemum morifolium].

Objective: To set up the optimums for the petal tissue culture of Chrysanthemum morifolium cultivated in Anhui Province.

Method: Petals were cut and cultured onto the same kind of media on different sides and different kinds of media on the same side, and induced to form the whole plantlet.

Result And Conclusion: All the media used could induce callus, while the results of callus regeneration was distinct.

[Progress on research of tissue culture of Rehmannia glutinosa].

Objective: To review the development of tissue culture on Rehmannia glutinosa.

Method: Documentaries at home and abroad were consulted.

Result And Conclusion: The tissue culture conditions of R.

[The change of activity of protective enzyme around sprout tumble of Pinellia ternate under high temperature stress].

Objective: To study the change of the activity of protective enzyme to avoid sprout tumble of Pinellia ternata.

Method: A high temperature stress was given to P. ternata.

[Studies on callus induced from leaves and plantlets regeneration of the traditional Chinese medicine Chrysanthemum morifolium].

Objective: To set up the optimums for the leaf tissue culture of Chrysanthemum morifolium cultivated in Anhui Province.

Method: Leaves were cut and cultured onto the same kind of media on different sides and defferent kinds of media on the same side, and induced to form the whole plantlet.

Result And Conclusion: All the media used could induce callus, while the results of callus redifferentiation were very distinct.

[Study on plant tissue culture of Cornus officinalis].

Objective: To set up the optimums of tissue culture for Cornus officinalis.

Method: A section of stems of the fine varieties of Cornus officinalis were strictly sterilized, cultivated and geminated in medium with addition of different kinds of hormone.

Result And Conclusion: The WPM medium with 6-BA 2.

[Studies on organ phytomorphology of leaves from Rehmannia glutinosa].

Objective: To set up the fittest medium and optimum condition for leaves from Rehmannia glutinosa to form regenerated plantlets directly and to induce microtubers.

Method: Leaves of different phases obtained from the virus-free plantlets were incubated in some kinds of media on the back side.

Result And Conclusion: The fittest medium for the formation of regenerated plantlet was MS + 6-BA 2.

[Microtuber induction in vitro from Rehmannia glutinosa].

Objective: To set up the fittest medium and optimum condition for plantlets to form microtubers.

Method: Plant hormones, concentration of sucrose, active carbon and light time influenced formation of adventitious root and microtuber in vitro from plantlet of Rehmannia glutinosa (85-5).

Result And Conclusion: The plantlets were cultivated on the medium of microtuber induction after roots were induced on the 1/2MS medium with IBA 1 mg.

[Study on stem-tip tissue culture of the traditional Chinese medicine Chrysanthemum morifolium].

Objective: To set up the optimums for the stem-tip tissue culture of Chrysanthemum morifolium cultivated in Anhui Province.

Method: Small sections (about 0.5 mm in length) from the stem-tips were isolated and inoculated with different media, and induced to form the whole plantlet formation.