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Protocol of mesenchymal stem cell inoculation to nonwoven fabric scaffold.
Cytotechnology
June 2019
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido TechUniversity, Kita-ku, N13W8, Sapporo, 060-8628, Japan.
To obtain a large number of human mesenchymal stem cell (hMSCs) for allograft, nonwoven fabrics (NWF) were used as a cell culture scaffold. NWF are three-dimensional fiber aggregates formed by heat bonding and have a high surface area for cell adhesion and elongation. Inoculation hMSC was done to a center of NWF disc (diameter, 15.
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