Geographical Isolation, Buried Depth, and Physicochemical Traits Drive the Variation of Species Diversity and Prokaryotic Community in Three Typical Hypersaline Environments.

The prokaryotic community composition, species diversity and the distribution patterns at various taxonomic levels in a salt lake (Chaka salt lake), solar salterns (Taipei saltworks and Dongfang saltworks), and salt mines (Yuanyongjing salt mine, Xiangyan salt mine, and Dinyuan salt mine) were investigated using clone library or Illumina MiSeq sequencing. The clone library approach revealed that the salt lake harbors low species diversity (H' = 0.98) as compared to the solar saltern (H' = 4.

Synthesis and biological evaluation of novel 3-benzylcoumarin-imidazolium salts.

A series of novel 3-benzylcoumarin-imidazolium salts were prepared and evaluated in vitro against a panel of human tumor cell lines. The results showed that the existence of 5,6-dimethyl-benzimidazole ring and substitution of the imidazolyl-3-position with a naphthylacyl group were vital for modulating cytotoxic activity. Notably, compound 38 was found to be the most potent derivative with IC values of 2.

Genome-wide identification and expression profile of the MADS-box gene family in Erigeron breviscapus.

The MADS-box gene family encodes transcription factors with many biological functions that extensively regulate plant growth, development and reproduction. Erigeron breviscapus is a medicinal herb used widely in traditional Chinese medicine, and is believed to improve blood circulation and ameliorate platelet coagulation. In order to gain a detailed understanding of how transcription factor expression may regulate the growth of this potentially important medicinal plant, a genome-wide analysis of the MADS-box gene family of E.

Aptamer-Functionalized DNA Origami for Targeted Codelivery of Antisense Oligonucleotides and Doxorubicin to Enhance Therapy in Drug-Resistant Cancer Cells.

Drug resistance is a major obstacle to the efficient therapy of drug-resistant cancer. To overcome this problem, we constructed a multifunctional DNA origami-based nanocarrier for codelivery of a chemotherapeutic drug (doxorubicin, Dox) and two different antisense oligonucleotides (ASOs; B-cell lymphoma 2 (Bcl2) and P-glycoprotein (P-gp)) into drug-resistant cancer cells for enhanced therapy. To increase the targeting ability of origami, staple strands with 5'-end extended MUC1 sequences were used in the preparation of aptamer-functionalized origami carrying ASOs (Apt-origami-ASO).

A simple and convenient synthesis of 3-salicyloylquinoline-4-carboxylic esters from chromone and isatin.

A simple and convenient synthesis of 3-salicyloylquinoline-4-carboxylic esters has been developed through an AlCl-catalyzed reaction of easily available Baylis-Hillman adducts from chromones and isatin-derivatives. This reaction involves esterification, cyclization and ring opening in a one-step process, and provides an efficient approach for easy access to a series of valuable salicyloylquinoline derivatives with high yields. Moreover, this protocol offers several advantages, such as availability of starting materials, economic availability, operational simplicity and mild reaction conditions.

Halobellus captivus sp. nov., an extremely halophilic archaeon isolated from a subterranean salt mine.

An extremely halophilic archaeon, strain ZY21, was isolated from a subterranean rock salt sample in Yunnan, China. Colonies of strain ZY21 on nutrient-rich agar plates are orange, wet and transparent. Cells are pleomorphic, motile, Gram-stain negative and lyse in distilled water.

Three-Component One-Pot Synthesis of Highly Functionalized Bis-Indole Derivatives.

In this study, we detail the development of a concise and efficient three-component protocol for the regioselective synthesis of highly functionalized bis-indoles through a one-pot, two-step sequential process starting from enaminones , indoles , and acenaphthylene-1,2-dione that is catalyzed by piperidine and -methyl benzenesulfonic acid. This protocol has several advantages including simplicity of experimental operation, high efficiency of bond formation, ready availability and low cost of starting materials, environmentally benign conditions, and target molecular diversity.

Halorubrum amylolyticum sp. nov., a novel halophilic archaeon isolated from a salt mine.

