1 results match your criteria: "Histoinformatics and MIND Centres[Affiliation]"
Enhanced yellow fluorescent protein photoconversion to a cyan fluorescent protein-like species is sensitive to thermal and diffusion conditions.
J Biomed Opt
September 2009
Aarhus University, Stereology and Electron Microscopy Research Laboratory, Histoinformatics and MIND Centres, Ole Worms Alle 1185, DK-8000 Aarhus C., Denmark.
Article Synopsis
- Research on fluorescent proteins is yielding new mutations that can change color when exposed to UV or blue light, with a discovery of EYFP converting to a CFP-like protein under green light.
- The photoconversion process is influenced by temperature and occurs mainly in fixed, open cell samples, potentially creating a bright, stable cyan-like fluorescent protein.
- Variation in photoconversion efficiency suggests it complicates pbFRET analysis, so samples should be discarded if photoconversion is detected to ensure accurate results.