[Target-specific cytotoxic activity of recombinant fusion toxin C-CPE-ETA' against CLDN-3,4-overexpressing ovarian cancer cells].

Objective: The aim of this study was to explore the possibility of creating a toxin, C-CPE-ETA', by fusing C-terminal high affinity binding domain of CPE (C-CPE) with a truncated form of Pseudomonas aeruginosa exotoxin A (ETA') and to examine whether C-CPE-ETA' could specifically target CLDN-3, 4 molecule and the targeted toxin was cytotoxic against CLDN-3,4-overexpressing ovarian cancer.

Methods: CLDN-3 and CLDN-4 expressions were analyzed at the mRNA level in three ovarian cancer cell lines and epithelial ovarian cancer tissues from 20 patients. After transforming an expression plasmid of C-CPE-ETA' into E.

Expression and significance of factors related to angiogenesis in choroidal melanoma.

Aim: TO INVESTIGATE EXPRESSION OF FACTORS RELATED TO ANGIOGENESIS: HIF-1α, iNOS, COX-2 and VEGF in choroidal melanoma and its clinical significance.

Methods: Fifty samples of choroidal melanoma and 15 samples of melanocytic nevi of the eyelid identified by pathology were collected. Immunohistochemistry SP method was used to examine the expression of HIF-1α, iNOS, COX-2 and VEGF in these samples.

[Expression of mesothelin mRNA and protein in ovarian carcinomas].

Objective: To investigate the expression of mesothelin (MESO) mRNA and protein and its significance in ovarian carcinomas.

Methods: Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry were used to detect the expression level of MESO mRNA and protein, respectively, in 124 samples of ovarian tumor and normal tissues, including 84 epithelial ovarian carcinomas, 12 borderline ovarian tumors, 16 benign ovarian tumors and 12 normal ovarian tissues.

Results: The expression of MESO mRNA and protein in epithelial ovarian carcinomas (1.

[Expression of inducible nitric oxide synthase and macrophage in human placenta of pregnancy induced hypertension syndrome].

Objective: To investigate the expression of inducible nitric oxide synthase (iNOS) and macrophage (CD(68)) in the placenta of the patients with pregnancy induced hypertension syndrome(PIH) and the role of them in the pathogenesis of PIH.

Methods: Immunohistochemical technique (SABC) was used for immunostaining and detecting inducible nitric oxide synthase and CD(68) in the placentas of thirty patients of PIH (two patients of mild PIH; seven moderate PIH; twenty-one severe PIH) and twenty-two normal pregnancies (the control group).

Results: The level of inducible nitric oxide synthase and CD(68) in the placenta of patients of PIH were (2.