A pleomorphic and non-motile halophilic archaeon forming light-red pigmented colonies, strain ZC67, was isolated from the Yuanyongjing Salt Mine, Yunnan, China. Based on similarity search and phylogenetic analysis of the 16S rRNA gene sequence, strain ZC67 belongs to the genus Halorubrum and is closely related to the species of Halorubrum (Hrr.) saccharovorum JCM 8865, Hrr.

Blue-White Colony Selection of Virus-Infected Isogenic Recipients Based on a Chrysovirus Isolated from Penicillium italicum.

Mycoviruses have been found to infect more than 12 species of Penicillium, but have not been isolated from Penicillium italicum (P. italicum). In this study, we isolated and characterized a new double-stranded RNA (dsRNA) virus, designated Penicillium italicum chrysovirus 1 (PiCV1), from the citrus pathogen P.

gen. nov., sp. nov., an extremely halophilic archaeon isolated from a subterranean salt deposit.

A halophilic archaeon, strain H22, was isolated from a subterranean salt deposit sampled at Yunnan salt mine, PR China. Colonies of strain H22 were light pink-pigmented. Cells were coccus, non-motile, Gram-stain-negative, and did not lyse in distilled water.

Determination of Pyrethroids in Sample by High-Performance Liquid Chromatography Using Ultrasound-Assisted Magnetic Solid-Phase Extraction.

A novel ultrasound-assisted magnetic solid-phase extraction (UA-MSPE) was developed for the separation/preconcentration of trace amounts of pyrethroids (fenpropathrin, fenvalerate, deltamethrin, and bifenthrin) in sample using carbon nanotubes based on FeO magnetic nanoparticles (FeO@CNT MNPs), and high-performance liquid chromatography-UV is described. High recoveries of pyrethroids were obtained at a low MNPs concentration because sonication enhances the contact chances between magnetic nanoparticles and extractable analytes and promotes the extractability of the MSPE process. After the extraction, the adsorbent can be conveniently separated from the sample solution by an external magnet, and the adsorbed analytes were eluted from magnetic FeO@CNT.

Molecular Characterization of a Chrysovirus Isolated From the Citrus Pathogen and Related Fungicide Resistance Analysis.

sp. are damaging to a range of foods and fruits including citrus. To date, double-stranded (ds)RNA viruses have been reported in most species but not in citrus pathogen .

Haloterrigena salifodinae sp. nov., an extremely halophilic archaeon isolated from a subterranean rock salt.

A novel extremely halophilic strain, designated ZY19, was isolated from a rock salt sample from Yunnan salt mine, PR China. Strain ZY19 is neutrophilic, non-motile and requires at least 10% (w/v) NaCl for growth. Optimal growth is observed at 20-25% (w/v) NaCl, pH 7.

Molecular cloning and expression analysis of SRLK1 gene in self-incompatible Asteraceae species Erigeron breviscapus.

Based on the transcriptome data, using RACE techniques, we cloned the full-length EbSRLK1 gene in a medicinal, self-incompatible Asteraceae species, Erigeron breviscapus. Bioinformatics approaches were used to analyze the DNA and protein sequences, physical and chemical properties, and domains of the encoded protein. The full-length EbSRLK1 cDNA is 2891 base pairs (bp) with an open reading frame (ORF) of 2634 bp, which encodes the EbSRLK1 protein with 878 amino acids and an estimated molecular weight of 98.

Diversity, community distribution and growth promotion activities of endophytes associated with halophyte Murr.

The purpose of this study was to investigate the composition, diversity, distribution, and growth promotion activity of endophytic bacteria isolated from Murr. Consequently, a total of 109 endophytic bacteria affiliated to 3 phyla, 12 orders and 36 genera were isolated using nine different selective media, from which, was the dominant taxon containing seven orders at the phylum level; showed the highest diversity containing 12 genera at the family level. Based on PAST and SPSS analysis, species diversity and abundance were mostly isolated from nutritious soil condition (22 genera) and root tissue (27 genera).

Halalkalicoccus subterraneus sp. nov., an extremely halophilic archaeon isolated from a subterranean halite deposit.

An extremely halophilic archaeon, designated strain GSM28, was isolated from a subterranean halite deposit in a Yunnan salt mine, China. Cells of the strain were observed to be cocci, non-motile and Gram-variable, and to require at least 15% (w/v) NaCl for growth (optimum 20%). Growth was found to occur in the ranges of 20-45 °C (optimum 42 °C) and pH 6.

Botryobacter ruber gen. nov., sp. nov., a novel member of the family Hymenobacteraceae.

Strain SYSU D60016, a rod-shaped bacterium that tends to form clusters, was isolated from a soil sample collected from the Gurbantunggut desert, China. Cells were Gram-stain-negative, catalase-negative and oxidase-positive. The 16S rRNA gene sequence of strain SYSU D60016 shared less than 94 % sequence identity with members of the family Hymenobacteraceae.

Modelling and analysis of HFMD with the effects of vaccination, contaminated environments and quarantine in mainland China.

Currently, hand, foot, and mouth disease (HFMD) is widespread in mainland China and seriously endangers the health of infants and young children. Recently in mainland China, preventing the spread of the disease has entailed vaccination, isolation measures, and virus clean-up in the contaminated environment. However, quantifying and evaluating the efficacy of these strategies on HFMD remains challenging, especially because relatively little research analyses the impact of EV71 vaccination for this disease.

Flaviflagellibacter deserti gen. nov., sp. nov., a novel member of the order Rhizobiales isolated from a desert soil.

A motile, rod-shaped and yellow coloured proteobacterium, designated strain SYSU D60017, was isolated from a desert soil sample. The bacterium was found to be an obligately aerobic, mesophilic and neutrophilic chemo-heterotroph. Cells were observed to be Gram-stain negative, catalase positive and oxidase positive.

Unigene-based RNA-seq provides insights on drought stress responses in Marsdenia tenacissima.

Marsdenia tenacissima is a well-known anti-cancer medicinal plant used in traditional Chinese medicine, which often grows on the karst landform and the water conservation capacity of land is very poorly and drought occurrences frequently. We found M. tenacissima has strong drought resistance because of continuousdrought16 d, the leaves of M.

Halorubrum glutamatedens sp. nov., a Halophilic Archaeon Isolated from a Rock Salt.

An extremely halophilic archaeon, strain ZY8, was isolated from a rock salt of Yunnan salt mine. It was able to grow at 12-30% (w/v) NaCl (optimum, 15-20%), pH 7.0-9.

Halococcus salsus sp. nov., a novel halophilic archaeon isolated from rock salt.

Two pink-pigmented halophilic archaea, designated strains ZJ1 and J81, were isolated from rock salt of Yunnan Salt Mine, China, and commercial salt imported from Bolivia, respectively. Cells were non-motile, coccoid, approximately 0.8-1.

Desertimonas flava gen. nov., sp. nov. isolated from a desert soil, and proposal of Ilumatobacteraceae fam. nov.

A non-motile, coccobacilli-shaped and yellow-coloured bacterium, designated strain SYSU D60003, was isolated from a desert soil sample. Cells were Gram-stain-positive, catalase-negative and oxidase-positive. The whole cell hydrolysates contained ll-diaminopimelic acid as the diagnostic amino acid.

Construction of Fully Substituted 2-Pyridone Derivatives via Four-Component Branched Domino Reaction Utilizing Microwave Irradiation.

Microwave irradiation, four-component branched domino reaction of methyl acetoacetate/2,4-pentanedione, diethyl malonate, triethyl orthoformate and amines offering an extremely efficient strategy for the construction of fully substituted 2-pyridone derivatives under sustainable conditions is established. This self-sorting branched domino transformation is proposed to proceed separate through N-nucleophilic addition and imine-enamine tautomerization/condensation reaction generated from enamino ester and diethyl ethoxymethylenemalonate, and then would be subjected to an aza-ene reaction and intramolecular cyclization mechanism to afford the 2-pyridones with only water and ethanol as byproducts. The simple experimental procedure, high bond-forming efficiency, step and atom economy, inexpensive readily available starting materials, moderate to excellent yields, and good functional group compatibility are other noteworthy advantages of this method.